Analyses of effects of heavy ion irradiation on expression of viral and cellular genes

重离子辐照对病毒和细胞基因表达的影响分析

• 批准号: 17390133
• 负责人: HOSHINO Hiroo
• 金额: $ 8.96万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390133/
• 关键词: Heavy ion; Virus; Gene; Mutation; Susceptibility

We have analyzed the infection mechanism of viruses, such as HIV-1, HBV or HCV, using cells irradiated with heavy ions and inoculated with them. As heavy ions, He, C or Ne was used and as controls, irradiation with UV or X-ray/γ ray, or treatment with various chemical compounds was used. As target cells for irradiation, the human cell lines C8166 and NP-2/CD4/CCR5 (NP2 cells transduced with CD4 and CCR5 genes), HBV-positive Alexander cell line or HCV replicon-positive Huh-7 line was used.The cells were irradiated with heavy ions and then infected with HIV-1. The amount of reverse transcribed viral DNA was increased in the irradiated cells. Irradiation with higher doses did not induce this increase. The amount of HIV-1 adsorbed to the cells did not increase significantly. These results suggest that irradiation of cells with relatively low doses of heavy ions will promote incorporation of HIV-1 virions or reverse transcription as well as transcription of viral RNA.Irradiation of C8166 ce … More lls harboring HTLV-I with heavy ions or X-ray enhanced expression of NFκB, whereas expression of HTLV-I Tax was not affected by the irradiation. In HBV-positive cells, heavy ion irradiation tended to enhance the production of HBV antigens. In HCV replicon-positive Huh-7 cells, heavy ion irradiation did not apparently affect the number of HCV replicon.In cells irradiated with heavy ions, expression of CD133 mRNA or NFκB mRNA was enhanced, whereas mRNA for histon deacetylase (HDAC), Ku80 or poly-ADP ribose polymerase was decreased.Analyses of susceptibilities of irradiated cells to virus infection showed the specific effects exerted by irradiation with heavy ions, but not by irradiation with UV or X-ray. The difference in biological effects between heavy ion irradiation and X-ray irradiation has generally been considered to be present merely in their relative biological effects (RBE). Our study suggested that there is a qualitical difference between irradion with heavy ions and X-ray or UV. Less

我们已经分析了病毒的感染机制，如HIV-1，HBV或HCV，使用重离子辐照的细胞，并接种它们。作为重离子，使用He、C或Ne，并且作为对照，使用UV或X射线/γ射线照射或用各种化合物处理。作为照射的靶细胞，使用人细胞系C8166和NP-2/CD 4/CCR 5（用CD 4和CCR 5基因转导的NP-2细胞）、HBV阳性亚历山大细胞系或HCV复制子阳性Huh-7细胞系。在照射的细胞中，逆转录病毒DNA的量增加。较高剂量的辐照不会引起这种增加。吸附到细胞上的HIV-1的量没有显著增加。这些结果表明，用相对低剂量的重离子辐照细胞将促进HIV-1病毒粒子的掺入或逆转录以及病毒RNA的转录。 ...更多信息 重离子或X射线照射后HTLV-Ⅰ的表达增强NFκB的表达，而HTLV-Ⅰ Tax的表达不受照射的影响。在HBV阳性细胞中，重离子辐照倾向于增强HBV抗原的产生。在HCV复制子阳性的Huh-7细胞中，重离子辐照对HCV复制子的数量无明显影响。重离子辐照后，细胞中CD 133 mRNA和NFκB mRNA的表达增强，而希斯顿脱乙酰酶（HDAC）、Ku 80和聚ADP核糖聚合酶的mRNA表达降低。重离子辐照细胞对病毒感染的敏感性分析表明，重离子辐照具有特异性效应，但不能通过UV或X射线照射。重离子辐照和X射线辐照的生物效应差异一般被认为仅仅存在于它们的相对生物效应（RBE）中。我们的研究表明，重离子辐照与X射线或紫外线辐照有本质的区别。少

项目成果

Formation of Vesicular Stomatitis Virus Pseudotypes Bearing Surface Proteins of Hepatitis B Virus

携带乙型肝炎病毒表面蛋白的水泡性口炎病毒假型的形成

• DOI: 10.1128/jvi.79.19.12566-12574.2005
• 发表时间: 2005
• 期刊: Journal of Virology
• 影响因子: 5.4
• 作者: M. Saha;A. Tanaka;Atsushi Jinno;N. Shimizu;K. Tamura;M. Shinagawa;J. Chiba;H. Hoshino
• 通讯作者: H. Hoshino

The synthetic peptide derived from the NH2-terminal extracellular region of an orphan G protein-coupled receptor, GPR1, preferentially inhibits infection of X4 human immunodeficiency virus type 1

源自孤儿 G 蛋白偶联受体 GPR1 NH2 末端胞外区的合成肽优先抑制 X4 人类免疫缺陷病毒 1 型的感染

• 发表时间: 2005
• 期刊: Journal of Biological Chemistry 280
• 作者: Jinno-Oue;A.;N.Shimizu;Y.Soda;A.Tanaka;T.Otsuki;D.Kurosaki;Y.Suzuki;H.Hoshino
• 通讯作者: H.Hoshino

Construction and in vitro characterization of a chimeric simian and human immunodeficiency virus with the RANTES gene.

具有 RANTES 基因的嵌合猿猴和人类免疫缺陷病毒的构建和体外表征。

• 发表时间: 2006
• 期刊: Microbes Infect. 8
• 作者: Shimizu;Y.;Okoba;M.;Yamazaki;N.;Goto;Y.;Miura;T.;Hayami;M.;Hoshino;H.;Haga T.
• 通讯作者: Haga T.

Tandem repeats of lactoferrin-derived anti-hepatitis C virus peptide enhance antiviral activity in cultured human hepatocytes

• DOI: 10.1111/j.1348-0421.2007.tb03882.x
• 发表时间: 2007-01-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Abe, Ken-ichi;Nozaki, Akito;Kato, Nobuyuki
• 通讯作者: Kato, Nobuyuki

Isolation of the feline alpha1,3-galactosyltransferase gene, expression in transfected human cells and its phylogenetic analysis.

猫α1,3-半乳糖基转移酶基因的分离、在转染的人类细胞中的表达及其系统发育分析。

• 发表时间: 2006
• 期刊: J Exp Zoolog B Mol Dev Evol 306
• 作者: Roy BB;Jinno-Oue A;Shinagawa M;Shimizu A;Tamura K;Shimizu N;Tanaka A;Hishino H.
• 通讯作者: Hishino H.