Molecular analysis of steroid-induced apoptosis and its relevance to disease pathogenesis.

类固醇诱导的细胞凋亡的分子分析及其与疾病发病机制的相关性。

• 批准号: 13470047
• 负责人: UEDE Toshimitsu
• 金额: $ 9.09万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470047/
• 关键词: Thymocyte; Apoptosis; Transgenic mice; steroid; caspase

1. Trangenic mice (TG) was established in which TDAG8 gene responsible for glucocorticoid-induced apoptosis was over-expressed.2. The expression of mRNA including FasL, FADD, TRAIL, TNF-R and TRADD did not differ between TG and control mice upon glucocorticoid treatment.3. TG mice exhibited augmented thymocyte apoptosis induced by glucocorticoid as compared to control mice.4. Upon glucocorticoid treatment, activity of caspase 3, 8, and 9 was significantly augmented in TG mice as compared to control mice.

1.建立糖皮质激素诱导细胞凋亡相关基因TDAG8过度表达的转基因小鼠(TG)。糖皮质激素治疗后，TRAIL、FADD、TRAIL等基因与对照组小鼠相比，糖皮质激素诱导的转基因小鼠胸腺细胞凋亡率显著增加。糖皮质激素治疗后，与对照小鼠相比，转基因小鼠caspase3、8和9的活性显著增强。

项目成果

Yamashita, K., Masunaga, T., Yanagida, N., Takehara, M., Hashimoto, T., Kobayashi, T., Echizenya, H., Hua, N., Fujita, M., Murakami, M., Furukawa, H., Uede, T., Todo, S.: "Long-term acceptance of rat cardiac allografts on the basis of adenovirus mediated

山下 K.、增永 T.、柳田 N.、竹原 M.、桥本 T.、小林 T.、越前屋 H.、华 N.、藤田 M.、村上 M.、

Kim, JH., Skates, SJ., Uede, T., Wong, Kk KK., Schorge, JO., Feltmate, CM., Berkotivitz, RS., Cramer, DW., Mok, SC.: "Osteopontin as a potential diagnostic biomarker for ovarian cancer."JAMA. 287(13). 1671-1679 (2002)

Kim, JH.、Skates, SJ.、Uede, T.、Wong, Kk KK.、Schorge, JO.、Feltmate, CM.、Berkotivitz, RS.、Cramer, DW.、Mok, SC.：“骨桥蛋白作为

Salama, AD., Yuan, X., Nayer, A., Chandraker, A., Inobe, M., Uede, T., Sayegh, MH.: "Interaction between ICOS-B7RP1 and B7-CD28 costimulatory pathways in alloimmune responses in vivo."Am J Transplant. 3(4). 390-395 (2003)

Salama, AD.、Yuan, X.、Nayer, A.、Chandraker, A.、Inobe, M.、Uede, T.、Sayegh, MH.：“ICOS-B7RP1 和 B7-CD28 共刺激途径在同种免疫反应中的相互作用

Tsuji, H.: "Adenovirus-mediated in vivo B7-1 gene transfer induces anti-tumor immunity against pre-established primary tumor and pulmonary metastasis of rat osteosarcoma"Cancer Gene Ther. 9. 745-755 (2002)

Tsuji, H.：“腺病毒介导的体内 B7-1 基因转移诱导针对大鼠骨肉瘤预先建立的原发肿瘤和肺转移的抗肿瘤免疫”Cancer Gene Ther。

Iwasaki, N.: "Feasibility of immunosuppression in composite tissue allografts bysystemic administration of CTLA4Ig"Transplantation.. 73. 334-340 (2002)

Iwasaki, N.：“通过 CTLA4Ig 全身给药复合组织同种异体移植物中免疫抑制的可行性”Transplantation.. 73. 334-340 (2002)