Signal transduction map and direct control of cellular actvivities of bacteria

信号转导图谱和细菌细胞活性的直接控制

• 批准号: 06650919
• 负责人: OHTAKE Hisao
• 金额: $ 1.28万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06650919/
• 关键词: Signal Transduction; Bacteria; Gene; Chemotaxis; Regulation; リン酸; 走化性

P.aeruginosa is attracted toward P_<i'> when the cells are starved for P_i. Regulation of P_i taxis is not dependent on PhoB and PhoR but requires PhoU.The P.aeruginosa pst gene products, which are required for P_i-specific transport, are also involved in the negative regulation of P_i taxis. However, it is unknown whether a positive regulator exists for P_i taxis. Nothing is known about the P_i chemoreceptor at this time. Five chemotaxis genes, including the cheY,cheZ,cheA,cheB and cheJ,have been identified in a large gene cluster of P.aeruginosa. The predicted proteins of the first four genes resemble known E.coli Che proteins. However, the CheJ protein has no significant homology with any known protein sequences. This putative protein may play an unexpected role in the chemotactic signal-transduction pathway in P.aeruginosa. Three possible chemotactic transducer genes, pctA,pctB and pctC,have also been cloned and sequenced. The gene products have structural homology with the enteric MCPs. Our future work on the chemotaxis of P.aeruginosa will be directed at (i) identifying P_i chemoreceptor ; (ii) seeking out additional chemotaxis genes ; and (iii) cloning chemotactic transducer genes that remain to be identified.

当细胞缺乏P_i时，铜绿假单胞菌被P_i '吸引。铜绿假单胞菌Pst基因产物也参与了P_i的负调控，它们是P_i转运所必需的。然而，P_i出租车是否存在正调节器是未知的。目前对P_i化学感受器还不清楚。在铜绿假单胞菌的一个大的基因簇中，已经鉴定出5个趋化基因，包括cheY、cheZ、cheA、切布和cheJ。前四个基因的预测蛋白类似于已知的大肠杆菌Che蛋白。然而，CheJ蛋白与任何已知的蛋白质序列没有显著的同源性。这种推定的蛋白质可能发挥了意想不到的作用，在P.aerodosa.的趋化信号转导途径。三个可能的趋化性转导基因，pctA，pctB和pctC，也被克隆和测序。基因产物与肠MCP具有结构同源性。我们今后的工作将主要集中在鉴定P_i化学受体、寻找新的趋化基因和克隆尚待鉴定的趋化转导基因。

项目成果

Nikata.,T.,Sakai,Y.,Shibata,K.,Kato,J.,Kuroda,A.and Ohtake,H.: "Molecular analysis of the phosphate-specific transport (pst) operon of Pseudomonas aeruginosa." Mol.Gen.Gent.(印刷中).

Nikata., T.、Sakai, Y.、Shibata, K.、Kato, J.、Kuroda, A. 和 Ohtake, H.：“铜绿假单胞菌 Mol 磷酸盐特异性转运 (pst) 操纵子的分子分析。” .Gen.Gent.（正在出版）。

Kato, J., Sakai, Y.Nikata, T., and Ohtaka, H.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis." J.Bacteriol.176. 5874-5877 (1994)

Kato, J.、Sakai, Y.Nikata, T. 和 Ohtaka, H.：“参与磷酸盐趋向性负调节的铜绿假单胞菌基因的克隆和表征。”

A.MASDUKi: "Isolation and characterization of chemotaxis mutants and genes of pseudomonas aeruginosa" J.Bacteriology. (印刷中).

A.MASDUKi：“铜绿假单胞菌趋化突变体和基因的分离和表征”J.Bacteriology（正在出版）。

Kuroda, A., Kumano, T., Taguchi, K., Nikata, T., Kato, J.and Ohtake, H.: "Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa." J.Bacteriol.177. 7019-7125 (1995)

Kuroda, A.、Kumano, T.、Taguchi, K.、Nikata, T.、Kato, J. 和 Ohtake, H.：“铜绿假单胞菌趋化转导基因的分子克隆和表征。”

Ikegami,S.,Imai.I.,Kato,J.and Ohtake,H.: "Chemotaxis toward inorganic phosphate in the red tide alga Chattonella antigua." J.Plankton Res.,. 17. 1586-1590 (1995)

Ikegami,S.、Imai.I.、Kato,J. 和 Ohtake,H.：“赤潮藻安提瓜查托氏菌对无机磷酸盐的趋化性”。