Mechanisms of cell division with protein phosphorylation.

蛋白质磷酸化的细胞分裂机制。

• 批准号: 06454138
• 负责人: IKEGAMI Susumu
• 金额: $ 4.67万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454138/
• 关键词: Myosin; Phosphorylation; P1; NAAP; cdc2 kinase; Cyclin B; ヒトデ; 細胞核; 二重鎖RNA; 卵細胞; cDNA; 卵母細胞; ハレナキノール・サルフェート; ジャスピシン; イリジャスピシン; DNAポリメラーゼ; 細胞膜融合; 孵化酵素; ゲラチナーゼA; 蛋白質セリン・キナーゼ

Hosoya and his colleagues discovered that phosphorylated myosin is present in the contractile ring. This is a very importand discovery for the understanding of the mechanism of cell division. They also discovered the enzyme that selectively phosphorylates the light chain of myosin.Shioda and his colleagues discovered a novel protein, P1, which regulates the initiation of DNA replication. P1, which is inactive in its unphosphorylated form, becomes active by phosphorylation. The enzyme responsible for the phosphorylation is found to be a DNA-dependent kinase.Ikegami and his colleagues discovered a novel nucleic-acid associated protein designated as NAAP which was isolated from starfish oocytes. Oligonucleotides derived from the partial amino acid sequences of NAAP were used to clone NAAPcDNA and the entire cDNA sequence was determined. The transcript encoding NAAP was present only in growing oocytes but not in full-grown oocytes, embryos and adult tissues. The NAAP protein localized in the germinal vesicle of the full-grown oocyte and dispersed diffusely after germinal vesicle breakdown at the onset of meiosis. During oocyte maturation, NAAP changed the moleclar form in a cell-cycle dependent manner. It was found that Ser 145 of NAAP,which was unphosphorylated at the germinal vesicle stage, was phosphorylated during the M phase of meiosis.

Hosoya和他的同事发现磷酸化肌球蛋白存在于收缩环中。这对于理解细胞分裂的机制是一个非常重要的发现. Shioda和他的同事们发现了一种新的蛋白质P1，它可以调节DNA复制的起始。P1在其未磷酸化形式下是无活性的，通过磷酸化变得有活性。Ikegami等人从海星卵母细胞中分离到一种新的核酸相关蛋白，命名为NAAP。从NAAP的部分氨基酸序列衍生的寡核苷酸被用来克隆NAAPcDNA，并确定整个cDNA序列。编码NAAP的转录本仅存在于生长的卵母细胞中，而不存在于完全生长的卵母细胞、胚胎和成体组织中。NAAP蛋白定位于成熟卵母细胞的萌发囊泡中，并在成熟卵母细胞减数分裂开始时萌发囊泡破裂后弥散分布。在卵母细胞成熟过程中，NAAP以细胞周期依赖的方式改变分子形态。结果表明，在芽囊泡期未被磷酸化的NAAP的Ser145在减数分裂的M期被磷酸化。

项目成果

Ohta,S.: "Hipposrngic acid A : an unusuel triterpencic acid from a sponge." Tetrahedron letters. 37. 7765-7766 (1996)

Ohta,S.：“Hipposrngic Acid A：一种来自海绵的独特三萜酸。”

Ikegami,S.et al.: "Selective inhibition of exoplasmic membrane fusion in Echinoderm gametes with jaspisin,a novel antihatching substance isdated from a marine sponge." J.Biological Chemistry. 269. 23262-23267 (1994)

Ikegami,S.et al.：“用 Jaspisin 选择性抑制棘皮动物配子的外质膜融合，jaspisin 是一种来自海绵的新型抗孵化物质。”

Shioda.M.et al.: "Differential inhibition of eukaryotic DNA polymerares by halenaquinal sulfate,a p-hydroquinone sulfate obtained from a marine sponge." FEBS Letters. 350. 249-252 (1994)

Shioda.M.等人：“硫酸海藻醌（一种从海绵中获得的对苯二酚硫酸盐）对真核 DNA 聚合体的差异抑制。”

Uno, M., Ohta, S., Ohta, E., and Ikegami, S.: "Callyspongins A and B : Novel polyacetylene sulfates from the marine sponge, Callyspongia truncata, that inhibit fertilization of starfish gametes." J.Natl.Prod.(Lloydia). 59. 1146-1148 (1996)

Uno, M.、Ohta, S.、Ohta, E. 和 Ikegami, S.：“Callyspongins A 和 B：来自海绵 Callyspongia truncata 的新型聚乙炔硫酸盐，可抑制海星配子的受精。”

T. Shimizu et al.: "A covalently crosslinked histone" Nature. 380. 32 (1996)

T. Shimizu 等人：“共价交联的组蛋白”自然。