Signal transduction map and direct control of cellular actvivities of bacteria

信号转导图谱和细菌细胞活性的直接控制

• 批准号: 06650919
• 负责人: OHTAKE Hisao
• 金额: $ 1.28万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06650919/
• 关键词: Signal Transduction; Bacteria; Gene; Chemotaxis; Regulation; リン酸; 走化性

P.aeruginosa is attracted toward P_<i'> when the cells are starved for P_i. Regulation of P_i taxis is not dependent on PhoB and PhoR but requires PhoU.The P.aeruginosa pst gene products, which are required for P_i-specific transport, are also involved in the negative regulation of P_i taxis. However, it is unknown whether a positive regulator exists for P_i taxis. Nothing is known about the P_i chemoreceptor at this time. Five chemotaxis genes, including the cheY,cheZ,cheA,cheB and cheJ,have been identified in a large gene cluster of P.aeruginosa. The predicted proteins of the first four genes resemble known E.coli Che proteins. However, the CheJ protein has no significant homology with any known protein sequences. This putative protein may play an unexpected role in the chemotactic signal-transduction pathway in P.aeruginosa. Three possible chemotactic transducer genes, pctA,pctB and pctC,have also been cloned and sequenced. The gene products have structural homology with the enteric MCPs. Our future work on the chemotaxis of P.aeruginosa will be directed at (i) identifying P_i chemoreceptor ; (ii) seeking out additional chemotaxis genes ; and (iii) cloning chemotactic transducer genes that remain to be identified.

当细胞饥饿的P_I饥饿时，P.Aeruginosa被吸引到P_ <i'>。 P_I出租车的调节不依赖于PHOB和PHOR，而是需要Phou。P.AeruginosaPST基因产品（P_I特异性运输所需）也参与了P_I出租车的负调节。但是，尚不清楚P_I出租车是否存在积极的调节器。目前，P_I化学感受器尚无任何了解。五个趋化性基因，包括Chey，Chez，Chea，Cheb和Chej，在大型P.Aeruginosa基因群中被鉴定出来。前四个基因的预测蛋白质类似于已知的大肠杆菌蛋白质。然而，CHEJ蛋白与任何已知蛋白序列没有显着同源性。这种推定的蛋白质可能在p.aeruginosa的趋化信号转导途径中起意外的作用。也已克隆并测序了三个可能的趋化传感器基因PCTA，PCTB和PCTC。该基因产物与肠MCP具有结构同源性。我们未来在p.aeruginosa趋化性方面的工作将针对（i）识别P_I化学感受器； （ii）寻找其他趋化基因； （iii）克隆趋化传感器基因，尚待鉴定。

项目成果

Nikata.,T.,Sakai,Y.,Shibata,K.,Kato,J.,Kuroda,A.and Ohtake,H.: "Molecular analysis of the phosphate-specific transport (pst) operon of Pseudomonas aeruginosa." Mol.Gen.Gent.(印刷中).

Nikata., T.、Sakai, Y.、Shibata, K.、Kato, J.、Kuroda, A. 和 Ohtake, H.：“铜绿假单胞菌 Mol 磷酸盐特异性转运 (pst) 操纵子的分子分析。” .Gen.Gent.（正在出版）。

Kato, J., Sakai, Y.Nikata, T., and Ohtaka, H.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis." J.Bacteriol.176. 5874-5877 (1994)

Kato, J.、Sakai, Y.Nikata, T. 和 Ohtaka, H.：“参与磷酸盐趋向性负调节的铜绿假单胞菌基因的克隆和表征。”

A.MASDUKi: "Isolation and characterization of chemotaxis mutants and genes of pseudomonas aeruginosa" J.Bacteriology. (印刷中).

A.MASDUKi：“铜绿假单胞菌趋化突变体和基因的分离和表征”J.Bacteriology（正在出版）。

Kuroda, A., Kumano, T., Taguchi, K., Nikata, T., Kato, J.and Ohtake, H.: "Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa." J.Bacteriol.177. 7019-7125 (1995)

Kuroda, A.、Kumano, T.、Taguchi, K.、Nikata, T.、Kato, J. 和 Ohtake, H.：“铜绿假单胞菌趋化转导基因的分子克隆和表征。”

Ikegami,S.,Imai.I.,Kato,J.and Ohtake,H.: "Chemotaxis toward inorganic phosphate in the red tide alga Chattonella antigua." J.Plankton Res.,. 17. 1586-1590 (1995)

Ikegami,S.、Imai.I.、Kato,J. 和 Ohtake,H.：“赤潮藻安提瓜查托氏菌对无机磷酸盐的趋化性”。