Molecular genetic studies on kai gene : mechanism of circadian oscillator

kai基因的分子遗传学研究:昼夜节律振荡器的机制

基本信息

  • 批准号:
    11440234
  • 负责人:
  • 金额:
    $ 2.82万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

Since we have started the current project for molecular basis of cyanobacterial circadian oscillator in 1999, the progress of research has been very fruitful and we obtained following results. Parts of these results have been published in Proc. NAS (2000), Cell (2000), Science (2000) and highly appreciated by colleagues.1) ATP/GTP binding of KaiC and circadian rhythmsKaiC protein carries two ATP/GTP binding motifs. We confirmed that KaiC bound to ATP and introduction of mutations to the motifs completely nullified the ATP binding and the circadian rhythms, suggesting this activity is crucial for the oscillation.2) Circadian rhythms in Kai protein level and KaiC phosphorylationProtein level of three Kai proteins showed circadian rhythms with phases delayed by 8 h from mRNA rhythms. KaiC protein were phosphorylated and the level of phosphorylation also showed circadian rhythms.3) SasA, a histidin kinase that interact with RaiC.A sensory histidine kinase that bound with KaiC was cloned. Disruption of SasA lowered KaiC expression and amplitude of the rhythm. These results indicate that SasA function as amplifier of the circadian oscillator by promoting KaiC expression.4) KaiA enhance phosphorylation of KaiCKaiA protein promotes phosphorylation of KaiC both in vivo and in vitro. This results suggest the mechanism of kaiBC gene activation by RaiC.5) A phytochrome-like protein CikA was cloned with collaboration with Golden's labo (Texas A&M). Disruption of CikA lowered phase shift by 5 h dark pulse and suggest that CikA communicate light signal to the oscillator to reset phase.
目前的蓝藻昼夜振荡器分子基础项目自1999年启动以来,取得了丰硕的研究成果,取得了以下成果:部分研究结果已发表在《美国国家科学院院刊》(2000)、《细胞》(2000)、《科学》(2000)上,并得到了同行的高度评价。1) KaiC与昼夜节律的ATP/GTP结合skaic蛋白携带两个ATP/GTP结合基序。我们证实了KaiC与ATP结合,并且在基序中引入突变完全取消了ATP结合和昼夜节律,这表明这种活性对振荡至关重要。2) Kai蛋白水平和KaiC磷酸化水平的昼夜节律表明,3种Kai蛋白的昼夜节律与mRNA节律相延迟8 h。KaiC蛋白被磷酸化,磷酸化水平也有昼夜节律。3) SasA,与RaiC相互作用的组氨酸激酶。克隆了一个与KaiC结合的感觉组氨酸激酶。SasA的破坏降低了KaiC的表达和节律的幅度。这些结果表明,SasA通过促进KaiC的表达,起到了昼夜节律振荡器的放大作用。4) KaiA增强KaiCKaiA蛋白磷酸化,在体内和体外均促进KaiC磷酸化。这些结果提示了RaiC激活kaiBC基因的机制。5)与Golden实验室(Texas A&M)合作克隆光敏色素样蛋白CikA。CikA的中断降低了5小时的暗脉冲相移,表明CikA将光信号传递给振荡器以复位相位。

项目成果

期刊论文数量(37)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nishiwaki T.: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria"Proc. Natl. Acad. Sci.. 97. 495-499 (2000)
Nishiwaki T.:“时钟蛋白 KaiC 的核苷酸结合和自磷酸化作为蓝细菌的昼夜节律过程”Proc。
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  • 影响因子:
    0
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  • 通讯作者:
Kondo, T.: "The circadian clocks of plants and cyanobacteria"Trends Plant Sci.. 4. 171-176 (1999)
Kondo, T.:“植物和蓝细菌的生物钟”Trends Plant Sci.. 4. 171-176 (1999)
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    0
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Iwasaki H: "AKaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria"Cell. 101. 223-233 (2000)
Iwasaki H:“AKaiC 相互作用的感觉组氨酸激酶,SasA,是维持蓝藻中强大的昼夜节律振荡所必需的”细胞。
  • DOI:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nishiwaki, T., Iwasaki, H., Ishiura, M. and T. Kondo: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria."Proc. Natl. Acad. Sci.. 97. 495-499 (2000)
Nishiwaki, T.、Iwasaki, H.、Ishiura, M. 和 T. Kondo:“时钟蛋白 KaiC 的核苷酸结合和自磷酸化作为蓝细菌的昼夜节律计时过程。”Proc。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nishiwaki T: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing prcess of cyanobacteria."Proc. Natl. Acad. Sci.. 97. 495-499 (2000)
Nishiwaki T:“时钟蛋白 KaiC 的核苷酸结合和自磷酸化作为蓝细菌的昼夜节律计时过程。”Proc。
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  • 影响因子:
    0
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KONDO Takao其他文献

KONDO Takao的其他文献

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{{ truncateString('KONDO Takao', 18)}}的其他基金

Circadian pacemaker of cyanobacteria by clock protein KaiC
通过时钟蛋白 KaiC 实现蓝藻的昼夜节律起搏器
  • 批准号:
    24000016
  • 财政年份:
    2012
  • 资助金额:
    $ 2.82万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
Temporal integration of cellular system by circadian clock in cyanobacteria
蓝藻生物钟对细胞系统的时间整合
  • 批准号:
    15GS0308
  • 财政年份:
    2003
  • 资助金额:
    $ 2.82万
  • 项目类别:
    Grant-in-Aid for Creative Scientific Research
Real-time monitoring of gene expression by bioluminescence
通过生物发光实时监测基因表达
  • 批准号:
    11558089
  • 财政年份:
    1999
  • 资助金额:
    $ 2.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular basis of circadian rhythms : function of clock genes
昼夜节律的分子基础:时钟基因的功能
  • 批准号:
    11694199
  • 财政年份:
    1999
  • 资助金额:
    $ 2.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Screening for genes that respond to environmental stimulus by a real-time monitoring of gene expression by bioluminescence
通过生物发光实时监测基因表达来筛选对环境刺激有反应的基因
  • 批准号:
    07554045
  • 财政年份:
    1995
  • 资助金额:
    $ 2.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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