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Development of Simple Denitrification System With Autotrophic Bacteria for Developing Countries

为发展中国家开发简单的自养细菌反硝化系统

基本信息

批准号：
11480151
负责人：
HANO Tadashi
金额：
$ 4.16万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480151/
关键词：
Developing Countries Simplification Denitrification Autotrophic Bacteria Sulfur Thiobacuius denitrificans

项目摘要

A simple denitrification process suitable for developing countries was proposed by employing autotrophic Thiobacillus dentrificans. The effect of nitrate concentration, phosphorus concentration, alkalinity and inhibitants on the rate of denitrification was investigated with a column packed with sulfur and limestone particles involving denitrification bactaeria, and the following results were obtained.1) The denitrification proceeded without substrate inhibition up to 1,000 g-nitrate-N/m^3, which demonstrated that most of the wastewater cold be treated without dilution.2) The minimum phosphorus concentration was evaluated under constant pH controlled by borate-buffer showing that 1 mg-P/m^3 was enough for the present process. However, higher phosphorus concentration was necessary when borate buffer was not used. This was because pH at the reaction site, probably the boundary between biofilm and surface of sulfur particles, may drop due to the release of proton as a result of denitrification.3) The minimum alkalinity was found as 600g-CaCO_3/m^3 for load of 420g-N/(m^3/d). The inhibition with fluoride and Ni was not observed until 90 and 3 g/m^3, respectively.It was concluded that the present system was suitable for developing countries because it was simple without any special instruments or technical care and it offered stable performance with slight request for phosphorus even in concentrated wastewater.

提出了一种适用于发展中国家的简单脱氮工艺，即采用自养型脱氮硫杆菌脱氮。采用硫和石灰石颗粒填充的反硝化细菌柱，研究了硝酸盐浓度、磷浓度、碱度和澄清剂对反硝化速率的影响，得到以下结果：1）反硝化在1,000 g-硝酸盐-N/m^3以下无底物抑制，2）在硼酸盐缓冲液控制的恒定pH条件下，测定了最低磷浓度，结果表明1 mg-P/m^3的最低磷浓度足以满足本工艺的要求。然而，当不使用硼酸盐缓冲液时，需要更高的磷浓度。这是因为在反应位点，可能是生物膜和硫颗粒表面之间的边界，由于质子的释放，pH值可能会下降。3）在负荷为420 g-N/（m^3/d）时，最低碱度为600 g-CaCO_3/m^3。氟化物和镍的抑制作用分别在90和3 g/m^3时才观察到。得出的结论是，本系统适用于发展中国家，因为它简单，无需任何特殊的仪器或技术维护，即使在浓废水中也能提供稳定的性能，对磷的要求很低。