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Development of anti-obesity vaccine for domestic animals by regulation of gene expression during adipogensis. -Low fat meat production-

通过调节脂肪形成过程中的基因表达来开发家畜抗肥胖疫苗。

基本信息

批准号：
13556043
负责人：
AKIBA Yukio
金额：
$ 7.68万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

To develop an anti-obesity vaccine for domestic animals and a safety low fat meat production, we investigated 1)the regulatory mechanism of adipocyte differentiation on animal preadipocyte, 2)screening of functional nutrient for the inhibition of adipocyte hyperplasa, and 3)the effect of intraperitoneal administration with inhibitor of adipocyte proliferation on adipose tissue weight.1)The regulatory mechanism of chicken adipocyte differentiationTo induce the chicken pre-adipocyte differentiation, we firstly clarified that the addition of an exogenous fatty acid mixed with hormones (Ins, DEX, IMX), which induce the differentiation of mammalian adipoocyte, was essential for chicken adipogensis. In addition, it is determine that PPARγ is master regulator of chicken adipocyte differentiation during adipogensis.2) Screening of functional nutrient for the inhibition of adipocyte hyperplasaThe adipocyte proliferation declined with the addition of the mixture of insulin. 3-isobutyl-1-methylxanthin, dexamethason and oleic acid, and completely inhibition of cell growth occurred at the concentration of 20μg/ml insulin, 0.5mM 3-isobutyl-1-methylxanthin, 500nM dexamethason and 300μM oleic acid in the media.3)The effect of intraperitoneal administration with inhibitor of adipocyte proliferation on adipose tissue weightIn chickens with single intraperitoneal injection of these hormone and fatty acid mixture made emulsion with triolein, adipose fat pad weight was significantly lower than the control chickens whereas the body weight decreased to a small extent. In addition, the administration of our anti-obesity vaccine regulated in adipocyte hyperplsia.Unfortunately, it was not still known the powerful nutrient, which was easily accepted by consumer, for the regulation of adipocyte hyperplasia, but the new manipulation technique reducing fat deposition, called anti-obesity vaccine, was developed by the control of adipogensis in chickens.

为了研制家畜抗肥胖疫苗和安全的低脂肉制品，我们研究了1)脂肪细胞分化对动物前脂肪细胞的调节机制，2)抑制脂肪细胞增生的功能营养素的筛选，3)腹腔注射脂肪细胞增殖抑制剂对脂肪组织重量的影响。1)鸡脂肪细胞分化的调控机制为了诱导鸡脂肪前细胞分化，我们首先明确了外源脂肪酸与诱导哺乳动物脂肪细胞分化的激素（Ins、DEX、IMX）的混合是鸡脂肪形成的必要条件。此外，我们还确定PPARγ是鸡脂肪形成过程中脂肪细胞分化的主要调控因子。2)抑制脂肪细胞增厚功能营养素的筛选随着胰岛素混合物的加入，脂肪细胞增殖下降。3-异丁基-1-甲基黄嘌呤、地塞米松和油酸，20μg/ml胰岛素、0.5mM 3-异丁基-1-甲基黄嘌呤、500nM地塞米松和300μM油酸对细胞生长有完全抑制作用。3)腹腔注射脂肪细胞增殖抑制剂对脂肪组织重量的影响。单次腹腔注射这些激素和脂肪酸与三油酸混合制成乳化液后，鸡的脂肪垫重量显著低于对照组，而体重略有下降。此外，我们的抗肥胖疫苗的施用调节脂肪细胞增生。不幸的是，当时人们还不知道这种容易被消费者接受的强大营养素对脂肪细胞增生的调节作用，但通过控制鸡的脂肪生成，开发了一种减少脂肪沉积的新操作技术，称为抗肥胖疫苗。