Molecular modulation and gene therapy in choroidal neovascularization.

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1. Molecular modulation in choroidal neovascularizationChoroidal neovascularization (CNV) was induced in monkey and rat eyes by intense laser photocoagulations of the retina in the posterior pole. In experimental CNV, expression of mRNA of b-fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor (TGF)-β, and their receptors were revealed, using in situ hybridization and immunohistochemistry, markedly in the early stage in developing of CNV, and slightly in the late stage. These results suggest development and regression of CNV were modulated by these cytokines.2. Effect of Interferron β on development of CNVLocal (subtenon) or systemic administration of Interferron (IFN)-β inhibited development of CNV.Involution of CNV were due to enclosure by proliferatin of the retinal pigment epithelium (RPE). IFN-β upregulated expression of angiogenicfactors such as bFGF and VEGF in CNV.3. Possibility of gene therapy in CNVA vector of combination of hemagglutinating Virus of Japan (HVJ) and liposome was enveloped with antisense-olignucleotide or antisense plasmid, then intoroduced to CNV or RPE in vivo. They were successfully transferred in the target cells.These results show gene therapy with HVJ-liposome method will be achieved effectively in CNV treatment.