Technical Developments in the Biological Applications of Scanning Force Microscopy (SFM). Development of an SFM-Based Nano-Manipulation Instrument

该项目涉及开发和增强扫描力显微镜（SFM）的生物标本成像。SFM的新技术能力将被探索，以改善方法来研究多蛋白质-DNA复合物的SFM在空气中，包括一种新的方法来标记这些复合物的SFM研究和一种方法来确定蛋白质诱导的DNA弯曲。此外，还将开发一种基于可持续森林管理的纳米操纵器仪器，并探索生产更锋利探针的方法，以提高可持续森林管理的空间分辨率。这些增强的组合应大大扩展可持续森林管理的适用性，以生物系统。

This project involves the development and enhancement of scanning force microscopy (SFM) for imaging of biological specimens. New technical capabilities for SFM will be explored to improve methods to investigate multi-protein-DNA complexes by SFM in air, including a novel method to label these complexes for SFM studies and a method to determine protein-induced DNA bending. In addition, an SFM-based nanomanipulator instrument will be developed and methods for the production of sharper probes will be explored to improve the spatial resolution of the SFM. The combination of these enhancements should greatly expand the applicability of SFM to biological systems.