Chromosome translocation junction genes on lympho-hematopoietic cell proliferation and differentiation

我们报道了染色体18q21上的一个新基因MALT1，该基因参与粘膜相关淋巴组织（MALT）淋巴瘤t(11;18)（q21;q21）。这种易位导致API2在11q21位点和MALT1在18q21位点融合，产生API2-MALT1嵌合产物。我们目前正在尝试生产针对API2和MALT1产物的单克隆抗体，以便我们可以用免疫组织化学方法检查MALT淋巴瘤病例。我们研究了弥漫性大B细胞淋巴瘤（DLBCL），已知它包含几个亚型，尽管在不同的血液病理学家中很难获得亚型分类的共识。我们检测了BCL1、BCL2、BCL6基因重排及免疫染色。我们发现细胞表面标记物可以作为描述临床显著亚型的标记物。事实上，我们发现CD5+ DLBCL组成了一组预后不良的患者，其预后与套细胞淋巴瘤一样差。易位连接基因与t细胞淋巴瘤的关系尚不清楚。基于已有报道t细胞淋巴瘤在6q21位点有频繁的染色体畸变，我们试图分离相关基因。我们在t细胞淋巴母细胞样淋巴瘤细胞系HT-1和成人t细胞白血病/淋巴瘤细胞系ATM-1中发现了一个新的TCBA1基因。TCBA1在淋巴瘤中的作用目前正在研究中。

We have reported a novel gene MALT1 at chromosome 18q21 that is involved in mucosa-associated lymphoid tissue (MALT) lymphoma t(11;18)(q21;q21). This translocation results in fusion between API2 at 11q21 and MALT1 at 18q21, producing API2-MALT1 chimeric products. We are now trying to produce monoclonal antibodies against API2 and MALT1 products so that we can examine MALT lymphoma cases with immunohistochemial method.We have studied diffuse large B cell lymphoma(DLBCL) that was known to contain several subtypes although consensus for subtype classifications is difficult to obtain among various hematopathologists. We have examined BCL1, BCL2, BCL6 gene rearrangement as well as immunostaining. We found that cell surface markers could serve as markers depicting clinically significant subtypes. Indeed, CD5+ DLBCL was found to consist of a group with poor prognosis which is as worse as mantle cell lymphoma.Translocation junction genes that are involved in T-cell lymphoma are poorly understood. Based on the reports that T-cell lymphoma had frequent chromosome aberration at 6q21, we attempted to isolate the responsible genes. We identified a novel TCBA1 gene that is altered in T-cell lymphoblastoid lymphoma cell line HT-1 and adult T-cell leukemia/lymphoma cell line ATM-1. The role of TCBA1 for lymphomageneis is now under investigation.