Preparation of chicken myoblast cell line and regulation of gene expression related to the proliferation and differentiation of chicken myoblasts-Base line of control of meat production-

鸡成肌细胞系的制备及鸡成肌细胞增殖分化相关基因表达调控-产肉控制基础系-

• 批准号: 11460125
• 负责人: AKIBA Yukio
• 金额: $ 7.04万
• 依托单位: TOUHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460125/
• 关键词: Skeletal muscle; myoblast; Differentiation; Cloning; MyoD; Myogenin; Chicken; Myo D; myogenin

The improvement of meat production may be requested to meet the expanding demands for foods all over the world. In the present study, preparation of chicken myoblast cell line and regulation of gene expression related to the proliferation and differentiation of chicken myoblasts was conducted to construct the base line for promoting the efficient meat production system.1. Preparation of chicken myoblast cell linea. Chicken myoblasts could be cloned by the colony formation procedure, while the proliferation could not be maintained for several generations.b. b. SV40 Large T was transfected to chicken primary myoblasts for potentiating the proliferation activity. The expression of SV40 Large T was transiently appeared in the cell clone.c. Promoter sequences of specific proteins in chicken muscle cells were subjected for cloning and ligated to plasmid (pd2EGFP-1) which expressed GFP, and then transfected to the chicken primary myoblasts. The analysis is currently continued and the preparat … More ion of chicken muscle cell line is scheduled with aid of this procedure.2. Screening of factors regulating proliferation and differentiation in chicken myoblastsa. In the chicken myoblast culture, MyoD and Myogenin mRNA expression were peaked at 12 and 2h hr, respectively and they were followed by gradual decrease.b. Myosin, CPK activity and MyD mRNA expression increased by addition of prostaglandin E2 (PGE2), suggesting that PGE2 is a factor stimulating the differentiation of muscle cells.c. Proliferation in the myocyte growth stage and fusion of myoblasts in the terminal differentiation was stimulated by addition of beta-hydroxybutyrate (BTB), a ketone body, in the myocyte culture and these effects were modified by the amount of energy source present in the medium.d. Residual yolk present in the abdomen of post-hatched chicks appears to contain a plenty of proteins rich in metabolic function. Addition of the proteins extracted from the residual yolk was no influence on the muscle cell differentiation at the low concentration (1-100 ug) but induced the cell death at the high concentration (1 or 3 mg). Less

为了满足全世界日益增长的食品需求，可能需要改进肉类生产。本研究通过鸡成肌细胞系的制备及成肌细胞增殖分化相关基因的表达调控，构建促进高效产肉体系的基础.鸡成肌细胞系的制备。鸡成肌细胞可通过殖民地形成法克隆，但不能维持数代增殖。B.将SV 40 Large T转染鸡原代成肌细胞以增强其增殖活性。SV 40 Large T在细胞克隆中瞬时表达。将鸡肌细胞特异性蛋白的启动子序列克隆到表达GFP的质粒（pd 2 EGFP-1）中，转染鸡原代成肌细胞。目前正在继续进行分析， ...更多信息 鸡肌肉细胞系的离子化是借助于该方法进行的。鸡成肌细胞增殖与分化调控因子的筛选。在鸡成肌细胞培养中，MyoD和Myogenin mRNA的表达分别在12和2 h达到高峰，随后逐渐下降。加入前列腺素E_2（PGE_2）后，肌球蛋白、CPK活性和MyD mRNA表达均增加，提示PGE_2是促进肌细胞分化的因子。通过在肌细胞培养物中加入β-羟基丁酸酯（BTB）（一种酮体）刺激肌细胞生长阶段的增殖和终末分化中成肌细胞的融合，并且这些作用通过培养基中存在的能量源的量来改变。出壳后雏鸡腹部残留的卵黄中含有丰富的具有代谢功能的蛋白质。低浓度（1-100 μ g）的卵黄蛋白对肌细胞分化无影响，高浓度（1 ~ 3 mg）的卵黄蛋白则诱导细胞死亡。少

项目成果

Toshihiro Takahashi, Kan Sato, Yukio Akiba: "A possible procedure to prepare mono fatty acid-enriched plasma membrane in chicken hepatocytes"Animal Sci.J.. 70. 118-123 (1999)

Toshihiro Takahashi、Kan Sato、Yukio Akiba：“在鸡肝细胞中制备富含单脂肪酸的质膜的可能程序”动物科学杂志 70. 118-123 (1999)

Takahashi,T.,Sato,K. and Akiba,Y.: "A possible procedure to prepare mono fatty acid-enriched plasma membrane in chicken hepatocytes."Anim.Sci.J.. 70. 118-123 (1999)

高桥，T.，佐藤，K.

Takahashi,T.,Sato,K.and Akiba,Y.: "A possible procedure to prepare mono fatty acid-enriched plasma membrane in chicken hepatocytes."Anim.Sci.J.. 70. 118-123 (1999)

Takahashi,T.、Sato,K. 和 Akiba,Y.：“在鸡肝细胞中制备富含单脂肪酸的质膜的可能程序。”Anim.Sci.J.. 70. 118-123 (1999)

Akiba,Y.: "Potential of nutrition in the control of growth and meat quality in broilers."J.Poult.Sci.. 38. 67-80 (2000)

Akiba,Y.：“营养在控制肉鸡生长和肉质方面的潜力。”J.Poult.Sci.. 38. 67-80 (2000)

Nishida,H,Sato,K,Akiba,Y. et al.: "Influence of prostaglandin E2 on chick myoblasts differentiation during the myogenesis."Animal Sci.& Technol.. 68. 381-384 (1997)

西田，H，佐藤，K，秋叶，Y。