ANALYSIS OF CHARACTERISTICS AND PATHOGENESIS OF SKIN INJURY INDUCED BY ENVIRONMENTAL CHEMICALS AND ULTRAVIOLET LIGHT

环境化学物质和紫外线所致皮肤损伤的特点及发病机制分析

• 批准号: 10460139
• 负责人: DOI Kunio
• 金额: $ 7.23万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460139/
• 关键词: Environmental chemicals; Ultraviolet light; Skin injury; Apoptosis; Hairless dogs; Hairless rats; WBN; ILA-Ht rat; T-2 toxin; basal cell apoptosis; IQI; Jic mouse; picryl chloride; contact dermatitis; UV irradiation; Haieless dog; Skin res

Skin reaction to ultraviolet light irradiation was examined using hairless dogs and rats. Erythema, sunburn and suntan reactions were observed as in humans. Histopathological examination for the skin revealed apoptotic cell death in the epidermal lesions. Involvement of p53 and p21 genes in the induction of apoptosis was suggested. Skin lesions were also induced by the application of an agricultural chemical, Maneb. The lesions were enhanced by ultraviolet irradiation in dogs, but not in rats. There may be a species differences in the skin reaction.Skin reaction to topical application of formaldehyde and hydrogen peroxide was clarified using hairless rats. Skin application of a mycotoxin, T-2 toxin, was additionally performed. The results indicated apoptotic cell death of epidermal cells and endothelial injury in the dermis.The results of the research clarified characteristics and pathogenesis of the skin injury induced by environmental chemicals and ultraviolet light.

用无毛狗和大鼠检查皮肤对紫外线照射的反应。观察到与人体相同的红斑、晒伤和晒黑反应。皮肤组织学检查显示表皮病变中的细胞凋亡。p53和p21基因参与诱导细胞凋亡。施用农业化学品Maneb也会引起皮肤损伤。紫外线照射可使狗的病变增强，但对大鼠无此作用。皮肤反应可能存在种属差异。使用无毛大鼠阐明了局部应用甲醛和过氧化氢的皮肤反应。另外进行真菌毒素T-2毒素的皮肤应用。结果表明，皮肤表皮细胞凋亡，真皮内皮细胞损伤，阐明了环境化学物质和紫外线引起皮肤损伤的特点和发病机制。

项目成果

Ikeda M., Kuroki K., Suzuki H., Nakayama H., Saegusa J.and Doi K.: "Picryl chloride-induced allergic dermatitis in IQI/Jic female mice."Exp.Toxicol.Pathol.. 52. 235-240 (2000)

Ikeda M.、Kuroki K.、Suzuki H.、Nakayama H.、Saegusa J. 和 Doi K.：“三氯丙烯在 IQI/Jic 雌性小鼠中诱发过敏性皮炎。”Exp.Toxicol.Pathol.. 52. 235-

Iwamoto S.,Nakayama H.,Dot K.: "Morphological and morphometrical study on the dorsal skin of Wistar and WBN/ILA-Ht rats in their developing stage.Evaluation of the proliferation and ......."Histology and Histopathology. 13. 981-988 (1998)

Iwamoto S.、Nakayama H.、Dot K.：“Wistar 和 WBN/ILA-Ht 大鼠发育阶段背部皮肤的形态学和形态测量研究。增殖评估和……”组织学和

Albarenque S.M. et al.: "Kinetics and distribution of transforming growth factor (TGF)-β1 mRNA in the dorsal skin of hypotrichotic WBN/ILA-Ht rats following topical applicayion of T-2 toxin"Exp Toxicol Pathol. 52. 29-301 (2000)

Albarenque S.M. 等人：“局部应用 T-2 毒素后，少毛 WBN/ILA-Ht 大鼠背部皮肤中转化生长因子 (TGF)-β1 mRNA 的动力学和分布”Exp Toxicol Pathol。 (2000)

Kimura T.and Doi K.: "Dorsal skin reactions of hairless dogs to topical treatment with corticosteroids."Toxicologic Pathology. 27. 528-535 (1999)

Kimura T. 和 Doi K.：“无毛狗对皮质类固醇局部治疗的背部皮肤反应。”毒理学病理学。

Ishii Y.,Tsutsui K.,Doi K.,Itagaki S.: "Hair follicles of young Wistar strain hairless rats : a histological study."Journal of Anatomy. 191. 99-106 (1997)

Ishii Y.、Tsutsui K.、Doi K.、Itagaki S.：“年轻 Wistar 品系无毛大鼠的毛囊：组织学研究。”解剖学杂志。