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Development of Lactic Acid Fermentation Process Accompanied with Selective Product Separation

伴随选择性产物分离的乳酸发酵工艺的开发

基本信息

批准号：
08555195
负责人：
HANO Tadashi
金额：
$ 5.44万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

The fermentative production process of L-lactic and as a raw material of biodegradable polymer was developed by employing Lactobacillus rhamnosus and Rhizopus oryzae and the following results were obtained concerning the fermentation and products separation.1) In the extractive fermentation with Lactobacillus rhamnosus, it became possible to remove completely the dissolved extractant which is toxic for microbes by using a cleaning column packed with cation exchange resin. The product inhibition, which severely retarded the fermentation under the initial glucose concentration of 100g/L, was suppressed with the present extractive fermentation system and fermentation proceeded satisfactorily.2) In the electrodialysis fermentation with Lactobacillus rhamnosus, a continuous fermentation method utilizing the permeation of water was found to give a stable production of lactic acid for long term. By using a glucose sensor in a fermentor, it was possible to keep the concentration of glucose at low level and to increase the yield of lactic acid.3) In the fermentation with Rhizopus oryzae, the addition of activated carbon to the fermentation broth was very effective in increasing the productivity and yield and obtaining clean broth which simplified the downstream processing.4) The lactic acid production systems investigated in this project were evaluated. It was concluded that an extractive fermentation with Lactobacillus rhamnosus was suitable for a small-scale batch operation whereas an electrodialysis fermentation with the same bacteria was for a small-scale continuous operation. Rhizopus oryzae was suitable for a large-scale fermentation.

以鼠李糖乳杆菌和米根霉为原料，开发了l -乳酸和生物可降解聚合物的发酵生产工艺，并在发酵和产物分离方面取得了以下结果。1)在鼠李糖乳杆菌的萃取物发酵过程中，采用阳离子交换树脂填充的净化柱，可以完全去除溶解的对微生物有毒害的萃取物。在初始葡萄糖浓度为100g/L的条件下，产物抑制作用严重阻碍了发酵，该提取发酵体系抑制了产物抑制作用，发酵效果良好。2)在鼠李糖乳杆菌的电渗析发酵中，发现了一种利用水渗透的连续发酵方法，可以长期稳定地生产乳酸。通过在发酵罐中使用葡萄糖传感器，可以将葡萄糖的浓度保持在较低的水平，并提高乳酸的产量。3)在米根霉发酵过程中，在发酵液中添加活性炭可有效提高生产效率和产量，获得干净的发酵液，简化了下游工序。4)对本项目研究的乳酸生产体系进行了评价。结果表明，鼠李糖乳杆菌提取发酵适合小规模批量生产，而电渗析发酵适合小规模连续生产。米根霉适合大规模发酵。