Functional change of exudative polymorphonuclear neutrophil in surgical stress.

手术应激中渗出性多形核中性粒细胞的功能变化。

• 批准号: 11470240
• 负责人: SAITO Hideaki
• 金额: $ 8万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470240/
• 关键词: neutrophil; malnutrition; adhesion molecule; Exudation; phagocytosis; ROI production; cytokines; 貧食能; 活性酸素産出能

We investigated thc influence of malnutrition on neutrophil Polymorphonuclear neutrophil (PMN)-mediated immunity in surgical stress. Obtained results are as follows : Male C57BL/6J mice (n=204) were assigned to ad libitum, moderate and severe dietrestricted groups receiving mouse chow ad libitum (132 g/kg per day), 66 and 33 g/kg per day for seven days, respectively. After dietary restriction with/without one day of refeeding, mice were administered glycogen i.p.to induce cell exudation. CD11b CD18 and CD62L expressions on circulating PMNs, phagocytosis and ROI production by exudative PMNs, were measured after glycogen installation.The levels of PMN specific chemokine, macrophage inflammatory protein (MIP)-2 in PLF were also measured.1)Seven days of dietary restriction decreased CD11b/CD18 expression on circulating PMNs,2) Seven days of dietary restriction decreased subsequent PMN exudation into the peritoneal cavity early in peritonitis.3)Both CD11b and CD18 expression on circulating PMNs, correlated significantly with numbers of exudative.4) Seven days of dietary restriction also impaired phagocytosis5) Seven days of dietary restriction upregulated ROI production by exudative PMNs.6)Seven days of dietary restriction decreased MIP-2 levels in PLF.

我们研究了营养不良对手术应激中中性粒细胞多形核中性粒细胞（PMN）介导免疫的影响。实验结果如下：雄性C57BL/6J小鼠204只，随机分为自由饮食组、中等限制饮食组和重度限制饮食组，分别给予小鼠自由饮食（132 g/kg / d）、66 g/kg / d、33 g/kg / d，连续7 d。在饮食限制后，再饲喂/不饲喂1天，小鼠给予糖原i.p.p诱导细胞渗出。糖原安装后，检测循环PMNs中CD11b、CD18和CD62L的表达、渗出PMNs的吞噬作用和ROI的产生。同时检测PLF中PMN特异性趋化因子、巨噬细胞炎症蛋白(MIP)-2水平。1)限制饮食7 d可降低循环PMN中CD11b/CD18的表达；2)限制饮食7 d可减少腹膜炎早期PMN向腹腔的渗出。3)循环PMNs中CD11b和CD18的表达均与渗出细胞数量显著相关。4) 7天的饮食限制也使吞噬功能受损5)7天的饮食限制上调了渗出性pmn产生的ROI。6)饮食限制7 d可降低PLF患者的MIP-2水平。

项目成果

Ikeda S., Saito H., Hidemura A., Fukatsu K.: "Dietrestriction reduces chemokine production and exudation of polymorphonuclear neutrophils at local inflammatory sites"Clinical Nutrition. 19(Supplement1). 15 (2000)

Ikeda S.、Saito H.、Hidemura A.、Fukatsu K.：“饮食限制减少局部炎症部位趋化因子的产生和多形核中性粒细胞的渗出”临床营养。

池田 重雄 他: "腹腔内炎症における局所滲出細胞の動態-滲出細胞数とその機能の変動"外科と代謝・栄養. 33(3). 141-141 (1999)

Shigeo Ikeda 等人：“腹膜内炎症中局部渗出细胞的动态 - 渗出细胞数量及其功能的波动”，Surgery、Metabolism and Nutrition 33(3) (1999)。

S.Ikeda,et al.: "Malnutrition Impairs Adhesion and Subsequent Migration of Polymorphonuclear Neutrophils In Vitro"JPEN. 24(1). S3-S4 (2000)

S.Ikeda 等人：“营养不良会损害体外多形核中性粒细胞的粘附和随后的迁移”JPEN。

S.Ikeda,et al.: "Effects of Malnutrition on Exudation and Function of Polymorph nuclear Neutrophils at Inflammatory Site"JPEN. 23(1). S15-S15 (1999)

S.Ikeda等：“营养不良对炎症部位多形核中性粒细胞渗出和功能的影响”JPEN。

S.Ikeda,et al.: "Malnutrition impairs exudation and function of polymorphonuclear neutrophil at inflammatory site"Clinical Nutrition. 18(1). 6-6 (1999)

S.Ikeda 等人：“营养不良会损害炎症部位多形核中性粒细胞的渗出和功能”临床营养。