Signal transduction in neutrophils under surgical stress

营养不良损害宿主免疫力，导致高死亡率和感染致残率。蛋白酪氨酸激酶(PTK)的磷酸化是许多细胞功能信号传递的关键步骤。营养不良可能会影响这种信号对外科手术侮辱的反应。本研究的目的是检测抑制PTK对宿主免疫的影响。与随意组相比，饮食限制降低了盲肠结扎和穿孔后小鼠的存活率。抑制酪氨酸激酶降低了自由饮食小鼠的存活率，但不改变饮食限制小鼠的存活率。营养不良小鼠腹膜渗出细胞在刺激后酪氨酸磷酸化受到抑制，这可能是营养不良患者机体免疫功能受损的重要机制之一。

Malnutrition impairs host immunity resulting in high mortality and morbidity due to infections. Phosphorylation of protein tyrosine kinase (PTK) is a key step in the signaling of many cellular functions. Malnutrition may affect this signaling in response to surgical insults. The aim of this study was to examine effects of PTK inhibition on host immunity.Diet restriction reduced survival of mice after cecal ligation and puncture compared with ad libitum group. Tyrosine kinase inhibition decreased survival of ad libitum mice but did not change that of diet restricted mice. Tyrosine phosphorylation in peritoneal exudative cells from mice with malnutrition was inhibited after stimulation, which may be an important mechanism for impaired host immunity accompanying to malnutrition.