Molecular, cellular and phylogenetic aspects of selective inhibition of early periods in metazoan ontogenesis by sponge and other substances

海绵和其他物质选择性抑制后生动物个体发生早期的分子、细胞和系统发育方面

• 批准号: 23658102
• 负责人: IKEGAMI Susumu
• 金额: $ 2.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658102/
• 关键词: ヒトデ発生; 発生阻害; Ancorinoside A; Exiguamide; 若狭湾

Following fertilization, the starfish, Asterina pectinifera, embryo develops to the blastula and then undergoes gastrulation to form a gut and create a feeding bipinnaria larva. The larva then takes on a more elaborate form called a brachioraria larva. Several substances obtained from sponges specifically prevent starfish embryonic development at the blastula or the gastrula stages. Screening search for synthetic compounds which mimic the action of these substances was undertaken but such compounds were not detected.The study was then focused on the effect of loss of nuclear transglutaminase, a protein that is phylogenetically unique in the starfish embryo, by microinjecting the morpholino antisense oligonucleotide. The injected embryo developed normally through the embryonic and bipinnaria stages but was unable to form normal brachioraria. This result suggests that the unique protein is involved in the morphogenetic event at the latter period in the starfish ontogeny.

受精后，海星（Asterina pectinifera）胚胎发育成囊胚，然后经历原肠胚形成，形成肠道并产生摄食的双羽藻幼虫。然后幼虫呈现出一种更复杂的形态，称为臂形幼虫。从海绵中获得的几种物质专门阻止海星在囊胚或原肠胚阶段的胚胎发育。 对模拟这些物质作用的合成化合物进行了筛选，但没有检测到此类化合物。然后，该研究的重点是通过显微注射吗啉代反义寡核苷酸，核转谷氨酰胺酶（一种在海星胚胎中系统发育上独特的蛋白质）丢失的影响。注射的胚胎在胚胎期和双羽期正常发育，但无法形成正常的臂形。这一结果表明，该独特的蛋白质参与了海星个体发育后期的形态发生事件。

项目成果

Characterization of O-GlcNAcylation in Starfish (Asterina pectinifera) Development from Fertilization to Bipinnaria Larva

海星 (Asterina pectinifera) 从受精到双翅幼虫发育过程中 O-GlcNAc 酰化的表征

• DOI: 10.1271/bbb.100601
• 发表时间: 2011
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Ogawa;M.;Adachi;T.;Ikegami;S.;Kato;K. H.;Yamamoto;A.;& Kamemura;K
• 通讯作者: K

Bromotheoynic acid, a brominated acetylenic acid from the marine sponge Theonella swinhoei

Bromotheoynic Acid，一种来自海洋海绵 Theonella swinhoei 的溴化乙炔酸

• DOI: 10.1080/14786419.2012.660636
• 发表时间: 2012
• 期刊: Natural Product Research
• 影响因子: 2.2
• 作者: N.Aoki;K.Yamamoto;T. Ogawa;E.Ohta;T.Ikeuchi;K.Kamemura;S.Ikegami;S.Ohta
• 通讯作者: S.Ohta

Petroacetylene, a new polyacetylene from the marine sponge Petrosia solida that inhibits blastulation of starfish embryos.

石油乙炔，一种来自海洋海绵 Petrosia Solida 的新型聚乙炔，可抑制海星胚胎的胚芽发育。

• DOI: 10.1080/14786419.2012.763128
• 发表时间: 2013
• 期刊: Natural Product Research
• 影响因子: 2.2
• 作者: Ohta;S.;Ogawa;T.;Ohta;E.;Ikeuchi;T.;Kamemura;K.;and Ikegami;S.
• 通讯作者: S.

イトマキヒトデ胚発生における核型トランスグルタミナーゼの機能解析

海星胚胎发育过程中核型转谷氨酰胺酶的功能分析

• 作者: 池上 晋;古川亮平;金子洋之
• 通讯作者: 金子洋之

イトマキヒトデ胚における間充織細胞の分化

海星胚胎间充质细胞的分化

• 作者: 池上 晋;清水孝彦;金子洋之
• 通讯作者: 金子洋之