Functional change of exudative polymorphonuclear neutrophil in surgical stress.

我们研究了营养不良对手术应激中中性粒细胞多形核中性粒细胞（PMN）介导免疫的影响。实验结果如下：雄性C57BL/6J小鼠204只，随机分为自由饮食组、中等限制饮食组和重度限制饮食组，分别给予小鼠自由饮食（132 g/kg / d）、66 g/kg / d、33 g/kg / d，连续7 d。在饮食限制后，再饲喂/不饲喂1天，小鼠给予糖原i.p.p诱导细胞渗出。糖原安装后，检测循环PMNs中CD11b、CD18和CD62L的表达、渗出PMNs的吞噬作用和ROI的产生。同时检测PLF中PMN特异性趋化因子、巨噬细胞炎症蛋白(MIP)-2水平。1)限制饮食7 d可降低循环PMN中CD11b/CD18的表达；2)限制饮食7 d可减少腹膜炎早期PMN向腹腔的渗出。3)循环PMNs中CD11b和CD18的表达均与渗出细胞数量显著相关。4) 7天的饮食限制也使吞噬功能受损5)7天的饮食限制上调了渗出性pmn产生的ROI。6)饮食限制7 d可降低PLF患者的MIP-2水平。

We investigated thc influence of malnutrition on neutrophil Polymorphonuclear neutrophil (PMN)-mediated immunity in surgical stress. Obtained results are as follows : Male C57BL/6J mice (n=204) were assigned to ad libitum, moderate and severe dietrestricted groups receiving mouse chow ad libitum (132 g/kg per day), 66 and 33 g/kg per day for seven days, respectively. After dietary restriction with/without one day of refeeding, mice were administered glycogen i.p.to induce cell exudation. CD11b CD18 and CD62L expressions on circulating PMNs, phagocytosis and ROI production by exudative PMNs, were measured after glycogen installation.The levels of PMN specific chemokine, macrophage inflammatory protein (MIP)-2 in PLF were also measured.1)Seven days of dietary restriction decreased CD11b/CD18 expression on circulating PMNs,2) Seven days of dietary restriction decreased subsequent PMN exudation into the peritoneal cavity early in peritonitis.3)Both CD11b and CD18 expression on circulating PMNs, correlated significantly with numbers of exudative.4) Seven days of dietary restriction also impaired phagocytosis5) Seven days of dietary restriction upregulated ROI production by exudative PMNs.6)Seven days of dietary restriction decreased MIP-2 levels in PLF.