The role of DIG-1 gene steroid-induced apoptosis.

DIG-1基因类固醇诱导细胞凋亡的作用。

• 批准号: 10470053
• 负责人: UEDE Toshimitsu
• 金额: $ 7.49万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470053/
• 关键词: steroid; apoptosis; thymocytes; transgenic mice; アネキシンV; 分化

1. We cloned and sequence a DIG-1 gene that specifically expressed by thymocytes after dexamethason(DEX)treatment.2. DIG-1 gene encodes 7 transmembrane G protein coupled receptor.3. We have established 3 lines of DIG-1 transgenic mice.4. In transgenic mice, thymus were significantly smaller than those in control mice. After DEX injection, the apoptotic cells within thymic cortex increased siginificantly as compared to those in control mice. The apoptotic cells were mainly CD4+CD8+ thymocytes.5. The expression of apoptosis-related proteins, Bcl-2 and BAX were not significantly different from thosse in transgenic mice.

1.我们克隆并测序了地塞米松（DEX）处理后胸腺细胞特异性表达的DIG-1基因. DIG-1基因编码7个跨膜G蛋白偶联受体.建立了3个DIG-1转基因小鼠品系.转基因小鼠胸腺明显小于对照小鼠。DEX注射后，胸腺皮质内凋亡细胞明显增多。凋亡细胞主要为CD 4 + CD 8+胸腺细胞.凋亡相关蛋白Bcl-2和BAX的表达与转基因小鼠无明显差异。

项目成果

M.Takiguchi: "Involvement of CD28/CTLA4-B7 costimulatory pathway in the development of lymphadenopathy and splenomegaly in MRL/1pr mice."J.Vet.Med.Sci.. 62(1). 29-36 (2000)

M.Takiguchi：“CD28/CTLA4-B7 共刺激途径参与 MRL/1pr 小鼠淋巴结肿大和脾肿大的发生。”J.Vet.Med.Sci. 62(1)。

S.Kon: "Antibodies to different peptides in osteopontin reveal complexities in the various secreted forms."J.Cell.Biochem.. 77(3). 487-498 (2000)

S.Kon：“骨桥蛋白中不同肽的抗体揭示了各种分泌形式的复杂性。”J.Cell.Biochem.. 77(3)。

H.Harada, H.Ishikura, I.Nakagawa, J.Shindo, M.Murakami, T.Uede, T.Koyanagi: "Abortive alloantigen presentation by donor dendritic cells leads to donor-specific tolerance : a study with a preoperative CTLA4Ig inoculation."urol.Res.. 28(1). 69-74 (2000)

H.Harada、H.Ishikura、I.Nakakawa、J.Shindo、M.Murakami、T.Uede、T.Koyanagi：“供体树突状细胞的流产同种抗原呈递导致供体特异性耐受：一项术前 CTLA4Ig 接种的研究

M.Takiguchi, M.Murakami, I.Nakagawa, I.Saito, A.Hashimoto, T.Uede: "CTLA4IgG gene delivery prevents autoantibody production and lupus nephritis in MRL/lpr mice."Life Sci.. 66(11). 991-1001 (2000)

M.Takiguchi、M.Murakami、I.Nakakawa、I.Saito、A.Hashimoto、T.Uede：“CTLA4IgG 基因传递可预防 MRL/lpr 小鼠的自身抗体产生和狼疮性肾炎。”Life Sci.. 66(11)。

S.Chikuma, M.Murakami, K.Tanaka, T.Uede: "Janus kinase 2 is associated with a box 1-like motif and phosphorylates a critical tyrosine residue in the cytoplasmic region of cytotoxic T lymphocyte associated molecule-4."J.Cell.Biochem.. 78(2). 241-250 (2000)

S.Chikuma、M.Murakami、K.Tanaka、T.Uede：“Janus 激酶 2 与类似 box 1 的基序相关，并磷酸化细胞毒性 T 淋巴细胞相关分子 4 细胞质区域中的关键酪氨酸残基。”J