Studies on ontogenic significance of PLC signaling systems.

PLC信号系统的本体意义研究。

基本信息

  • 批准号:
    10480172
  • 负责人:
  • 金额:
    $ 7.36万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 2000
  • 项目状态:
    已结题

项目摘要

Phospholipase C (PLC) hydrolyzes phosphatidylinositol-4,5-bisphosphate (PIP_2) to generate inositol-1,4,5-trisphosphate (IP_3) and diacylglycerol (DAG), and this catalytic activity is controlled upon receptor activation. It is well understood that these two products, IP_3 and DAG, mediate the calcium release from intracellular stores and protein kinase C activation. Among PLC isoforms, this study focused on biological function of PLC-γ2, Mice with loss of PLC-γ2 function were produced using two different gene knockout strategies. Following results were obtained from this research project.1) Using gene-targeting approach, mice heterogeneously harboring PLC-γ2 gene of which a catalytic X region was replaced by neo, were successfully produced. The homozygous mice, however, were embryonic lethal and died at 〜E8.5.2) The PLC-γ2 gene was inducibly ablated by using IFN-regulated Cre recombinase. Mice with neonatally induced loss of PLC-γ2 function displayed reduced members of mature conventional B cells and peritoneal B l cells and defective responses in vitro to BCR stimulation and in vivo to immunization with thymusindependent type-II Ags. In contrast, T cell development and TCR-mediated proliferation were normal. These results indicate that PLC-γ2 is a critical component of BCR signaling pathways and is required to promote B cell development.
磷脂酶C(PLC)催化磷脂酰肌醇-4,5-二磷酸(PIP_2)生成肌醇-1,4,5-三磷酸(IP_3)和二酰甘油(DAG),这种催化活性受受体激活的控制。众所周知,这两个产物,IP_3和DAG,介导细胞内钙释放和蛋白激酶C的激活。在PLC亚型中,本研究以PLC-γ2的生物学功能为研究重点,采用两种不同的基因敲除策略建立了PLC-γ2功能缺失的小鼠模型。1)利用基因打靶的方法,成功地获得了异源携带pLc-γ-2基因的小鼠,其催化X区被NEO取代。然而,纯合子小鼠是胚胎致死的,并在E8.5岁时死亡。5)用干扰素调节的Cre重组酶诱导PLC-γ2基因被烧毁。新生鼠PLC-γ-2功能丧失后,成熟的常规B细胞和腹膜B-L细胞成员减少,体外对bcr刺激和体内对胸腺非依赖性II型AGS免疫的反应有缺陷。相比之下,T细胞发育和TCR介导的增殖是正常的。这些结果表明,PLC-γ2是bcr信号通路的重要组成部分,是促进B细胞发育所必需的。

项目成果

期刊论文数量(54)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
ITO Taiji, KABUYMA Yukihito, OKAZAKI Satoshi, KAMEDA Takashi, MURAKAMI Masao, IBA Hideo: "Unstable retrovirus mutants with acquired transforming activity: rapid changes in the number of repeats of a specific junD polynucleotide segments"Nucleic Acids Res.
ITO Taiji、KABUYMA Yukihito、OKAZAKI Satoshi、KAMEDA Takashi、MURAKAMI Masao、IBA Hideo:“具有获得性转化活性的不稳定逆转录病毒突变体:特定junD多核苷酸片段重复次数的快速变化”核酸研究。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Satoh,K., et al.: "Involvement of ErbB-2 in rheumatoid synovial cell growth."Arthritis Rheum.. (in press).
Satoh,K., et al.:“ErbB-2 在类风湿滑膜细胞生长中的参与。”关节炎大黄..(出版中)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Horiguchi,H., et al.: "Cadmium-induced acute hepatic injury is exacerbated in interleukin-8 transgenic mice."Toxicol.Appl.Pharmacol.. 163. 231-239 (2000)
Horiguchi, H., et al.:“镉诱导的急性肝损伤在白细胞介素 8 转基因小鼠中加剧。”Toxicol.Appl.Pharmacol.. 163. 231-239 (2000)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
A.Hashimoto, et al.: "Essential role of phospholipase C-g2 in B cell development and function"J.Immunol.. 165. 1738-1742 (2000)
A.Hashimoto 等:“磷脂酶 C-g2 在 B 细胞发育和功能中的重要作用”J.Immunol.. 165. 1738-1742 (2000)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
N.Oyama, et al.: "Different growth properties in response to EGF and IL-6 of primary keratinocytes derived from normal-"J.Dermatol.Sci.. 16. 120-128 (1998)
N.Oyama 等人:“源自正常的原代角质形成细胞对 EGF 和 IL-6 的不同生长特性”J.Dermatol.Sci.. 16. 120-128 (1998)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

