Establishment of functional analysis for genes involved in NK cell tumors using normal NK cell expansion method

使用正常 NK 细胞扩增方法建立 NK 细胞肿瘤相关基因的功能分析

• 批准号: 23659194
• 负责人: SETO Masao
• 金额: $ 2.5万
• 依托单位: 愛知県がんセンター(研究所)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659194/
• 关键词: 血液; 悪性リンパ腫; NK細胞; ゲノム異常

In an attempt to clarify molecular mechanisms of NK cell tumor development, oligo-array CGH and expression profiling were applied. Oligo-array CGH identified two distinct minimal common deleted regions at 6q21 where the most frequent deletion was observed at the percentage of 36%(14 of 39 cases). The genes identified were PRDM1 and FOXO3 which were functionally approved by inducing expression system. Normal NK cells were know to be expanded by culturing peripheral blood mononuclear cells(PBMC) with irradiated K562-mb15-41BBL cells to one million order of magnitude. We could amplified normal NK cells to the same order of magnitude with feeder cells recovered from frozen status. This system will be useful for the functional analysis of genes involved in NK cell tumor development.

为了阐明NK细胞肿瘤发展的分子机制，应用寡核苷酸阵列CGH和表达谱。寡核苷酸阵列CGH确定了两个不同的最小共同缺失区域在6 q21，其中最常见的缺失观察到的百分比为36%（14/39例）。经诱导表达系统鉴定，PRDM 1和FOXO 3基因在功能上得到了证实。已知正常NK细胞通过用辐照的K562-mb 15 - 41 BBL细胞培养外周血单核细胞（PBMC）扩增至一百万个数量级。我们可以将正常NK细胞与从冷冻状态恢复的饲养细胞扩增到相同的数量级。该系统将有助于NK细胞肿瘤发生相关基因的功能分析。

项目成果

Promotion and maintenance of leukemia by ERG

• DOI: 10.1182/blood-2010-11-320515
• 发表时间: 2011-04-07
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Tsuzuki, Shinobu;Taguchi, Osamu;Seto, Masao
• 通讯作者: Seto, Masao

Gene Expression Profiling of Age-Related Epstein-Barr Virus(EBV)-Associated B-Cell Lymphoproliferative Disorder Uncovers Alterations in Immune andInflammatory Genes : Possible Implications for Pathogenesis

年龄相关的 Epstein-Barr 病毒 (EBV) 相关 B 细胞淋巴细胞增殖性疾病的基因表达谱揭示了免疫和炎症基因的改变：对发病机制的可能影响

• 发表时间: 2011
• 作者: Harumi Kato;Kazuhito Yamamoto;Masao Seto
• 通讯作者: Masao Seto

Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses

• DOI: 10.1182/blood-2011-04-346890
• 发表时间: 2011-09-22
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Karube, Kennosuke;Nakagawa, Masao;Seto, Masao
• 通讯作者: Seto, Masao

Altered differentiation and enhanced self-renewal activity of B cells by the leukemia-associated TEL(ETV6)-AML1(RUNX1) fusion gene

白血病相关 TEL(ETV6)-AML1(RUNX1) 融合基因改变 B 细胞的分化并增强自我更新活性

• 发表时间: 2011
• 作者: Masaharu Tashima;Masao Seto(他6名;8番目);Masaharu Tashima;加留部謙之輔,中川雅夫,瀬戸加大;加留部謙之輔;加藤春美,山本一仁,瀬戸加大;瀬戸加大;加藤春美;松浦恵子,中田知里,瀬戸加大;成松隆弘,松浦恵子,瀬戸加大;都築忍,瀬戸加大
• 通讯作者: 都築忍,瀬戸加大

Identification of FOXO3 and PRDM1 as tumor suppressor gene candidates in NK cell neoplasms by the combination of genomic and functional analyses

通过基因组和功能分析相结合，鉴定 FOXO3 和 PRDM1 作为 NK 细胞肿瘤中的候选抑癌基因

• 发表时间: 2011
• 作者: Kennosuke Karube;Masao Nakagawa;Masao Seto
• 通讯作者: Masao Seto