Effects of radiation and chemicals on human sperm chromosomes

辐射和化学物质对人类精子染色体的影响

• 批准号: 02455006
• 负责人: KAMIGUCHI Yujiroh
• 金额: $ 3.39万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02455006/
• 关键词: Human sperm; Chromosome aberration; gamma-rays; antineoplastic agents; antibiotics; zona-free hamster ova; in vitro fertilization; 透明帯除去ハムスタ-卵

The chromosome constitution of human spermatozoa was studied using our interspecific in vitro fertilization system between human sperm and zona- free hamster oocytes. The results are as follows:1.Effects of gamma-rays: Total numbers of 6,977 and 6,932 sperm were analyzed in the control and irradiated groups. Within a dose range from 6.4 to 109.3 cGy, the incidence of sperm with gamma-ray-induced structural chromosome aberrations increased linearly with increasing dosage. The incidence of breakage-type aderations was far higher than that of exchange- type aberrations. The dose-dependent increase was linear in the former, whereas it was quadratic in the latter.2.Effects of antineoplastic agents: Total numbers of 728 and 650 sperm were analyzed in the control and chemical-treated groups. The incidence of sperm with structural chromosome aberrations were 24.3%,47.3%,54.1% and 50.7% in bleomycin (50 ug/ml, 90 min), daunomycin (1 ug/m, 90 min),methyl methanesulfonate (100 ug/ml, 120 min), tr … More iethylenemelamine (0.1 ug/ml, 120 min) groups respectively, showing significantly higher incidences than those in matched controls. No significant in crease was found in cyclophosphamide 1-1000 ug/ml, 120 min) and mitomycin C (0.1-100 ug/ml, 120 min) groups.3.Spontaneous incidence of chromosome aberrations in human sperm: A total of 4,579 sperm from 22 men were analyzed. The mean incidences of aneuploidy and structural anomaly were 1.3/ and 15.1%. The latter incidence varied considerably from donor to donor (7.0-24.8%).4.Chromosome analysis in frozen-thawed human sperm: Five different cryopreservation media were tested. Post-thaw motility of sperm was higher in KS-II medium and TEST-yolk buffer medium (50-70%) than in HSP medium, modified HSP medium and Ackerman's medium (10-40%). There was a great individual variation in survival rate of post-thaw sperm. Rates of post- thaw sperm penetration into hamster ova and subsequent sperm chromosome analysis were considerably low even in the former two media. Further improvement is necessary. Less

利用我们建立的仓鼠卵与人精子体外受精系统，研究了人精子的染色体组成。结果如下：1. γ射线的影响：对照组和照射组的精子总数分别为6，977和6，932。在6.4~109.3 cGy剂量范围内，γ射线诱导的精子染色体结构畸变的发生率随剂量的增加而线性增加。断裂型畸变的发生率远高于交换型畸变。精子数的增加在对照组和化学处理组中分别为728和650个精子。博莱霉素（50 μ g/ml，90 min）、柔红霉素（1 μ g/ml，90 min）、甲磺酸甲酯（100 μ g/ml，120 min）、红霉素（100 μ g/ml，120 min）的精子染色体畸变率分别为24.3%、47.3%、54.1%和50.7 ...更多信息 乙烯三聚氰胺（0.1 μ g/ml，120分钟）组的发生率明显高于配对对照组。环磷酰胺（1 - 1000 μ g/ml，120 min）和丝裂霉素C（0.1 - 100 μ g/ml，120 min）组精子染色体畸变率无明显增加。非整倍体和结构异常的平均发生率分别为1.3%和15.1%。后者的发生率在不同供体之间有很大差异（7.0 - 24.8%）。4.人精子冷冻解冻后的染色体分析：对五种不同的冷冻保存液进行了试验。精子解冻后活力在KS-Ⅱ和TEST-卵黄缓冲液中为50 - 70%，在HSP、改良HSP和阿克曼氏液中为10 - 40%。解冻后精子存活率存在较大的个体差异。即使在前两种培养液中，解冻后精子穿透仓鼠卵的比率和随后的精子染色体分析也相当低。需要进一步改进。少

项目成果

Kamiguchi,Y: "Radiosensitivity in human sperm chromosomes. In "A View for Utilization of Radiation in Elucidation of Biological Phenomena", Ikushima,R.(Ed),Research Reactor Institute,Kyoto Univ.,Kumatori'" 57-68 (1991)

Kamiguchi,Y：“人类精子染色体中的放射敏感性。在“利用辐射阐明生物现象的观点”中，Ikushima,R.（编辑），研究堆研究所，京都大学，Kumatori”57-68（1991）

Mikamo,K.et al: "The interspecific in vitro fertilization system to measure human sperm chromosomal damage. In "New Horizons in Biological Dosimetry",Gledhill,B.L.and Mauro,F.(Eds.),Wiley-Liss,Inc.,New York" 531-542 (1991)

Mikamo,K. 等人：“用于测量人类精子染色体损伤的种间体外受精系统。在“生物剂量测定的新视野”中，Gledhill,B.L. 和 Mauro,F.（编辑），Wiley-Liss,Inc.，

Kamiguchi,Y.,Tateno,H.and Mikamo,K.: "Micronucleus test in 2-cell embryos as a simple assay system for human sperm chromosome aberrations." Mutation Research. 252. 297-303 (1991)

Kamiguchi,Y.、Tateno,H. 和 Mikamo,K.：“2 细胞胚胎中的微核测试作为人类精子染色体畸变的简单检测系统。”

Mikamo,K.,Kamiguchi,Y.and Tateno,H.: "Proceedings of Second International Symposium on Chromosome Aberration." Obe,G.and Natarajan,A.T.(Eds.),

Mikamo,K.、Kamiguchi,Y. 和 Tateno,H.：“第二届染色体畸变国际研讨会论文集”。

Kamiguchi,Y.et al: "Micronucleus test in 2-cell embryos as a simple assay system for human sperm chromosome aberrations." Mutation Res. 252. 297-303 (1991)

Kamiguchi,Y.et al：“2 细胞胚胎中的微核测试作为人类精子染色体畸变的简单检测系统。”