Effects of neutrons on human sperm chromosomes

中子对人类精子染色体的影响

• 批准号: 05454606
• 负责人: KAMIGUCHI Yujiroh
• 金额: $ 3.33万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454606/
• 关键词: Human sperm chromosomes; ^<252>Cf neutrons; ^<60>Co gamma-rays; Chromosome aberrations; Frozen hamster oocytes; Cryopreservation of semen; ヒト精子; 中性子; γ線; 精子凍結保存; 中性子線

Effects of ^<60>Co gamma-rays and ^<252>Cf neutrons on human sperm chromosomes were studies using our interspecific in vitro fertilization system with golden hamster oocytes to estimate relative biological effectiveness (RBE) of neutrons. The RBEs were estimated to be 1.7 and 2.0 with two indices, i.e., the frequency of chromosomally abnormal spermatozoa and the number of structural chromosome aberrations per spermatozoon. These values were considerably lower than those obtained by previous investigators in human lymphocytes (10-80), mouse spermatogonia (20-25) and early embryos (4.7-7.4).An attempt was made to apply cryopreserved golden hamster oocytes to cytogenetic study of irradiated human spermatozo. There was no significant difference in the frequency of radiation-induced sperm chromosome aberrations between the two assay systems using frozen-thawed oocytes and fresh oocytes, indicating that freezing of the oocytes does not affect the aberration frequency. Cryopreserved hamster o … More ocytes are useful to carry out spermchromosome study more conveniently and efficiently.We also tried to use cryopreserved human spermatozoa in this study. The irradiated semen samples were frozen with a simplified method, thawed at different intervals after cryopreservation, and processed for chromosome analysis. No increase of chromosome aberrations was found at any intervals, indicating that cryopreservation has no effects on radiation-induced DNA damage in spermatozoa. This method is useful for repeated use of subdivided frozen samples and transportation of sample for later cytogenetical use.To obtain background data for genetic risk assessment of radiation, spontaneous incidence of chromosome aberrations in human spermatozoa was estimated with semen samples from 51 healthy Japanese men. The frequency of chromosmally abnormal spermatozoa was 15.5(]SY.+-。[)4.2% on an average. There was no correlation between aberration frequency and age of donors. The habit of cigarette smoking was also unrelated. Less

利用金仓鼠卵母细胞的种间体外受精系统，研究了^<60>Co γ射线和^<252>Cf中子对人类精子染色体的影响，以估计中子的相对生物有效性。以染色体异常精子出现频率和每精子结构染色体畸变数为指标，估计RBEs分别为1.7和2.0。这些数值明显低于先前研究人员在人类淋巴细胞（10-80）、小鼠精原细胞（20-25）和早期胚胎（4.7-7.4）中获得的结果。尝试将冷冻保存的金仓鼠卵母细胞应用于辐照人精子的细胞遗传学研究。使用冷冻解冻卵母细胞和新鲜卵母细胞的两种检测系统在辐射诱导精子染色体畸变的频率上没有显著差异，表明卵母细胞的冷冻不影响畸变频率。冷冻保存的仓鼠细胞数量多，可方便、高效地开展精染色体研究。我们还尝试在本研究中使用冷冻保存的人类精子。经辐照后的精液标本采用简化冷冻法，冷冻保存后不同时间解冻，并进行染色体分析。在任何时间间隔内，染色体畸变均未增加，表明冷冻保存对辐射诱导的精子DNA损伤没有影响。该方法适用于重复使用细分的冷冻样品和样品的运输，以供以后的细胞遗传学使用。为了获得辐射遗传风险评估的背景数据，用51名日本健康男性的精液样本估计了人类精子中染色体畸变的自发发生率。染色体异常精子的发生率平均为15.5（±±）4.2%。畸变频率与献血者年龄无相关性。吸烟的习惯也与此无关。少

项目成果

Kamiguchi, Y.: "Chromosome analysis of gametes" Obstetrical and Gynecological Practice. 43. 255-258 (1994)

Kamiguchi, Y.：“配子染色体分析”妇产科实践。

Kamiguchi,Y.: "Chromosome abnormality in human gametes by exposure to mutagens" Environmental Mutagen Research Communications. (印刷中). (1996)

Kamiguchi, Y.：“暴露于诱变剂导致人类配子染色体异常”环境诱变剂研究通讯（1996 年出版）。

Tateno,H.: "Application of cryopreserved golden hanster oocytes to in vitro genotoxicity assays for human sperm chromosomes" Environmental and Molecular Mutagenesis. 25. 263-265 (1995)

Tateno,H.：“冷冻保存的金鸡卵母细胞在人类精子染色体体外遗传毒性测定中的应用”环境和分子诱变。

美甘和哉: "全身被曝におけるバイオドシメトリ9.ヒト精子染色体によるバイオドシメトリ" Radioisotopes. 44. 897-909 (1995)

Kazuya Mika：“全身辐射暴露中的生物剂量测定法 9。使用人类精子染色体的生物剂量测定法”放射性同位素 44. 897-909 (1995)。

Mikamo, K.: "Biodosimetry for whole body exposure. 9.Biodosimetry by human sperm chromosomes" RADIOISOTOPES. 44. 897-909 (1995)

Mikamo, K.：“全身暴露的生物剂量测定。9.人类精子染色体的生物剂量测定”放射性同位素。