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The correlation between chromosome aberrations and head morphology or motility of human spermatozoa : Analysis by using intracytoplasmic sperm injection into mouse oocytes

染色体畸变与人类精子头部形态或运动力之间的相关性：通过使用胞浆内精子注射到小鼠卵母细胞中进行分析

基本信息

批准号：
10470339
负责人：
KAMIGUCHI Yujiroh
金额：
$ 5.12万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470339/
关键词：
Human spermatozoa Chromosome aberrations Mouse oocytes intracytoplasmic sperm injection

项目摘要

(1) A highly successful method for human sperm chromosome analysis was established by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Namely, 7O.7% of sperm-injected oocytes were able to reach the first cleavage metaphase, and 94.6% of metaphasic ova were successfully karyotyped.(2) In vitro aging of mouse oocytes and human spermatozoa, which takes place when they are waited for ICSI in the culture medium, may be associated with the incidence of sperm chromosome aberrations. However, no change was found in the aberration frequency even when both gametes were aged in vitro for about 3 hours.(3) In motile human spermatozoa with normal morphology, the incidences of aneuploidy and structural chromosome aberrations were 1.7% and 8.8% respectively. These incidences were not statistically different between fresh and frozen-thawed spermatozoa, indicating that cryopreservation of spermatozoa in liquid nitrogen has no deleterious effect on sperm chromosomes.(4) No increase of the chromosome aberrations was found in the spermatozoa with a large head, a small head, and a pointed head, as compared with the aberration frequency in morphologically normal spermatozoa. In contrast, the frequency was significantly higher in the spermatozoa with an elongated head.(5) The incidence of structural chromosome aberrations in immotile spermatozoa with normal morphology was similar to that in motile spermatozoa when immotile spermatozoa were kept in seminal fluid until ICSI.On the contrary, aberration frequency increased significantly when immotile spermatozoa were stored in the culture media (BWW, HTF) for 2 hours or more. An increasing tendency was found in PBS-stored spermatozoa. Aberration frequency was also heightened by centrifugation of spermatozoa.

(1)采用小鼠卵母细胞胞浆内单精子注射（ICSI）技术，建立了一种成功的人精子染色体分析方法。即70.7%的精子注射卵母细胞能够达到第一次分裂中期，94.6%的间相卵成功地进行了核型分析。(2)小鼠卵母细胞和人类精子在体外培养液中等待ICSI时发生的老化可能与精子染色体畸变的发生有关。然而，没有发现的畸变频率的变化，即使当两个配子在体外老化约3小时。(3)在形态正常的活动精子中，非整倍体和结构染色体畸变的发生率分别为1.7%和8.8%。这些发生率在新鲜和冷冻-解冻精子之间没有统计学差异，表明液氮中冷冻保存精子对精子染色体没有有害影响。(4)与形态正常的精子相比，大头、小头和尖头精子的染色体畸变率并未增加。与此相反，频率显着较高的精子与一个细长的头部。(5)形态正常的不动精子在精液中保存至ICSI时，其染色体结构畸变率与活动精子相似，而在培养液（BWW、HTF）中保存2 h或更长时间后，其畸变率明显增加。PBS保存的精子有增加的趋势。精子离心也可提高畸变率。