Analysis of the relationship between Chemotaxis system and rotational motion of motors of the bacteria by the multi-motor measurements

通过多电机测量分析细菌趋化系统与电机旋转运动的关系

• 批准号: 22657038
• 负责人: ISHIJIMA Akihiko
• 金额: $ 2.23万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657038/
• 关键词: 1分計測; 生物物理; ナノバイオ; ナノマシン; 分子モーター; バクテリア; 回転計測; モータータンパク; 温度; トルク

The bacteria flagellar motor is a motor of which diameter is only 40nm, and rotate about 1000 rotations per second using flow of ions. E. Coli has about 5 filaments and changes these directions by the information from chemo-receptors. Although, the bacteria has works with highly sophisticated signal transduction, energy conversion, and cooperation system, we have a few information about them. In this research, by fusing nano-measurements system and imaging system, the relationship between each motors, the response of the motors by the chemo-signals, the response of the motor by the external stimuli, the correlation between the local stimulation by the caged-compounds and the response of the cell and the correlation between the regulation of the gene expression an rotational motion of the motor.Using these techniques, we found that there were correlations of the switching of each motors, and this timing has some delay. This delay was depended on the position of the each motors, that suggested that the information from the cell pole propagated as wave. Using caged-compounds, the rotational directions of the motors, CW-bias were changed.

细菌鞭毛马达是直径仅为40nm的马达，利用离子流每秒旋转约1000转。大肠杆菌有大约 5 根细丝，并根据化学受体的信息改变这些方向。尽管这种细菌具有高度复杂的信号转导、能量转换和协作系统，但我们对它们的了解还很少。在本研究中，通过融合纳米测量系统和成像系统，研究了每个电机之间的关系、电机对化学信号的响应、电机对外部刺激的响应、笼状化合物的局部刺激与细胞的响应之间的相关性以及基因表达的调节与电机的旋转运动之间的相关性。利用这些技术，我们发现每个电机的切换都存在相关性。 电机，并且这个时间有一些延迟。这种延迟取决于每个电机的位置，这表明来自电池极的信息以波的形式传播。使用笼式化合物，改变了电机的旋转方向、CW 偏压。

项目成果

Construction of the optical system detecting the orientation of molecule from the polarization of fluorescence

根据荧光偏振检测分子方向的光学系统的构建

• 发表时间: 2010
• 作者: Yuichi Inoue;Hajime Fukuoka;Akihiko Ishijima;今野卓;Takahiro Shimizu
• 通讯作者: Takahiro Shimizu

Coordinated Reversal of Flagellar Motors on a Single Escherichia coli Cell

• DOI: 10.1016/j.bpj.2011.03.030
• 发表时间: 2011-05-04
• 期刊: BIOPHYSICAL JOURNAL
• 影响因子: 3.4
• 作者: Terasawa, Shun;Fukuoka, Hajime;Ishijima, Akihiko
• 通讯作者: Ishijima, Akihiko

Adhesion effects of a guanidinium ion appended dendritic "molecular glue" on the ATP-driven sliding motion of actomyosin.

• DOI: 10.1002/anie.200906139
• 发表时间: 2010-04
• 期刊: Angewandte Chemie
• 作者: Kou Okuro;K. Kinbara;Kuniaki Takeda;Y. Inoue;A. Ishijima;T. Aida

Two methods of temperature control for single-molecule measurements

• DOI: 10.1007/s00249-010-0667-y
• 发表时间: 2011-05-01
• 期刊: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
• 影响因子: 2
• 作者: Baker, Matthew A. B.;Inoue, Yuichi;Berry, Richard M.
• 通讯作者: Berry, Richard M.

Measurement of rotational behavior of flagellar motors in E.coli induced by caged serine

笼状丝氨酸诱导的大肠杆菌鞭毛马达旋转行为的测量

• 发表时间: 2010
• 作者: Yuichi Inoue;Hajime Fukuoka;Akihiko Ishijima;今野卓;Takahiro Shimizu;井上雅文;Hiroshi Matsutaka;井上雅文;Takashi Sagawa;井上雅文;Yu Kikushi
• 通讯作者: Yu Kikushi