Comparative genomics of model plant and non-model plants based on metabolome analysis

基于代谢组分析的模式植物与非模式植物比较基因组学

• 批准号: 16310137
• 负责人: YAMAZAKI Mami
• 金额: $ 10.65万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310137/
• 关键词: anthocyanin; camptothecin; quinolizidine alkaloid; secondary metabolism; comparative genetics; biosynthesis; 実用植物; メタボローム; トランスクリプトー; 物質生産; GST; RNAi; 毛状根; メタボロミクス; モデル植物; フラボノイド; 生産制御; メタボロム; トランスクリプトム

In this study, the networks of gene expression and metabolism in plant were investigated by the comparative genetics between a model plant, and medicinal and/or crop plants. The chemotypes, transgenic mutants or cultured tissues that differ in secondary metabolites, were used as the experimental materials to profile genes concerning on the control mechanism of secondary metabolisms. (1) Anthocyanin production and accumulation: The specifically induced genes in PAP1-over-expressing arabidopsis were profiled and function unknown genes were obtained. By metabolome analysis of knockout lines of these genes, the function of a new BAHD acyltransferase was identified. From the comparison between GST homolgues expressed in pigmented perilla and PAP1 arabidopsis, functional difference in seed coat pimentation was clarified. (2) Camptothecin production: The candidate genes for the biosynthesitic pathway of camptothecin were profiled from the hairy roots of Ophiorrhiza pumila. The metabolome changes in RNAi hairy roots were analyzed and gene functions were predicted. The amino acid sequences of Topoisomerase I, the target protein of camptothecin, were compared among of several plants and human. The amino acid substitutions which cause the self-resistance against camptothecin were found in camptothecin-producing plants. (3) Quinolizidine alkaloid (Lupin alkaloid): Using alkaloid-containing 'Bitter form' and alkaloid-free 'Sweet form' of Lupinus angustifolius, 'bitter-specific' genes were profiled. Using the recombinant protein expressed in E. coli , enzymatic characterization of a decarboxylase was performed. Comparison of amino acid sequences of this enzyme among quinolizidine alkaloid containing species, several common substitution were found.

在这项研究中，通过模型植物与药用植物和/或农作物之间的比较遗传学来研究植物中的基因表达和代谢网络。以次生代谢物不同的化学型、转基因突变体或培养组织为实验材料，对次生代谢控制机制相关基因进行分析。 (1)花青素的产生和积累：对过表达PAP1的拟南芥中特异诱导的基因进行了分析，获得了功能未知的基因。通过对这些基因敲除系的代谢组分析，鉴定出了新的 BAHD 酰基转移酶的功能。通过比较紫苏和 PAP1 拟南芥中表达的 GST 同源物，阐明了种皮色素沉着的功能差异。 (2)喜树碱的产生：从蛇根草的毛状根中对喜树碱生物合成途径的候选基因进行了分析。分析RNAi毛状根的代谢组变化并预测基因功能。对几种植物和人类的喜树碱靶蛋白拓扑异构酶 I 的氨基酸序列进行了比较。在产生喜树碱的植物中发现了导致对喜树碱自身抗性的氨基酸取代。 (3)喹里西啶生物碱（羽扇豆生物碱）：使用含有生物碱的“苦味型”和不含生物碱的“甜味型”羽扇豆，对“苦味特异性”基因进行了分析。使用在大肠杆菌中表达的重组蛋白，进行脱羧酶的酶学表征。比较含喹啉西啶生物碱的物种中该酶的氨基酸序列，发现了几种常见的取代。

项目成果

Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor

• DOI: 10.1111/j.1365-313x.2005.02371.x
• 发表时间: 2005-04-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Tohge, T;Nishiyama, Y;Saito, K
• 通讯作者: Saito, K

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

• 发表时间: 2005
• 作者: Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
• 通讯作者: 星野 幹雄

Enhanced radical scavenging activity of genetically modified Arabidopsis seeds

• DOI: 10.1007/s10529-005-0683-7
• 发表时间: 2005-03-01
• 期刊: BIOTECHNOLOGY LETTERS
• 影响因子: 2.7
• 作者: Tohge, T;Matsui, K;Saito, K
• 通讯作者: Saito, K

Medicinal Plant Biotechnology: From Basic Research to Industrial Applications

• 发表时间: 2006
• 作者: O. Kayser;W. Quax

Covergent evolution in the BAHD family of acyltransferases; identification and characterization of anthocyanin acyltransferases from Arabidopsis thaliana

BAHD 酰基转移酶家族的 Covergent 进化；

• 发表时间: 2007
• 期刊: Plant J □ (OnlineEarly Articles)
• 作者: Koichi Kobayashi;Tatsuru Masuda;Ken-ichiro Takamiya;Hiroyuki Ohta;朽名 夏磨;佐野 俊夫;Jie Luo et al.
• 通讯作者: Jie Luo et al.