HOMMA Yoshimi其他文献

HOMMA Yoshimi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('HOMMA Yoshimi', 18)}}的其他基金

Studies on molecular basis for regulation of beta-oxidation system
β-氧化系统调控的分子基础研究
  • 批准号:
    21590314
  • 财政年份:
    2009
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of DNA methylation pattern of promoter CpG islands in idiopathic pulmonary fibrosis.
特发性肺纤维化启动子CpG岛DNA甲基化模式分析
  • 批准号:
    15390257
  • 财政年份:
    2003
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Cooperative study on molecular mechanism of GFAP expression.
GFAP表达分子机制合作研究。
  • 批准号:
    10044307
  • 财政年份:
    1998
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification and functional analysis of proteins involved in novel pathway for PLC activation.
PLC 激活新途径中涉及的蛋白质的鉴定和功能分析。
  • 批准号:
    08458184
  • 财政年份:
    1996
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Trials for development of novel inhibitors for intracellular signaling.
开发新型细胞内信号传导抑制剂的试验。
  • 批准号:
    06558095
  • 财政年份:
    1994
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)

相似海外基金

Membrane Transport Mechanism of Aluminum and its Effect on the Inositol Phospholipid Metabolism in the Neuronal System
铝的膜转运机制及其对神经系统肌醇磷脂代谢的影响
  • 批准号:
    15590081
  • 财政年份:
    2003
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
PIP PHOSPHATASE AND INOSITOL PHOSPHOLIPID HOMEOSTATSIS
PIP 磷酸酶和肌醇磷脂稳态
  • 批准号:
    6240981
  • 财政年份:
    1996
  • 资助金额:
    $ 7.36万
  • 项目类别:
Regulation of inositol phospholipid-pelated 2nd messengers
肌醇磷脂包裹的第二信使的调节
  • 批准号:
    07044216
  • 财政年份:
    1995
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Role of tyrosine Kinase in platelet inositol phospholipid metabolism
酪氨酸激酶在血小板肌醇磷脂代谢中的作用
  • 批准号:
    03671182
  • 财政年份:
    1991
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
"Study on physical properties of inositol phospholipid in cellular signal transduction"
《肌醇磷脂在细胞信号转导中的物理性质研究》
  • 批准号:
    01580262
  • 财政年份:
    1989
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Inositol phospholipid metabolism in cerebral ischemia
脑缺血时肌醇磷脂代谢
  • 批准号:
    62480303
  • 财政年份:
    1987
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Mechanism and role of inositol phospholipid turnover in yeast morphogenesis
肌醇磷脂周转在酵母形态发生中的机制和作用
  • 批准号:
    60560090
  • 财政年份:
    1985
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
ROLE OF G-PROTEINS IN THE CONTROL OF INOSITOL PHOSPHOLIPID HYDROLYSIS IN T-CELLS
G 蛋白在控制 T 细胞中肌醇磷脂水解中的作用
  • 批准号:
    3811105
  • 财政年份:
  • 资助金额:
    $ 7.36万
  • 项目类别:
PIP PHOSPHATASE AND INOSITOL PHOSPHOLIPID HOMEOSTATSIS
PIP 磷酸酶和肌醇磷脂稳态
  • 批准号:
    5212607
  • 财政年份:
  • 资助金额:
    $ 7.36万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了