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Production and stabilization of antioxidant compounds in genetically modified plants introduced engineered glucosyltransferases.

转基因植物中抗氧化化合物的生产和稳定引入了工程化的葡萄糖基转移酶。

基本信息

批准号：
10557235
负责人：
YAMAZAKI Mami
金额：
$ 4.67万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Polyphenol compounds such as flavonoids, athocyanins and catecins act as strong antioxidants and prevent deseases occored by active oxygen and or other oxidants. These antioxidant polyphenols are produced in plant against environmental stresses and they have functions to reduce the oxidative stresses in plant cells. Genetically modified plants in which producibility and stability of polyphenol compounds increased are supposed to be more resistant against oxidants and oxydative stresses and can survive in arear of air pollution. On the other hand, taking high-antioxidant plants as food and medicinal resources might prevent human deseases that are induced by oxidative stresses and environmental oxidants. A group of anthocyanins are commonly produced in many plant species, and stabilized by glucosylation in their molecules in plant cells. Furthermore, anthocyanin production is coordinately regulated by transcriptional factors such as Myc and Myb homologue proteins. In this project, (1)the modification of stability and solubility of anthocyanins with engineered recombinant proteins of plant glucosyltransferases and(2)the modification of anthocyanin producibility in transgenic plant introduced Myc or Myb homologue proteins were investigated. Using several clones of glucosyltransferases from different plant species, substrate specificities were investigated. In transgenic plants introduced Myc and Myb omologues, productions of anthocyanin were increased. The details of transactivational functions of these factors were studied in vitro by using one/two hybrid systems in yeast cells.

黄酮类、花青素和儿茶素等多酚化合物可作为强抗氧化剂，预防活性氧和/或其他氧化剂引起的疾病。这些抗氧化剂多酚是植物在对抗环境胁迫时产生的，它们具有减少植物细胞氧化应激的功能。多酚化合物的产量和稳定性增加的转基因植物被认为对氧化剂和氧化应激具有更强的抵抗力，并且可以在空气污染的地区生存。另一方面，以高抗氧化植物作为食物和药用资源可能会预防由氧化应激和环境氧化剂引起的人类疾病。一组花青素通常在许多植物物种中产生，并通过植物细胞中分子的糖基化来稳定。此外，花青素的产生受到 Myc 和 Myb 同源蛋白等转录因子的协调调节。在该项目中，研究了（1）用植物葡糖基转移酶工程重组蛋白对花青素的稳定性和溶解度进行的修饰，以及（2）对引入Myc或Myb同源蛋白的转基因植物中花青素生产能力的修饰。使用来自不同植物物种的几个葡糖基转移酶克隆，研究了底物特异性。在引入Myc和Myb同系物的转基因植物中，花青素的产量增加。通过在酵母细胞中使用一/两个混合系统，在体外研究了这些因子反式激活功能的细节。