Analysis of HTLV-I infection mechanisms using cell-free virus

1)我们发现HSC70的表达增强了HTLV-I诱导的细胞融合，但不影响无细胞HTLV-I的镀效率，这表明HSC70与HTLV-I结合，而不是其受体。2)当表达HTLV-I的Tax的8C细胞感染无细胞HTLV-I后，这些细胞内形成大量合胞体，培养上清中检测到子代HTLV-I的产生。3)嵌合疱疹病毒（chimeric vesicular stomatitis virus， VSV）携带htlv - 1包膜蛋白的GFP基因，在产生htlv - 1的细胞感染后可有效形成假型病毒，即嵌合疱疹病毒（chimeric vesicular stomatitis virus， VSV）。4)我们发现htlv - 1对紫外光高度敏感，但在37℃下的稳定性较牛白血病病毒（bovine leukemia virus， BLV）差。5)当人滑膜细胞感染HTLV-I或BLV时，在感染后不久检测到两种病毒。感染BLV而未感染htlv - 1的细胞在组织培养中生长1年以上。6)一个包含gp21的寡肽在病毒吸附后抑制HTLV-I感染。7) htlv - 1感染者经病毒吸附步骤后，血清对htlv - 1感染有抑制作用。8)经糖基化抑制剂处理的细胞产生的HTLV-I，特别是糖基化早期抑制剂的感染性降低。唾液酸酶则增强了其传染性。9)将HTLV-I感染或tax转导的人骨肉瘤细胞注射到裸鼠体内，可引起肿瘤快速形成、脾肿大和中性粒细胞增多。血清中高水平检测到G-CSF或GM-CSF。

1) We showed that the expression of HSC70 enhanced HTLV-I-induced cell fusion but did not affect the efficiency of cell-free HTLV-I plating, suggesting that HSC7O binds to HTLV-I but is not its receptor.2) When 8C cells expressing Tax of HTLV-I were infected with cell-free HTLV-I, numerous syncytia were formed in these cells and production of progeny HTLV-I was detected in culture supernatants.3) Pseudotype virus, namely, chimeric vesicular stomatitis virus (VSV) containing GFP gene that bears envelope proteins of HTLV-I, was formed efficiently when HTLV-I-producing cells were infected with chimeric VSV.4) We found that HTLV-I was highly sensitive to ultraviolet light irradiation and that it was not so stable at 37℃, as compared with bovine leukemia virus (BLV).5) When human synovial cells were infected with HTLV-I or BLV, both viruses were detected shortly after infection. The cells infected with BLV but not HTLV-I grew more than one year in tissue culture.6) One of oligopeptides encompassing gp21 inhibited HTLV-I infection at a step after virus adsorption.7) Sera of HTLV-I-infected subjects inhibited HTLV-I infection in vitro after virus adsorption step.8) HTLV-I produced from cells treated with glycosylation inhibitors, especially inhibitors at early steps of glycosylation showed reduced infectivities. In contrast, sialidase enhanced its infectivity.9) Injection of human osetosarcoma cells infected with HTLV-I or transduced with tax into nude mice induced rapid tumor formation, splenomegaly and neutrophilia. G-CSF or GM-CSF was detected in their sera at high levels.