Expression of basic FGF,FGF receptor, TGF-beta, and VEGF in experimental choroidal neovascularization

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Development and regression of choroidal neovascularizaion (ChNV) may be regulated by growth factors and cytokines. To reveal existence and localization of basic fibroblast growth factor (b FGF), FGF receptor, transforming growth factor (TGF), vascular endothelial growth factor (VEGF) in ChNV,we demonstrated expression of mRNAs of them with in situ hybridization method and immunohistochemical study.ChNV were produced in pigmented rat with intense photocoagulation (PC). Antisense and sense riboprobes were prepared from rat b FGF,FGF receptor, TGF-beta_1, mouse TGF-beta_2, TGF-beta_3, VEGF cDNAs. In situ hybridization was performed on sections of the retina and choroid following PC.Basic FGF,FGF receptor, TGF-beta_1, TGF-beta_2, and VEGF,mRNAs were detected in the ganglion cell layr in normal rat retina, though TGF-beta_3 was not detected. In developing stage of ChNV after PC,expression of b FGF,FGF receptor and VEGF mRNAs were marked in the retinal pigment epithelial cells, and vascular endothelial cells. Expression of TGF-beta_1 and TGF-beta_2, mRNAs were also detected. However, these factors were no longer detected in regressing stage of ChNV.These results suggest endogenous b FGF,and VEGF promotes development of choroidal neovascularization, and TGF-beta_1 and beta_2 appears to be associated in regulation of ChNV,in vivo.