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Molecular genetics on muscle protein metabolism of domestic animal

家畜肌肉蛋白代谢的分子遗传学

基本信息

批准号：
10460124
负责人：
MAEDA Yoshizane
金额：
$ 8.58万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460124/
关键词：
protein turnover rate skeletal muscle calpain calpastatin FISH RFLP 筋肉蛋白質

项目摘要

This studies were conducted to clarify 1) genetic control of muscle protein turnover rate and calpain-calpastatin activity of poultry, 2) cloning of calpain and calpastatin genes, 3) expression of calpain and calpastatin genes, and 4) mapping of calpain gene.It was recognized that muscle protein turnover rates differed among breeds and strains, and highly correlated with calpain and calpastatin activities in skeletal muscle of chicken and quail. In the fattening stars of Japanese Black Cattle, the amount of excretion of methylhistidine was differed among sire families. This results suggest that the muscle protein turn over rate are differ among sire families. The cDNA of calpain from muscle of quail was cloned and sequenced (2112bp). The cDNA was composed of 4 domeins like chicken. The identity of quail calpain sequence was 96.8%, 71.4%, 71.3% and 71.6% with chicken, human mouse and cattle., respectively. Although degree of expression of calpain gene in skeletal muscle was differed among quail lines selected for body size, the structure of regulation region of calpain gene were no difference among quail lines. These results suggest that difference in gene expression among quail lines may be caused by hormonal factors. By FISH method, calpain cDNA were mapped on the chromosome No.3. In this research, we could not amplify calpastatin fragment from cDNA of quail muscle.

本研究旨在阐明1）家禽肌肉蛋白质周转率和calpain-calpastatin活性的遗传控制，2）calpain和calpastatin基因的克隆，3）calpain和calpastatin基因的表达，4）calpain基因的定位。与鸡和鹌鹑骨骼肌钙蛋白酶和钙蛋白酶抑制剂活性高度相关。在日本黑牛育肥过程中，不同父系家系的甲基组氨酸排泄量不同。这一结果表明，不同父系的肌肉蛋白质转化率存在差异。从鹌鹑肌肉中克隆了钙蛋白酶的cDNA并进行了序列测定（2112 bp）。该cDNA由4个domeins组成，与鸡相似。鹌鹑钙蛋白酶序列与鸡、人鼠和牛的同源性分别为96.8%、71.4%、71.3%和71.6%。分别不同体尺鹌鹑骨骼肌钙蛋白酶基因表达水平不同，但钙蛋白酶基因调控区结构无明显差异。这些结果表明，鹌鹑品系间基因表达的差异可能是由激素因素引起的。通过FISH技术将calpain cDNA定位于第3号染色体上。本研究未能从鹌鹑肌肉cDNA中扩增出calpastatin片段。