Cryo ptychography combined with x-ray fluorescence analysis of metals in cells

细胞中金属的冷冻层析术与 X 射线荧光分析相结合

基本信息

  • 批准号:
    9521374
  • 负责人:
  • 金额:
    $ 3.29万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-09-01 至 2018-03-31
  • 项目状态:
    已结题

项目摘要

Summary X-ray fluorescence microscopes (XFM) offer the highest sensitivity for studies of the role of trace metals in cells, and they provide essential information for understanding the ultrastructural targeting of nanoparticles used for potential cancer therapies. With NIH support, we have developed approaches to rapidly freeze cells for the best preservation of trace metal content as well as cellular ultrastructure, and have shown that cryo x- ray fluorescence microscopy can be used to effectively mitigate radiation damage limitations in the Bionanoprobe, an instrument operated at the Advanced Photon Source (APS) at Argonne and open to researchers based on peer-reviewed, no-cost beamtime proposals. In order to complement XFM's ability to quantitatively image trace element distributions, we have developed high throughput x-ray ptychography (a scanned coherent beam imaging method) to go beyond lens limits and simultaneously obtain 18 nm resolution images of frozen hydrated eukaryotic cells, complementing XFM by providing a high resolution view of cellular ultrastructure. We propose here to develop and validate cryo confocal light microscopy of Bionanoprobe- mounted samples to complement XFM with the capability to image selectively labeled proteins, and to move ptychography from 2D to 3D imaging. To validate these approaches and work from the beginning on a crucial biomedical research project, we will do this in the context of ongoing research in the use of DNA-conjugated nanoparticles containing titanium and/or gadolinium that are meant to target mitochondria for the treatment and imaging of prostrate, breast, and other cancers. In this way, we will develop the methods needed to fully realize the investment NIH has already made in the Bionanoprobe, and build upon Argonne's investment in increasing its available access time at a new experimental station at the APS.
总结

项目成果

期刊论文数量(0)
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Chris Johnson Jacobsen其他文献

Chris Johnson Jacobsen的其他文献

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{{ truncateString('Chris Johnson Jacobsen', 18)}}的其他基金

TR&D Project 2: Tissue and cellular elemental distribution, and image correlation
TR
  • 批准号:
    10494059
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
TR&D Project 2: Tissue and cellular elemental distribution, and image correlation
TR
  • 批准号:
    10197970
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
Resource for Quantitative Elemental Mapping for the Life Sciences
生命科学定量元素图谱资源
  • 批准号:
    10494054
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
Resource for Quantitative Elemental Mapping for the Life Sciences
生命科学定量元素图谱资源
  • 批准号:
    10197965
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
Resource for Quantitative Elemental Mapping for the Life Sciences
生命科学定量元素图谱资源
  • 批准号:
    10652601
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
TR&D Project 2: Tissue and cellular elemental distribution, and image correlation
TR
  • 批准号:
    10652607
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
Supplement for Resource for Quantitative Elemental Mapping for the Life Sciences
生命科学定量元素图资源补充
  • 批准号:
    10586510
  • 财政年份:
    2020
  • 资助金额:
    $ 3.29万
  • 项目类别:
Cryo ptychography combined with x-ray fluorescence analysis of metals in cells
细胞中金属的冷冻层析术与 X 射线荧光分析相结合
  • 批准号:
    9899251
  • 财政年份:
    2013
  • 资助金额:
    $ 3.29万
  • 项目类别:
Cryo ptychography combined with x-ray fluorescence analysis of metals in cells
细胞中金属的冷冻层析术与 X 射线荧光分析相结合
  • 批准号:
    8620670
  • 财政年份:
    2013
  • 资助金额:
    $ 3.29万
  • 项目类别:
Cryo ptychography combined with x-ray fluorescence analysis of metals in cells
细胞中金属的冷冻层析术与 X 射线荧光分析相结合
  • 批准号:
    8800559
  • 财政年份:
    2013
  • 资助金额:
    $ 3.29万
  • 项目类别:

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