Characterization and regulation of myogenic factors and substrate transporters during embryonic and post-hatch stages in chickens

鸡的特点是血糖水平是人类的两倍，对胰岛素作用有明显的抵抗力。本研究以肉仔鸡为研究对象，探讨了生长早期胰岛素依赖性葡萄糖转运和摄取的特点以及肌肉发生调控因子的特异性.新孵化鸡血浆胰岛素浓度的变化每天测定肉鸡血浆胰岛素浓度，直至第10天，并在第10、14和28天测定血浆胰岛素浓度。出雏后14日龄血浆胰岛素浓度显著低于28日龄.注射胰岛素对生长早期血糖浓度的影响注射胰岛素（10或40μg/kg BW）后60 min血糖浓度随剂量增加而降低。注射胰岛素后1 - 7日龄雏鸡血糖浓度的下降幅度显著大于14日龄和21日龄雏鸡.组织 ...更多信息 在比目鱼肌、趾长伸肌、胸大肌和肝脏中，注射胰岛素可增加2-脱氧-D-[^3H]-葡萄糖的摄取。注射胰岛素的雏鸡肝脏和骨骼肌的葡萄糖摄取量分别比未注射胰岛素的对照雏鸡高6.5倍和2倍.鸡骨骼肌胰岛素依赖性葡萄糖摄取的体外研究0.02μg/ml胰岛素孵育的大鼠骨骼肌葡萄糖摄取比对照组高2倍。与此相反，即使在50倍于大鼠骨骼肌孵育剂量的胰岛素下孵育，也没有观察到鸡骨骼肌中胰岛素相关的葡萄糖摄取。鸡成肌细胞成肌过程中葡萄糖转运蛋白mRNA表达的变化鸡葡萄糖转运蛋白1和3（GLUT 1和3）的mRNA表达在成肌细胞向肌管分化的过程中没有发生变化。少

Chickens were characterized by blood glucose levels that are twice as high as human and have a marked resistance to insulin actions. In present study, characteristics in the insulin dependent glucose-transport and -uptake during early growing phase and the specificities in factors regulating myogenesis were investigated in broiler chickens.1. Changes in plasma insulin concentrations in newly hatched chicksPlasma insulin concentrations were determined every day up to 10 day and days at 10, 14, and 28 in broiler chickens. Plasma insulin concentrations during 14 days post-hatch were significantly lower than 28-days old chickens.2. Effect of insulin injection on plasma glucose concentrations during early growing periodPlasma glucose concentrations at 60 min after insulin injections (10 or 40μg/kg BW) were decreased as the dose increased. The decrease of plasma glucose concentrations after insulin injections was significantly greater at 1 - 7 days old chicks than at 14 and 21 days.3. Tissue … More specificity in insulin responsible glucose uptake in chicksIn Soleus, EDL and Pectoralis muscles and liver, 2-deoxy-D-[^3H]-glucose uptake increased by insulin injection. The glucose uptake in insulin-infused chicks in liver and skeletal muscles was higher by 6.5 and 2 times, respectively, than those in control chicks with no insulin injection.4. Insulin responsible glucose uptake in chicken skeletal muscles in vitroGlucose uptake into rat skeletal muscles incubated with insulin at 0.02μg/ml in vitro was 2 time higher than that of control culture. In contrast, insulin-responsible glucose uptake in chick skeletal muscles was not observed even when muscles were incubated with 50 time higher dose of of insulin used for rat skeletal muscle incubations.5. Change in glucose transporter mRNA expressions during myogenesis in chicken myoblastsThe mRNA expressions of chicken glucose transporter 1 and 3 (GLUT 1 and 3) were not changed during myogenesis, in particular in the differentiation stage from myoblast to myotubes. Less