Preparation of biomedical materials by the optimum hybridization of self-assembling polymeric nanospheres and biopolymers

自组装聚合物纳米球与生物聚合物优化杂交制备生物医用材料

• 批准号: 11480259
• 负责人: AKASHI Mitsuru
• 金额: $ 7.17万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480259/
• 关键词: polymeric nanospheres; self-assembling; biopolymers; peptides as medicine; carbohydrates; phospholipids; lectin; HIV vaccine; エイズワクチン; 高分子微粒子; 糖鎖; タンパク質; レクチン; ポリスチレン; ポリスチレンナノスフェア; 糖鎖固定化; グリボトリオース固定化; ベロ毒素補足; レクチン固定化; HIV-1捕捉

Free radical dispersion copolymerization of hydrophobic monomers with hydrophilic macromonomers in a polar solvent without any emulsifier gives monodispersed polymeric nanospheres, and the nanosphere formation mechanism based on self-assembling of multiphase polymers suggests that hydrophilic macromonomer chains cover the surface of the nanospheres to reduce core-corona polymeric nanospheres. Using the macromonomer method, a variety of core-corona polymeric nanospheres can be prepared and their surface can be easily modified by macromonomer chains.In this study, biopolymers or biomolecules such as (1) peptides as medicine, (2) carbohydrates for the recognition of proteins, (3) phospholipids analog for giving a bioinspired polymer surface, and (4) lectin and HTV-1 for capturing HIV-1 and HIV-1 vaccine, respectively, were immobilized on the corona of polymeric nanospheres. We concluded that the optimum hybridization of self-assembling polymeric nanospheres and biopolymers is a key of highly functional particle preparation and important for design of biomedical materials.

疏水大分子单体与亲水性大分子单体在无乳化剂的极性溶剂中自由基分散共聚得到单分散的聚合物纳米球，基于多相聚合物自组装的纳米球形成机理表明，亲水性大分子单体链覆盖在纳米球表面以减少核-晕聚合物纳米球的形成。采用大单体法制备了多种核冠状聚合物纳米球，其表面容易被大分子链修饰。本研究将生物聚合物或生物分子如(1)作为药物的多肽、(2)用于识别蛋白质的碳水化合物、(3)用于提供生物启发的聚合物表面的磷脂类似物、(4)用于捕获HIV-1和HIV-1疫苗的凝集素和HTV-1分别固定在聚合物纳米球的冠层上。我们认为，自组装聚合物纳米球与生物聚合物的最佳杂交是制备高功能粒子的关键，对生物医学材料的设计具有重要意义。

项目成果

Masaki Kawamura et al.: "Induction of Mucosal IgA Following Intravaginal Administration of Inactivated HIV-1-capturing Nanospheres in Mice"J. Virology. 66. 291-298 (2002)

Masaki Kawamura 等人：“在小鼠体内阴道内施用灭活的 HIV-1 捕获纳米球后诱导粘膜 IgA”J。

Takeshi Serizawa et al.: "Synthesis and Lectin Recognition of Polystyrene Core-Glycopolymer Corona Nanospheres"Biomacromolecules. 2. 469-475 (2001)

Takeshi Serizawa 等人：“聚苯乙烯核心-糖聚合物电晕纳米球的合成和凝集素识别”生物大分子。

Toshiro Uchida et al.: "Graft Copolymers Having Hydrophobic Backbone and Hydrophilic Branches XXXIV. Interaction of Polystyrene Nanospheres Having Lactose-Immobilized Hydrophilic Polymers on Their Surface and Hepatocytes"Biomacromolecules. 2. 1343-1346 (2

Toshiro Uchida等人：“具有疏水主链和亲水支链的接枝共聚物XXXIV.表面上具有乳糖固定亲水聚合物的聚苯乙烯纳米球与肝细胞的相互作用”生物大分子。

明石 満: "高分子ナノスフェアの新展開"高分子. 48. 148-152 (1999)

Mitsuru Akashi：“聚合物纳米球的新进展”Polymer 48. 148-152 (1999)。

Shinji Sakuma et al.: "Design of Nanoparticles Composed of Graft Copolymers for Oral Peptide Delivery"Advanced Drug Delivery Reviews. 47. 21-37 (2001)

Shinji Sakuma 等人：“用于口服肽递送的接枝共聚物组成的纳米颗粒的设计”高级药物递送评论。