Signal transduction in neutrophils under surgical stress

手术应激下中性粒细胞的信号转导

• 批准号: 12557098
• 负责人: SAITO Hideaki
• 金额: $ 8.77万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557098/
• 关键词: neutrophil; macrophage; malnutrition; signal transduction; thyrosine kinase; host immunity

Malnutrition impairs host immunity resulting in high mortality and morbidity due to infections. Phosphorylation of protein tyrosine kinase (PTK) is a key step in the signaling of many cellular functions. Malnutrition may affect this signaling in response to surgical insults. The aim of this study was to examine effects of PTK inhibition on host immunity.Diet restriction reduced survival of mice after cecal ligation and puncture compared with ad libitum group. Tyrosine kinase inhibition decreased survival of ad libitum mice but did not change that of diet restricted mice. Tyrosine phosphorylation in peritoneal exudative cells from mice with malnutrition was inhibited after stimulation, which may be an important mechanism for impaired host immunity accompanying to malnutrition.

营养不良损害宿主免疫力，导致感染引起的高死亡率和发病率。蛋白酪氨酸激酶（PTK）的磷酸化是许多细胞功能信号传导的关键步骤。营养不良可能会影响这种信号在响应手术侮辱。本研究的目的是检测PTK抑制对宿主免疫的影响。与自由采食组相比，饮食限制降低了盲肠结扎和穿孔后小鼠的存活率。酪氨酸激酶抑制降低了自由活动小鼠的存活率，但没有改变饮食限制小鼠的存活率。营养不良小鼠腹腔渗出液细胞酪氨酸磷酸化在刺激后受到抑制，这可能是营养不良导致宿主免疫功能受损的重要机制。

项目成果

Matsuda T, Saito H, Fukatsu K, Han I, Inoue T, Furukawa S, Ikeda S and Hidemura A: "Cytokine-modulated inhibition of neutrophil apoptosis at local site augments exudative neutrophil functions and reflects inflammatory response after surgery"Surgery. 129.

Matsuda T、Saito H、Fukatsu K、Han I、Inoue T、Furukawa S、Ikeda S 和 Hidemura A：“局部部位细胞因子调节的中性粒细胞凋亡抑制可增强渗出性中性粒细胞功能并反映手术后的炎症反应”。

Lin MT SH, Furukawa S, Fukushima R, Kazuhiko F, Lee PH, Chang KJ, Chen WJ.: "Alanyl-glutamine enriched total parenteral nutrition improves local, systemic, and remote organ responses to intraperitoneal bacterial challenge"JPEN J Parenter Enteral Nutr.. 25

Lin MT SH、Furukawa S、Fukushima R、Kazuhiko F、Lee PH、Chang KJ、Chen WJ.：“富含丙氨酰谷氨酰胺的全肠外营养可改善局部、全身和远程器官对腹膜内细菌挑战的反应”JPEN J 肠外营养品

Kang W, Sito h, Fukatsu K, Hidemura A, Koyama H, Sakamoto T, Maekawa K: "Effects of tyrosine kinase signaling inhibition on survival after cecal ligation and puncture in diet-restricted mice"JPEN Journal of Parenteral Enteral Nutriton. 25(6). 291-297 (200

Kang W、Sito h、Fukatsu K、Hidemura A、Koyama H、Sakamoto T、Maekawa K：“酪氨酸激酶信号传导抑制对饮食限制小鼠盲肠结扎和穿刺后生存的影响”JPEN 肠外肠内营养杂志。

Lin MT, Saito H, Furukawa S, Fukushima R, Fukatsu K, Lee PH, Chang KJ, Chen WJ: "Alanyl-glutamine enriched total parenteral nutrition improves local, systemic and remote organ responses to intraperitoneal bacterial challenge"JPEN Journal of Parenteral Ent

Lin MT、Saito H、Furukawa S、Fukushima R、Fukatsu K、Lee PH、Chang KJ、Chen WJ：“富含丙氨酰谷氨酰胺的全肠外营养改善局部、全身和远程器官对腹膜内细菌挑战的反应”JPEN 肠外耳鼻喉科杂志

Han I, Saito H, Fukatsu K, Inoue T, Yasuhara H, Furukawa S, Matsuda T, Lin MT, Ikeda S: "Ex vivo fluorescence microscopy provides simple and accurate assessment of neutrophii-endothelial adhesion in the rat lung"Shock. 16(2). 143-147 (2001)

Han I、Saito H、Fukatsu K、Inoue T、Yasuhara H、Furukawa S、Matsuda T、Lin MT、Ikeda S：“体外荧光显微镜提供了对大鼠肺中性粒细胞内皮粘附的简单而准确的评估”休克。