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Moleculor analysis on prion gene of Bos

Bos朊病毒基因的分子分析

基本信息

批准号：
16580225
负责人：
MAEDA Yoshizane
金额：
$ 2.43万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580225/
关键词：
BSE Prion Gene Native cattle プリオンアジア在来牛多様性

项目摘要

To date, many polymorphisms have been identified in the bovine prion protein gene (PRNP). Recently, Sander et al. (2004) reported significant associations between BSE susceptibility in German cattle and the frequency distributions of 23bp insertion/deletion (indel) polymorphisms within the putative bovine PRNP promoter. In this study, we investigated the frequency distribution of the 23bp indel polymorphism for 324 animals from five Asian native cattle populations and four animals from Mithan (Bos frontalis) and compared them to those described by Sander et al. (2004). The results revealed the following :1)The 23bp indel region was polymorphic in all populations examined in this study. The frequency of 23bp (+) allele was higher than that of 23bp (-) allele in all populations except Mithan.2)Significant differences (P < 0.05 in all tests) were detected between the frequencies of 23bp allele for Vietnam north native cattle and Mithan, and BSE-affected German cattle described by Sander et al. (2004).3)The frequencies of the 23bp promoter alleles observed for Mongol, Vietnam south, and Myanmar native cattle resembled that of BSE-affected German cattle. Significant differences (P < 0.05 in all tests) were detected between the frequencies of 23bp allele for Vietnam south and Myanmar native cattle, and healthy German cattle.

迄今为止，在牛朊蛋白基因（PRNP）中已经发现了许多多态性。最近，Sander等人（2004）报道了德国牛的疯牛病易感性与牛PRNP启动子中23bp插入/缺失（indel）多态性的频率分布之间的显著关联。在这项研究中，我们调查了来自5个亚洲本地牛种群的324只动物和4只来自米桑的动物（bosfrontalis）的23bp indel多态性的频率分布，并将其与Sander et al.（2004）的描述进行了比较。结果表明：1)在所有群体中，23bp的indel区均存在多态性。除米森牛外，其余人群23bp（+）等位基因频率均高于23bp（-）等位基因频率。2)越南北部本地牛和米森牛的23bp等位基因频率与Sander et al.（2004）描述的感染疯牛病的德国牛的23bp等位基因频率之间存在显著差异（所有试验均P < 0.05）。3)蒙古、越南南部和缅甸本地牛的23bp启动子等位基因频率与感染疯牛病的德国牛相似。越南南部和缅甸本地牛的23bp等位基因频率与健康德国牛的23bp等位基因频率差异显著（P < 0.05）。