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An experimental study about anti-local inflammation effects of liquid ventilation.

液体通气抗局部炎症作用的实验研究。

基本信息

批准号：
11470273
负责人：
SHIMIZU Nobuyoshi
金额：
$ 8.7万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2002
项目状态：
已结题

项目摘要

The objectives of this study is to evaluate the effect of partial liquid ventilation (PLV) with respect to TNF-_a, MIP-2, CINC-1 concentration in BAL in LPS induced acute lung injury model in rats, and the surface expression of CD11b and macrophage subset as well. This study was designed as experimental, prospective, controlled study. Thirty-four rats assigned to three groups. The 30 minutes group was treated with gas ventilation for 30 minutes after intratracheal LPS administration ; the GV group was treated with 30 minutes of gas ventilation after LPS administration, then gas ventilation was continued for following 2 hours ; and the PLV group was treated with 30 minutes of gas ventilation after LPS administration, then PLV with Perflubron was performed for following 2 hours. At the end of each ventilations, animals were euthanized and BAL was performed. The concentrations of TNF α, MIP-2, CINC-1 were measured in BAL supernatant by ELISA. There were no significant difference between GV and PLV group in TNF α (GV : 18828.7 ± 10676.6, PLV : 20008.1 ± 1573.8 which represents mean ± SEM), MIP-2 (13141.2 ± 943.6, PLV : 14433.5 ± 147.0), and CINC-1 (GV : 8765.2 ± 1129.1, PLV : 10144.4 ± 1220.7). The effects of PLV on cell surface e pression of CD11b in neutrophils, and that of anti macrophage subset were determined by the flow cytometric analysis in BAL cells. There was no significant difference of positive expression ratio (positive cells / aqurired cells) in CD11b (GV : 63.6 ± 8.4, PLV : 60.5 ± 5.4). However macrophage expression ratio was significantly increased (GV : 5.6 ± 1.5, PLV : 14.0 ± 1.3, p<0.005). BAL supernatant TNF α, MIP-2, CINC-1 concentration is not affected significantly by PLV with perflubron in this rodent model of LPS induced acute lung injury, nor CD11b cell surface expression. However the ratio of macrophage per unit in BAL cells was increased by PLV.

本研究的目的是评价部分液体通气（PLV）对脂多糖（LPS）诱导的大鼠急性肺损伤模型中肺泡灌洗液（BAL）中TNF-a、MIP-2、CINC-1浓度以及CD 11b和巨噬细胞亚群表面表达的影响。本研究为实验性、前瞻性、对照研究。将34只大鼠分为3组。30 min组在注射LPS后进行气体通气30 min; GV组在注射LPS后进行气体通气30 min，然后继续气体通气2 h; PLV组在注射LPS后进行气体通气30 min，然后进行Perflubron PLV 2 h。在每次通气结束时，对动物实施安乐死并进行BAL。ELISA法检测BALF上清液中TNF α、MIP-2、CINC-1的含量。GV组与PLV组TNF α水平无显著性差异（GV：18828.7 ± 10676.6，PLV：20008.1 ± 1573.8，代表平均值± SEM），MIP-2（13141.2 ± 943.6，PLV：14433.5 ± 147.0）和CINC-1（GV：8765.2 ± 1129.1，PLV：10144.4 ± 1220.7）。用流式细胞仪检测PLV对中性粒细胞表面CD 11b表达的影响，以及对BAL细胞抗巨噬细胞亚群的影响。CD 11b的阳性表达率（阳性细胞/培养细胞）GV：63.6 ± 8.4，PLV：60.5 ± 5.4，两组间差异无统计学意义。而巨噬细胞表达率显著增加（GV：5.6 ± 1.5，PLV：14.0 ± 1.3，p<0.005）。在LPS诱导的急性肺损伤的啮齿动物模型中，PLV与perflubron对BAL上清液TNF α、MIP-2、CINC-1的浓度没有显著影响，也不影响CD 11b细胞表面表达。但PLV可增加BAL细胞中单位巨噬细胞的比例。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

庄賀一彦,小森栄作,青江基,市場晋吾,安藤陽夫,清水信義: "ラットLPS気道内投与呼吸不全モデルにおける部分液体呼吸の抗炎症効果の検討"日本呼吸器外科学会雑誌. 14巻・3号. 239-239 (2000)

Kazuhiko Shogga、Eisaku Komori、Hajime Aoe、Shingo Ichiba、Haruo Ando、Nobuyoshi Shimizu：“在气管内给予 LPS 的呼吸衰竭大鼠模型中部分液体呼吸的抗炎作用的检查”日本学会杂志呼吸外科杂志，第 14 卷，第 3 期，第 239-239 号（2000 年）

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通讯作者：

小森栄作,庄賀一彦,青江基,佐野由文,市場晋吾,清水信義,木村五郎: "ラット気道LPS投与呼吸不全モデルにおけるPartial Liquid Ventilationの抗炎症効果の検討"日本呼吸器学会雑誌. 38巻・増刊号. 132-132 (2000)

Eisaku Komori、Kazuhiko Shuga、Hajime Aoe、Yoshifumi Sano、Shingo Ichiba、Nobuyoshi Shimizu、Goro Kimura：“在使用气道 LPS 的大鼠呼吸衰竭模型中部分液体通气的抗炎作用的检查” 日本呼吸学会杂志.第38卷·特刊（2000年）。