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Production and stabilization of antioxidant compounds in genetically modified plants introduced engineered glucosyltransferases.

转基因植物中抗氧化化合物的生产和稳定引入了工程化的葡萄糖基转移酶。

基本信息

批准号：
10557235
负责人：
YAMAZAKI Mami
金额：
$ 4.67万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Polyphenol compounds such as flavonoids, athocyanins and catecins act as strong antioxidants and prevent deseases occored by active oxygen and or other oxidants. These antioxidant polyphenols are produced in plant against environmental stresses and they have functions to reduce the oxidative stresses in plant cells. Genetically modified plants in which producibility and stability of polyphenol compounds increased are supposed to be more resistant against oxidants and oxydative stresses and can survive in arear of air pollution. On the other hand, taking high-antioxidant plants as food and medicinal resources might prevent human deseases that are induced by oxidative stresses and environmental oxidants. A group of anthocyanins are commonly produced in many plant species, and stabilized by glucosylation in their molecules in plant cells. Furthermore, anthocyanin production is coordinately regulated by transcriptional factors such as Myc and Myb homologue proteins. In this project, (1)the modification of stability and solubility of anthocyanins with engineered recombinant proteins of plant glucosyltransferases and(2)the modification of anthocyanin producibility in transgenic plant introduced Myc or Myb homologue proteins were investigated. Using several clones of glucosyltransferases from different plant species, substrate specificities were investigated. In transgenic plants introduced Myc and Myb omologues, productions of anthocyanin were increased. The details of transactivational functions of these factors were studied in vitro by using one/two hybrid systems in yeast cells.

多酚类化合物如类黄酮、花青素和儿茶素等具有很强的抗氧化作用，可防止活性氧和其它氧化剂引起的疾病。这些多酚类抗氧化剂是植物在抵抗环境胁迫时产生的，它们具有降低植物细胞中氧化胁迫的功能。转基因植物中多酚类化合物的产量和稳定性增加，被认为是更好地抵抗氧化剂和氧化胁迫，并能在空气污染地区生存。另一方面，将高抗氧化植物作为食品和药用资源，可能会预防由氧化应激和环境氧化剂引起的人类疾病。花色素苷是一组在植物中普遍存在的物质，在植物细胞中通过糖基化作用而稳定下来。此外，花青素苷的产生受转录因子如Myc和Myb同源蛋白的协同调节。本课题主要研究了（1）利用植物葡萄糖基转移酶基因工程重组蛋白对花色苷稳定性和溶解性的修饰;（2）利用Myc或Myb同源蛋白对转基因植物花色苷合成酶的修饰。使用几个克隆的葡萄糖基转移酶从不同的植物物种，底物特异性进行了研究。在转Myc和Myb基因植株中，花色素苷的产量增加。这些因子的反式激活功能的细节进行了研究，在体外通过使用一个/两个杂交系统在酵母细胞。