The mechanism of bio-motor by using single nano-manipulation
The mechanism of bio-motor by using single nano-manipulation
批准号:
10558108
负责人:
ISHIJIMA Akihiko
金额:
$4.1万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B).
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 2000
中文摘要
在虾纲细胞中,内质网流沿着肌动蛋白索的速度为30-100 μm/s。这种滑动速度比骨骼肌无负荷缩短速度(10 μm/s)快约5倍。然而,关于内质流这种高速滑动的机制还没有完全弄清楚。我们希望在单分子水平上对该轮藻肌球蛋白进行动态测量,以阐明其产生高滑动速度的分子机制。
英文摘要
In Characean cells, the endoplasm streams along the actin cables are at 30-100 μm/s. This sliding velocity is about 5 times faster than no load shortening velocity of skeletal muscle(10 μm/s). However, the mechanism about this high sliding speed of the endoplasm streams haven' t been understood yet. We want to perform dynamic measurement on single molecular level of this Chara myosin to elucidate the molecular mechanism that can produce high sliding speed.
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Akihiko Ishijima, Hiroaki Kojima, Hiroto Tanaka and Toshio Yanagida: "Simultaneous Mesaurement of Individual ATPase and Mechanical Reactions by a Single Myosin Molecule at Work"Optical Review. vol.6, no.1. 16-23 (1999)
Akihiko Ishijima、Hiroaki Kojima、Hiroto Tanaka 和 Toshio Yanagida:“同时测量单个肌球蛋白分子在工作中的单个 ATP 酶和机械反应”光学评论。
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通讯作者:
A.Ishijima, H.Kojima and H.Tanaka: "Simultaneous Observation of Individual ATPase and Mechanical Events by a Single Myosin Molecule during Interaction with Actin."Bio-physics. 39. 76-80 (1999)
A.Ishijima、H.Kojima 和 H.Tanaka:“在与肌动蛋白相互作用期间单个肌球蛋白分子同时观察单个 ATP 酶和机械事件。”生物物理学。
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A.Ishijima, H.Kojima and T.Yanagida: "Single molecule imaging and nano-manipulation"Pathology and Clinic. 16. 1005-1011 (1998)
A.Ishijima、H.Kojima 和 T.Yanagida:“单分子成像和纳米操作”病理学和临床。
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石島秋彦 他: "生体分子1個のイメージング,ナノ操作" 病理と臨床. Vol.16. 1005-1011 (1998)
Akihiko Ishijima 等人:“单个生物分子的成像和纳米操作”病理学和临床研究,第 16 卷,1005-1011 (1998)。
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A.Ishijima, H.Kojima, M.Tokunaga and T.Yanagida: "Single Myosin Imaging of Bio function"PNE. 43. 1365-1371 (1998)
A.Ishijima、H.Kojima、M.Tokunaga 和 T.Yanagida:“生物功能的单肌球蛋白成像”PNE。
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共 17 条
Comprehensive research of signal transduction of cell
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批准号:16H04777
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Signal transduction in cell is only one way?
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批准号:26610127
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财政年份:2014
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Developed the flat top laser illumination system by using two axicon lens
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批准号:24657098
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财政年份:2012
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负责人:ISHIJIMA Akihiko
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Analysis of the relationship between Chemotaxis system and rotational motion of motors of the bacteria by the multi-motor measurements
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批准号:22657038
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.23万
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财政年份:2010
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Energy and information transduction of bacteria flagellar motor of chimeric cell
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财政年份:2008
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依托单位:
Clarification of the energy and signal transduction of bacterial flagellar motor using hybrid motor
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批准号:17076005
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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财政年份:2005
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负责人:ISHIJIMA Akihiko
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Clarification of energy transduction of bacterial flagellar motor using hybrid bacteria
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负责人:ISHIJIMA Akihiko
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海外基金