An experimental study about anti-local inflammation effects of liquid ventilation.

本研究旨在探讨部分液体通气（PLV）对内毒素（LPS）诱导的大鼠急性肺损伤（ALI）模型肺泡灌洗液（BAL）中TNF-α、MIP-2、CINC-1浓度及肺泡表面CD 11b表达和巨噬细胞亚群的影响。本研究为实验性、前瞻性、对照研究。将34只大鼠分为3组。30 min组在注射LPS后进行气体通气30 min; GV组在注射LPS后进行气体通气30 min，然后继续气体通气2 h; PLV组在注射LPS后进行气体通气30 min，然后进行Perflubron PLV 2 h。在每次通气结束时，对动物实施安乐死并进行BAL。ELISA法检测BALF上清液中TNF α、MIP-2、CINC-1的含量。GV组与PLV组TNF α水平无显著性差异（GV：18828.7 ± 10676.6，PLV：20008.1 ± 1573.8，代表平均值± SEM），MIP-2（13141.2 ± 943.6，PLV：14433.5 ± 147.0）和CINC-1（GV：8765.2 ± 1129.1，PLV：10144.4 ± 1220.7）。用流式细胞仪检测PLV对中性粒细胞表面CD 11b表达的影响，以及对BAL细胞抗巨噬细胞亚群的影响。CD 11b的阳性表达率（阳性细胞/培养细胞）GV：63.6 ± 8.4，PLV：60.5 ± 5.4，两组间差异无统计学意义。而巨噬细胞表达率显著增加（GV：5.6 ± 1.5，PLV：14.0 ± 1.3，p<0.005）。在LPS诱导的急性肺损伤的啮齿动物模型中，PLV与perflubron对BAL上清液TNF α、MIP-2、CINC-1的浓度没有显著影响，也不影响CD 11b细胞表面表达。但PLV可增加BAL细胞中单位巨噬细胞的比例。

The objectives of this study is to evaluate the effect of partial liquid ventilation (PLV) with respect to TNF-_a, MIP-2, CINC-1 concentration in BAL in LPS induced acute lung injury model in rats, and the surface expression of CD11b and macrophage subset as well. This study was designed as experimental, prospective, controlled study. Thirty-four rats assigned to three groups. The 30 minutes group was treated with gas ventilation for 30 minutes after intratracheal LPS administration ; the GV group was treated with 30 minutes of gas ventilation after LPS administration, then gas ventilation was continued for following 2 hours ; and the PLV group was treated with 30 minutes of gas ventilation after LPS administration, then PLV with Perflubron was performed for following 2 hours. At the end of each ventilations, animals were euthanized and BAL was performed. The concentrations of TNF α, MIP-2, CINC-1 were measured in BAL supernatant by ELISA. There were no significant difference between GV and PLV group in TNF α (GV : 18828.7 ± 10676.6, PLV : 20008.1 ± 1573.8 which represents mean ± SEM), MIP-2 (13141.2 ± 943.6, PLV : 14433.5 ± 147.0), and CINC-1 (GV : 8765.2 ± 1129.1, PLV : 10144.4 ± 1220.7). The effects of PLV on cell surface e pression of CD11b in neutrophils, and that of anti macrophage subset were determined by the flow cytometric analysis in BAL cells. There was no significant difference of positive expression ratio (positive cells / aqurired cells) in CD11b (GV : 63.6 ± 8.4, PLV : 60.5 ± 5.4). However macrophage expression ratio was significantly increased (GV : 5.6 ± 1.5, PLV : 14.0 ± 1.3, p<0.005). BAL supernatant TNF α, MIP-2, CINC-1 concentration is not affected significantly by PLV with perflubron in this rodent model of LPS induced acute lung injury, nor CD11b cell surface expression. However the ratio of macrophage per unit in BAL cells was increased by PLV.