Analysis of mechanism that control B cell activation by using monoclonal antibodies.

我们已经生产了一组单克隆抗体，用于检测人类淋巴样细胞的不同细胞表面抗原系统。首先，利用BL1-4D6和TB3-7D5单克隆抗体，鉴定了在两个不同的人B细胞亚群上表达的两个抗原系统（l29和l30）。表达l29的B细胞是位于淋巴滤泡生发中心的大型活化B细胞。相反，表达l30的B细胞是位于淋巴滤泡套带的静止B细胞。l30阳性的B细胞在细胞表面也表达IgM和IgD。有丝分裂原刺激外周血淋巴细胞后，l30表达减弱，而l29表达增强，并伴有IL - 2受体和T10抗原的表达。因此，l30定义试验b细胞，而l29定义活化b细胞。我们还制备了一种检测白细胞介素2受体的单克隆抗体（l10）。其次，这些单克隆抗体用于恶性淋巴瘤和白血病的鉴别诊断。毛细胞白血病表达l29和l30aw以及IL-2受体（l10）。大多数弥漫性小裂细胞淋巴瘤表达l30和l10，而不表达l29抗原。大细胞淋巴瘤和免疫母细胞淋巴瘤表达l29抗原，而不表达l30抗原。第三，我们已经生成了一种单克隆抗体，它与白细胞的一种独特的抗原决定因子-共同抗原（LCA）反应。已知LCA在T细胞、B细胞、巨噬细胞和粒细胞等多种细胞中表达。单克隆抗体Y1仅与Jurkat细胞反应。可能LCA在细胞转化过程中发生了抗原修饰。值得注意的是，部分ATL细胞也表达Y1抗原。因此，共同的细胞表面抗原可能表达一种独特的抗原决定因素，这种独特的抗原可能作为肿瘤特异性标记物。

We have produced a battery of monoclonal antidobies that detect different cell-surface antigen systems of human lymphoid cells. Firstly, two antigen systems (L 29 and L 30) expressed on two distinct human B cell subpopulations were identified by using BL1-4D6 and TB3-7D5 monoclonal antibodies. B cells that express L 29 were large activated B cells located in the germinal center of lymphoid follicles. In contrast, B cell that express L 30 were resting B cells located in the mantle zone of lymphid follicles. Those L 30 positive B cells also expressed IgM and IgD on their cell surgace. Upon stimulation of peripheral blood lymphocytes by mitogen, the expression of L 30 became weak whereas the expression of L 29 became strong which was accompanied by IL 2 receptor and T10 antigen expression. Therefore,L 30 defines festing b cells whereas L 29 defines activated B cells. We have also generated a monoclonal antibody (L 10) that detect interleukin 2 receptor.Secondly, those monoclonal antibodies were used for the differential diagnosis of malignant lymbhomas and leukemias. Hairly cell leukemias express L 29 and L 30 aw well as IL-2 receptor (L 10). MOst cases of diffuse small cleaved cell lymphoma expressed L 30 and L 10 whereas they did not express L 29 antigen. Large cell and immunoblastic lymphomas express L 29 antigen, but did not express L 30 antigen.Thirdly, we have henerated a monoclonal antibody that react with an unique antigenic determinatnt of leukocyte-common antigen (LCA). LCA was known to beexpressed by variety of cells such as T cells B cells, macrophages and granulocytes. However, monoclonal antibody, Y1 only reacted with Jurkat cells. It is possible that LCA underwent antigenic modification upon trasformation of cells. It should be noted that Y1 antigen was also expressed by some ATL cells. Therefore, common cell surface antigen may express a unique antigenic determinant and this unique antigen may serve as tumor specific marker.