SR-X-ray fluorescence analysis of arsenic poisoning biopsy samples

砷中毒活检样本的 SR-X 射线荧光分析

• 批准号: 11440219
• 负责人: NAKAI Izumi
• 金额: $ 7.04万
• 依托单位: Faculty of Science, Science Univ.of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11440219/
• 关键词: arsenic poisoning; X-ray fluorescence analysis; two dimensional analysis; X-ray micro beam; XAFS; synchrotron radiation; chemical state analysis; biopsy samples; 放射光蛍光X線分析; 毛髪断面; 有機ヒ素; 無機ヒ素; As K-XANES

Synchrotron radiation induced X-ray fluorescence (SR-XRF) analysis was applied to reveal arsenic level in human hair and skin. The samples are obtained from patients of acute and chronic arsenic poisonings. The former specimens were collected from victim of arsenic curry murder occurred at Wakayama city and the latter were from China, where serious arsenic environmental problem exists and chronic poisoning is generated in a few hundred thousands person scale. The distribution of arsenic along the length of a hair was measured by one-dimensional SR-XRF analysis and that in the vertical section was measured by two-dimensional analysis utilizing X-ray microbeam in 5 μm size. The skin samples obtained by biopsy was also examined. Chemical state of arsenic is strongly related with toxicity and therefore it is another important subject of the present study.One dimensional analysis of hairs from victims of arsenic poisoning show that presence of a clear peak of arsenic and high arsenic level … More continued for 1cm length (about two weeks). On the other hand, the results for the samples of chronic poisoning show monotonous distribution of arsenic. Two dimensional analysis of hair section has revealed high accumulation of arsenic at outer rim of the hair whereas zinc and sulfur was uniformly distributed. The distribution of copper was similar to that of arsenic. It is found for the first time that the accumulation of arsenic does happen in the skin from patients of chronic arsenic poisoning. The high arsenic level was observed at a region between corium and epidermis of the skin. XANES analysis of arsenic in the hair of acute poisoning has revealed that arsenic exists in the form of inorganic trivalent state. This result suggests that the arsenic was not metabolized in the acute poisoning. The present study clearly revealed the accumulation mechanism of arsenic in both acute and chronic arsenic poisonings. This study also demonstrated that SR-XRF is a powerful nondestructive technique to reveal distribution and chemical form of trace arsenic in biopsy samples. Less

采用同步辐射诱导X射线荧光(SR-XRF)分析方法，检测人体头发和皮肤中的砷含量。样本来自急性和慢性砷中毒患者。前者标本来自和歌山市发生的砷咖喱谋杀案受害者，后者来自存在严重砷环境问题和数十万人慢性中毒规模的中国。用一维SR-XRF分析方法测量了砷沿头发长度的分布，利用5μm的X射线微束进行了二维分析。对活检获得的皮肤标本也进行了检查。砷的化学状态与毒性密切相关，因此它是本研究的另一个重要主题。对砷中毒患者头发的一维分析表明，存在明显的砷峰和高砷水平的…更多的持续了1厘米长(大约两周)。另一方面，慢性中毒样品的结果显示砷的分布单一。头发切片的二维分析表明，头发外缘砷的积累很高，而锌和硫的分布是均匀的。铜的分布与砷的分布相似。首次发现慢性砷中毒患者皮肤中确实存在砷的蓄积。在皮肤真皮和表皮之间的区域观察到高砷水平。XANES分析表明，急性中毒患者头发中的砷以无机三价形式存在。这一结果表明，砷在急性中毒过程中没有代谢。本研究清楚地揭示了砷在急性和慢性砷中毒中的蓄积机制。这项研究还表明，SR-XRF是一种强大的无损技术，可以揭示活检标本中痕量砷的分布和化学形态。较少

项目成果

Y.Terada et al.: "Development and application of multi-purpose X-ray Fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom.. 28. 461-463 (1999)

Y.Terada 等人：“使用同步加速器和常规 X 射线源的多用途 X 射线荧光分析仪的开发和应用”X 射线光谱.. 28. 461-463 (1999)

Y.Terada: "Development and application of multi-purpose X-ray fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom.. 28. 461-463 (1999)

Y.Terada：“使用同步加速器和常规X射线源的多用途X射线荧光分析仪的开发和应用”X射线光谱.. 28. 461-463 (1999)

Y.Terada, et.al.: "Development and application of multi-purpose X-ray Fluorescence analyzer using synchrotron and conventional X-ray sources"X-ray Spectrom. 28. 461-463 (1999)

Y.Terada 等人：“使用同步加速器和常规 X 射线源的多用途 X 射线荧光分析仪的开发和应用”X 射线光谱。