Expression of basic FGF,FGF receptor, TGF-beta, and VEGF in experimental choroidal neovascularization

脉络膜新生血管（ChNV）的形成和消退可能受生长因子和细胞因子的调控。为了揭示碱性成纤维细胞生长因子（b FGF）、FGF受体、转化生长因子（TGF）、血管内皮生长因子（VEGF）在ChNV中的存在和定位，我们采用原位杂交法和免疫组化研究证实了它们的mrna的表达。采用强光凝法制备大鼠ChNV。从大鼠FGF、FGF受体、tgf - β _1、小鼠tgf - β _2、tgf - β _3、VEGF cdna制备反义和正义核糖探针。对PC后视网膜和脉络膜切片进行原位杂交。正常大鼠视网膜神经节细胞层检测到基本FGF、FGF受体、tgf - β _1、tgf - β _2和VEGF mrna，未检测到tgf - β 3。在PC后ChNV的发育阶段，视网膜色素上皮细胞和血管内皮细胞中有b - FGF、FGF受体和VEGF mrna的表达。同时检测TGF-beta_1和TGF-beta_2 mrna的表达。然而，这些因素在ChNV的回归阶段不再检测到。这些结果提示内源性FGF和VEGF促进脉络膜新生血管的形成，tgf - β _1和β _2可能与体内ChNV的调节有关。

Development and regression of choroidal neovascularizaion (ChNV) may be regulated by growth factors and cytokines. To reveal existence and localization of basic fibroblast growth factor (b FGF), FGF receptor, transforming growth factor (TGF), vascular endothelial growth factor (VEGF) in ChNV,we demonstrated expression of mRNAs of them with in situ hybridization method and immunohistochemical study.ChNV were produced in pigmented rat with intense photocoagulation (PC). Antisense and sense riboprobes were prepared from rat b FGF,FGF receptor, TGF-beta_1, mouse TGF-beta_2, TGF-beta_3, VEGF cDNAs. In situ hybridization was performed on sections of the retina and choroid following PC.Basic FGF,FGF receptor, TGF-beta_1, TGF-beta_2, and VEGF,mRNAs were detected in the ganglion cell layr in normal rat retina, though TGF-beta_3 was not detected. In developing stage of ChNV after PC,expression of b FGF,FGF receptor and VEGF mRNAs were marked in the retinal pigment epithelial cells, and vascular endothelial cells. Expression of TGF-beta_1 and TGF-beta_2, mRNAs were also detected. However, these factors were no longer detected in regressing stage of ChNV.These results suggest endogenous b FGF,and VEGF promotes development of choroidal neovascularization, and TGF-beta_1 and beta_2 appears to be associated in regulation of ChNV,in vivo.