Establishment of functional analysis for genes involved in NK cell tumors using normal NK cell expansion method

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In an attempt to clarify molecular mechanisms of NK cell tumor development, oligo-array CGH and expression profiling were applied. Oligo-array CGH identified two distinct minimal common deleted regions at 6q21 where the most frequent deletion was observed at the percentage of 36%(14 of 39 cases). The genes identified were PRDM1 and FOXO3 which were functionally approved by inducing expression system. Normal NK cells were know to be expanded by culturing peripheral blood mononuclear cells(PBMC) with irradiated K562-mb15-41BBL cells to one million order of magnitude. We could amplified normal NK cells to the same order of magnitude with feeder cells recovered from frozen status. This system will be useful for the functional analysis of genes involved in NK cell tumor development.