MOLECULAR AND CELLULAR STUDIES OF MOLECULAR CHANGES OF PROTEINS ASSOCIATED WITH CHROMATIN DURING CYTOKINESIS

细胞分裂过程中与染色质相关的蛋白质分子变化的分子和细胞研究

• 批准号: 11460157
• 负责人: IKEGAMI Susumu
• 金额: $ 9.54万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460157/
• 关键词: starfish; oocyte; nucleolus; myosin; phosphorylation; contractile ring; イトマキヒトデ; トランスグルタミナーゼ; ヒストン; 胞胚; 燐酸化; 細胞分裂; NAAP; GTPase; クロマチン; F-11754a; 胚

Molecular changes of proteins associated with chromatin during cytokinesis have been studied using starfish eggs and embryos. Nucleic acid-associated protein sedignated NAAP has been purified from starfish (Asterina pectinifera) oocytes and an antibody reacting with NAAP has been prepared. Immunofluorscence images of starfish oocytes show that NAAP is primarily located in the nucleolus of the germinal vesicle (nucleus) of the oocyte. The C-terminal region of NAAP has been shown to be associated with chromatin. When starfish oocytes undergo meiotic maturation NAAP is phosphorylated by cdc2 kinase at specific sites and distributed evenly in the cytoplasm at the first meiotic prometa- to anaphases during the first meiotic division. Therefore, it is concluded that phospharylation at specific Ser or Thr residues dissociates NAAP fron chromatin.

利用海星卵和胚胎研究了细胞质分裂过程中染色质相关蛋白的分子变化。从海星卵母细胞中分离纯化了核酸相关蛋白NAAP，并制备了NAAP抗体。海星卵母细胞的免疫荧光图像显示，NAAP主要定位于卵母细胞的生殖囊泡（核）的核仁中。NAAP的C-末端区域已被证明与染色质相关。当海星卵母细胞减数分裂成熟时，NAAP在第一次减数分裂的前中期至后期被cdc 2激酶在特定位点磷酸化，并均匀分布在细胞质中。因此，可以得出结论，在特定的丝氨酸或苏氨酸残基磷酸化解离NAAP从染色质。

项目成果

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Murata-Hori, M. 等人：“Myoshin II 调节轻链作为 AIM-1（一种来自大鼠的 Auror/Ipl1p 相关激酶）的新型底物”J.

Ohta, E. et al.: "Micromonospolide A, a new macrolide from Micromonospora sp"Tetrahedron Lett.. 42. 4179-4181 (2001)

Ohta, E. 等人：“Micromonospolide A，一种来自 Micromonospora sp 的新大环内酯”Tetrahedron Lett.. 42. 4179-4181 (2001)

Komada, S. et al.: "Identification of myosin II kinase from sea urchin eggs as protein kinase CK2"Gene. 275. 141-148 (2001)

Komada, S. 等人：“将海胆卵中的肌球蛋白 II 激酶鉴定为蛋白激酶 CK2”基因。

Hioki, H., Ooi, H., Hamano, M, Mimura, Y., Yoshio, S., Kodama, M., Ohta, S., Yanai, M., Ikegami, S.: "Enantioselective total synthesis and absolute sterestructure of hippospongic acid A"Tetrahedron. 57. 1235-1246 (2001)

Hioki, H.、Ooi, H.、Hamano, M、Mimura, Y.、Yoshio, S.、Kodama, M.、Ohta, S.、Yanai, M.、Ikegami, S.：“对映选择性全合成和绝对

Ohta, E., Kubota, N., Suzuki, M., Ogaawa, T., Ohta, S., Ikegami, S: "Micromonospolide A, a new macrolide from Micromonospora sp."Tetrahedron Lett.. 4179-4181 (2001)

Ohta, E.、Kubota, N.、Suzuki, M.、Ogaawa, T.、Ohta, S.、Ikegami, S：“Micromonospolide A，一种来自 Micromonospora sp. 的新大环内酯”Tetrahedron Lett.. 4179-4181 (2001