Eco-physhilogical basis of the alternative respiration in crops

作物替代呼吸的生态生理学基础

• 批准号: 11306002
• 负责人: AKITA Shigemi
• 金额: $ 28.47万
• 依托单位: THE UNIVERSITY OF SHIGA PREFECTURE (2001)The University of Tokyo (1999-2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11306002/
• 关键词: alternative oxydase; respiration; O18 discrimination; salt tolerance; rice plant; gene expression; mRNA; ^<18>0ディスクリミネーション

The role of alteranative respiration pathway in crop production was studies by establishing the system to measure the reaction rate based on the O187 discrimination method as well as the gene expression of alternative oxidase.The reaction rate of alternative pathway increased with growth stage indicating the significant role in crop production toward the end of growth. The significant contribution of alternative pathway on carbon economy during grain filling period in grain crops was suggeted although it is not limiting to the growth in the actively growing younger growth stages. The regulation mechanism of alternative oxidase (AOX) gene expression was elucidated. Through the compariso and protein synthesis of AOX. To know the critical role of alternative pathway on crop production, the careful examination based on O18 discrimination methd and gene expression of AOX is indispensable.

通过建立基于O187判别法的交替呼吸途径反应速率测定系统，结合交替氧化酶基因表达，研究了交替呼吸途径在作物生产中的作用，结果表明，交替呼吸途径的反应速率随生育期的延长而增加，在作物生长后期对作物生产具有重要作用。虽然替代途径对作物灌浆期碳经济的贡献并不局限于生长活跃的幼龄生长期，但它对作物灌浆期碳经济的贡献是显著的。阐明了交替氧化酶（AOX）基因表达的调控机制。通过对AOX蛋白质合成的比较.为了了解替代途径对作物生产的关键作用，基于O18鉴别方法和AOX基因表达的仔细检查是必不可少的。

项目成果

Tsuji,H.,M.Hakazono, et al.: "Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the akternative oxidase la gene."FEBS Letters. 471. 201-204 (2000)

Tsuji,H.,M.Hakazono 等人：“水稻中核编码呼吸基因的转录水平因缺氧而降低：钙参与替代氧化酶 la 基因表达的证据。”FEBS Letters。

Tsuji H., M. Nakazono, Et al.: "Transcript levels of the nuclear encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the alternative oxidase Ia gonc"FEBS Letters. 471. 201-204 (2000)

Tsuji H.、M. Nakazono 等人：“水稻中核编码呼吸基因的转录水平因缺氧而降低：钙参与替代氧化酶 Ia gonc 表达的证据”FEBS Letters。

Saisho, D., M.Nakazono, N.Tsutsumi, A.Hirai: "ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state Level of alternative oxidase mRNA in Arabidopsis thaliana"J. Plant Physiol.. 158. 241-245 (2001)

Saisho, D.、M.Nakazono、N.Tsutsumi、A.Hirai：“ATP 合成抑制剂以及呼吸抑制剂可提高拟南芥中替代氧化酶 mRNA 的稳态水平”J.

Saisho,D.,M.Nakazono,N.Tsutsumi & A.Hirai: "ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana."J.Plant Physiol.. 158. 241-245 (2001)

Saisho,D.,M.中园,N.堤

Tsuji, H., M.Nakazono, et al.: "Transcript Levels of the nuclear-encoded. respiratory genes in rice decrease by oxygen deprivation evidence for involvement of calcium in expression of the alternative oxidase la gene"FEBS Letters. 471. 201-204 (2000)

Tsuji, H., M.Nakazono 等人：“缺氧导致水稻中核编码呼吸基因的转录水平降低，证据表明钙参与了替代氧化酶 1a 基因的表达”FEBS Letters。