Physiological basis of the regulation of seedling emergence of rice plant

水稻出苗调控的生理基础

• 批准号: 10556004
• 负责人: AKITA Shigemi
• 金额: $ 8.06万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10556004/
• 关键词: amylase; seedling emergence; germination; coleoptile; rice plant; direct seeding; expansin

Traits related with the seedling emergence under flooded condition which is required for the stabilized direct seeding of rice were studied. Isoform H of Amylase which is encoded with Ramy 3D gene was identified as one of the most limiting step for the decomposition of starch under flooded condition both by northern blotting and by in situ hybridization methods. Besides, the involvement of alchohol dehydrogenase in the regulation of seedling emergence were examined. However, the role of ADH was not always in parallel with the seeding emergence under flooded condition and the participation of other limiting steps was suggested. As the possible factors for the regulation of coleoptile elongation which is indispensable for the seedling emergence under flooded condition, the role of expansin was studied. Two isoforms of expansin was identified as the possible factors for the regulation of coleoptile length under flooded condition. Thus the participation of at least 3 major limiting steps was elucidated in the regulation process of seedling emergence of rice under flooded condition.

对水稻稳定直播所需的淹水出苗相关性状进行了研究。利用北方杂交和原位杂交技术，证实Ramy 3D基因编码的淀粉酶H亚型是淹水条件下淀粉分解的最主要限制性酶。此外，还研究了乙醇脱氢酶在幼苗出苗调控中的作用。然而，ADH的作用并不总是平行的种子在淹水条件下出现和其他限制步骤的参与。膨胀素是水稻幼苗在淹水条件下萌发所必需的胚芽鞘伸长的调控因子。膨胀素的两种异构体被认为是淹水条件下胚芽鞘长度调节的可能因子。从而阐明了至少有3个主要限制步骤参与了淹水条件下水稻出苗的调控过程。

项目成果

Huang,J.,J.Yamaguchi and S.Akita: "Ramy3D gene expression of α-amylase in rice (Oryza sativa L.) under aerobic, hypoxic and anoxic conditions"Plant Prod.Sci.. 3(in press). (2000)

Huang, J., J. Yamaguchi 和 S. Akita：“有氧、低氧和缺氧条件下水稻 (Oryza sativa L.) 中 α-淀粉酶的 Ramy3D 基因表达”Plant Sci.. 3（出版中）。 ）

Huang.J., J.Yamaguchi & S.Akita: "Changes in α-Amylase Isoforms during Emergence of Rice in Submerged Soil."Plant Prod.Sci.. 2. 12-13 (1999)

Huang.J.、J.Yamaguchi 和 S.Akita：“淹没土壤中水稻出苗期间 α-淀粉酶同工型的变化。”Plant Prod.Sci.. 2. 12-13 (1999)

Huang,J.,J.Yamaguchi & S.Akita: "Changes in α-Amylase Isoforms during Emergence of Rice in Submerged Soil."Plant Prod.Sci.. 2(1). 12-13 (1999)

Huang, J., J. Yamaguchi 和 S. Akita：“沉水土壤中水稻出苗过程中 α-淀粉酶异构体的变化。植物科学”2(1)。

Huang,J.,K.Toyofuku,J.Yamaguchi and S.Akita: "Expression of α-amylase isoforms and the RAmylA gene in rice during seed germination in submerged soil"Plant Prod.Sci.. 3(1). 32-37 (2000)

Huang, J., K. Toyofuku, J. Yamaguchi 和 S. Akita：“水稻种子萌发过程中 α-淀粉酶同种型和 RAmylA 基因的表达”《植物科学》32-。 37 (2000)

Huang,J.,K.Toyofuku,J.Yamaguchi & S.Akita: "Expression of α-Amylase Isoforms and the RAmy1A Gene in Rice (Oryza sativa L.) during Seed Germination and its Relationship with Coleoptile Length in Submerged Soil."Plant Prod.Sci.. 3(1). 32-37 (2000)

Huang, J., K. Toyofuku, J. Yamaguchi 和 S. Akita：“水稻 (Oryza sativa L.) 种子萌发过程中 α-淀粉酶同工型和 RAmy1A 基因的表达及其与沉土中胚芽鞘长度的关系。”植物生产科学 3(1)。