BASIC STUDY ON BIOPHOSPHORITE PRODUCTION BY ENHANCING BACTERIAL ABILITY TO SYNTHESIZE PHOSPHATE BIOPOLYMERS.

通过增强细菌合成磷酸盐生物聚合物的能力来生产生物亚磷酸盐的基础研究。

• 批准号: 10450312
• 负责人: OHTAKE Hisao
• 金额: $ 6.85万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10450312/
• 关键词: polyphosphate; phosphorus recycling; biological phosphate removal; biophosphorite; ポリリン酸顆粒; 生物脱リン; 富栄養化; 資源問題; 好気嫌気法; バイオリン; 活性汚泥; 細菌; 遺伝子; 環境バイオテクノロジー

Phosphorus in wastewaters causes environmental pollution. On the other hand, phosphorus is essential in fertilizers for food production. However, natural phosphorus resources are being dissipated. The purpose of this research is to develop a bioprocess for recovering phosphorus from activated sludge, intending to prevent environmental pollution and to avert a crisis in phosphorus availability. In enhance biological phosphate removal, sludge bacteria accumulate large quantities of polyphosphate (polyP). The polyP could be released from activated sludge simply by heating at 70oC for one or two h. Released polyP could be easily precipitated by the addition of calcium chloride without pH control. The phosphorus content of the precipitates surpassed those of natural phosphorite deposits (NPD). Conversely, they had a relatively low calcium content, when compared with those of NPD.The bench-scale wastewater system, coupled with a process for phosphorus recovery, showed the high, stable performance for at least two months of operation. The present system will make a great contribution to both phosphorus recycling and eutrophication control.

废水中的磷会造成环境污染。另一方面，磷在粮食生产的肥料中是必不可少的。然而，天然磷资源正在消失。本研究的目的是开发一种从活性污泥中回收磷的生物工艺，以防止环境污染和避免磷的可用性危机。在强化生物除磷过程中，污泥细菌积累了大量的聚磷酸盐（polyP）.活性污泥在70 ℃加热1 ~ 2 h即可释放聚磷。释放的聚P可以容易地通过添加氯化钙沉淀而无需pH控制。沉淀物的磷含量超过了天然磷矿床（NPD）的磷含量。相反，他们有一个相对较低的钙含量，当与NPD.The实验室规模的废水系统，再加上磷回收过程中，表现出高，至少两个月的运行稳定的性能。该系统将为磷的循环利用和富营养化控制做出巨大贡献。

项目成果

大竹久夫: "リン除去細菌の開発と活性汚泥からのポリリン酸熱回収技術"醸造協会誌. 95. 23-28 (2000)

Hisao Otake：“活性污泥除磷细菌和多磷酸热回收技术的开发”酿造协会杂志 95. 23-28 (2000)。

大竹久夫: "バイオレメディエーションを技術を活用する汚染環境の修復-リン資源の循環再利用のためのバイオテクノロジー"環境科学会誌. 12. 433-441 (1999)

Hisao Otake：“利用生物修复技术修复受污染的环境 - 磷资源循环再利用的生物技术”环境科学学会杂志 12. 433-441 (1999)。

Hisao Ohtake: "Environmental Monitoring and Biodiagnostics of Hazardous"Contaminants,Kluwer Academic Publishers(in press)..

Hisao Ohtake：“危险污染物的环境监测和生物诊断”，Kluwer 学术出版社（出版中）..

Kazuya Imazu: "Enhanced utilization of phosphonate and phosphite by kldosiella aerogenes" Applied and Environmental Microbiology. 64. 3754-3758 (1998)

Kazuya Imazu：“产气克氏杆菌增强对膦酸盐和亚磷酸盐的利用”应用和环境微生物学。

Akio Kuroda: "Inorganic polyphosphate kinase is requied to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli."Proc.Natl.Acad.Sci.USA.. 96. 14264-14269 (1999)

Akio Kuroda：“需要无机多磷酸激酶来刺激蛋白质降解并适应大肠杆菌的氨基酸饥饿。”Proc.Natl.Acad.Sci.USA.. 96. 14264-14269 (1999)