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Clarification of the energy and signal transduction of bacterial flagellar motor using hybrid motor

Clarification of the energy and signal transduction of bacterial flagellar motor using hybrid motor
使用混合电机阐明细菌鞭毛电机的能量和信号转导
批准号:
17076005
负责人:
ISHIJIMA Akihiko
金额:
$52.8万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2005
资助国家:
日本
项目状态:
已结题
起止时间:
2005 至 2009

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中文摘要
翻译
细菌鞭毛马达是一种旋转分子机器,它旋转螺旋细丝,推动许多种类的游泳细菌。转子是细胞质膜中直径达45 nm的一组环3;定子包含约10个扭矩产生单元,其锚定在转子周边的细胞壁4,5。马达的自由能源是跨细胞质膜的离子的向内定向的电化学梯度,分别为H1驱动和Na 1驱动马达的质子动力或钠动力。在这里,我们展示了一个步进运动的钠驱动的嵌合体鞭毛电机在大肠杆菌6在低钠动力和控制表达少量的扭矩产生单位。我们观察到每转26步,这与FliG蛋白环的周期性一致,这是转子上扭矩产生的建议位点7,8。向后的步骤,尽管没有鞭毛开关蛋白CheY表明每一步的自由能的变化很小,类似于一个单一的离子过境。
英文摘要
The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel many species of swimming bacteria. The rotor is a set of rings up to 45nm in diameter in the cytoplasmic membrane3 ; the stator contains about ten torque generating units anchored to the cell wall at the perimeter of the rotor4, 5. The free-energy source for the motor is an inward directed electrochemical gradient of ions across the cytoplasmic membrane, the protonmotive force or sodium-motive force for H1-driven and Na1-driven motors, respectively. Here we demonstrate a stepping motion of a Na1-driven chimaeric flagellar motor in Escherichia coli6 at low sodium-motive force and with controlled expression of a small number of torque-generating units. We observe 26 steps per revolution, which is consistent with the periodicity of the ring of FliG protein, the proposed site of torque generation on the rotor7, 8. Backwards steps despite the absence of the flagellar switching protein CheY indicate a small change in free energy per step, similar to that of a single ion transit.
期刊论文(144)
专著(0)
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会议论文
Thermodynamic control of bacterial flagellar motors
细菌鞭毛马达的热力学控制
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Masayoshi Nishiyama, Yoshiyuki Sowa, Shigeichi Kumazaki, Yoshifumi Kimura, Michio Homma, Akihiko Ishijima, Masahide Terazima]
通讯作者: Masahide Terazima
細菌環境応答シグナル伝達の分子機構
细菌环境反应信号的分子机制
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [福岡創, 石島秋彦]
通讯作者: 石島秋彦
Role of the HAMP domain of the bacterial chemoreceptor in signaling and subcellular localization
细菌化学感受器的 HAMP 结构域在信号传导和亚细胞定位中的作用
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Ohta, N., Irieda, H., Banno, S., Homma, M., Kawagishi, I]
通讯作者: I
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Hiremath, G., Ebisawa, T., Hya kutake, A., Banno, S., Nishiyama, S., Kawagishi, I]
通讯作者: I
127
    Comprehensive research of signal transduction of cell
    • 批准号:
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    • 项目类别:
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    • 资助金额:
      $11.32万
    • 财政年份:
      2016
    • 负责人:
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    • 依托单位:
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    • 批准号:
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    • 项目类别:
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    • 资助金额:
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    • 财政年份:
      2016
    • 负责人:
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    • 依托单位:
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    Developed the flat top laser illumination system by using two axicon lens
    • 批准号:
      24657098
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.58万
    • 财政年份:
      2012
    • 负责人:
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    • 依托单位:
    海外基金