MODULATION OF NEURO-ENDOCRINE-IMMUNE RESPONSES IN SURGICAL STRESS

手术应激中神经内分泌免疫反应的调节

• 批准号: 07457246
• 负责人: SAITO Hideaki
• 金额: $ 4.67万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457246/
• 关键词: SURGICAL STRESS; CYTOKINES; BIOLOGICAL RESPONSES; HOST DEFENSE; TNF; IL-1; SURGICAL NUTRITION; NITRIC OXIDE

The purpose of this research was how to modulate neruo-endocrine-immune responses in surgical stress. The result is as follows :1. Nutritional routes and glutamine supplementation : Both antecedent enteral nutrition and Ala-Gln enriched TPN enhance local, systemic and remote organ immune responses to intraperitoneal bacterial chalenge. On the basis of our findings, critical illness whenever the enteral route is feasible. Ala-Gln enriched TPN may be an option for enhancing host defense when enteral nutrition can not be tolerated.2. Growth hormone and Insulin-like growth factor 1 : GH and IGF-1 enhance phagocytosis and the E.coli-killing activity of PECs, via a modestly increased plasma capacity to support these activities, as well as by a strong direct action.3. Nitric oxidase inhibitor and NO donor : Administration of L-NAME increased neutrophil adhesion in the lung, while decreasing that in the peritoneum. NO inhibition may be ditrimental, due to neutrophil sequestration, in this peritonitis model. NO donor administration prevented neutrophil adhesion in the lung, but decreased neutrophil adhesion in the peritoneal cavity.

本研究的目的是探讨手术应激对机体神经内分泌免疫功能的影响。研究结果如下：1.营养途径和谷氨酰胺补充：预先肠内营养和富含Ala-Gln的TPN均可增强对腹膜内细菌挑战的局部、全身和远端器官免疫应答。根据我们的研究结果，只要肠内途径可行，危重病。结论：1.当肠内营养不能耐受时，富含Ala-Gln的TPN可作为增强机体防御能力的一种选择.生长激素和胰岛素样生长因子1：GH和IGF-1通过适度增加血浆支持这些活动的能力以及强烈的直接作用，增强佩奇的吞噬作用和大肠杆菌杀灭活性。3.一氧化氮氧化酶抑制剂和NO供体：L-NAME的给药增加了肺中的中性粒细胞粘附，同时减少了腹膜中的中性粒细胞粘附。在该腹膜炎模型中，由于中性粒细胞隔离，NO抑制可能是双效的。NO供体给药可防止肺中的中性粒细胞粘附，但可减少腹膜腔中的中性粒细胞粘附。

项目成果

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Liu M, Saito, H 等人：“The Immune Cousegreeces of Trans, Shock and Sepsis. Mechionics and thwbaoeetic approches vol” PABST Science Publick, 886-888 (1996)

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Fukutsu,K,Saito P 等人：“分裂 enveuterowutw 是 veuhophil exuclatin no peitoritis 的 puuey 位点”J.Am.Cell.Sug.183 (1996)。