Comparative map of mammalian chromosomes for research on domestic and laboratory animals

用于家养动物和实验动物研究的哺乳动物染色体比较图

• 批准号: 11490024
• 负责人: SATO Katsunori
• 金额: $ 7.87万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11490024/
• 关键词: Comparative map; Linkage map; Radiation hybrid mapping; Hereditary disease; Cattle; Macaque; ラット

The order of loci on chromosomes is conserved among mammalian species. Therefore, accumulated information on human and mouse genome can be used for genetic analysis of other mammals including domestic and laboratory animals, if correspondence between chromosoma, maps of human mouse and other mammals is revealed. In the present study, we established comparative maps of mammalian chromosomes by constructing linkage and radiation hybrid maps using functional genes which are conserved among mammalian species. In particular, we constructed the chromosome maps of bovine chromosome 1, 6, 27, and 28, macaque chromosome 1, 2, and 11, mouse chromosome 1, 7, and 15, and rat chromosome 7, 10, and 12 in which locus responsible particular phenotypes including hereditary diseases have been localized.Consequently, we found the following evidences which demonstrate the correspondences between the chromosomes of the mammalian species.Distal region of bovine chromosome 6 is corresponding to human chromosome 4p, but the order of the loci is inverted in the middle of this region.Distal region of bovine chromosome 28 is corresponding to human chromosome 10.Order of loci was conserved between entire region of macaque chromosome and human chromosome 1.Macaque chromosome 2 had two regions which are corresponding to human chromosomes 7 and 21.Precise correspondences between rat chromosomes 7 and 12, mouse chromosomes 15 and 5, and human chromosomes 12 and 7 were revealed.These findings should be useful for identifying loci on these bovine, macaque and rat chromosomes which are responsible for phenotypes phenotypes.

染色体上的基因座顺序在哺乳动物物种中是保守的。因此，如果揭示人类小鼠和其他哺乳动物的染色体、图谱之间的对应关系，则积累的人类和小鼠基因组信息可用于其他哺乳动物（包括家养动物和实验动物）的遗传分析。在本研究中，我们通过使用哺乳动物物种之间保守的功能基因构建连锁和辐射杂交图谱来建立哺乳动物染色体的比较图谱。特别是，我们构建了牛1、6、27和28号染色体，猕猴1、2和11号染色体，小鼠1、7和15号染色体，以及大鼠7、10和12号染色体的染色体图谱，其中定位了负责包括遗传性疾病在内的特定表型的位点。因此，我们发现了以下证据，证明了人类染色体之间的对应关系。 哺乳动物物种。牛6号染色体的远端区域对应于人类4p染色体，但该区域中间的位点顺序倒置。牛28号染色体的远端区域对应于人类10号染色体。猕猴染色体的整个区域与人类1号染色体之间的位点顺序是保守的。猕猴2号染色体有两个区域对应于人类 7 号和 21 号染色体。揭示了大鼠 7 号和 12 号染色体、小鼠 15 号和 5 号染色体以及人类 12 号和 7 号染色体之间的精确对应关系。这些发现应该有助于识别这些牛、猕猴和大鼠染色体上负责表型的基因座。

项目成果

Matsushima, Y.他: "A mouse model of Waardenburg syndrome type 4 with a new spontaneous mutation of the endothelin-B receptor gene"Mamm.Genome. 13. 30-35 (2002)

Matsushima, Y. 等人：“具有内皮素 B 受体基因新自发突变的 4 型 Waardenburg 综合征小鼠模型”Mamm.Genome。 13. 30-35 (2002)

Takami, M.他: "The bovine fibroblast growth factor receptor 3 (FGFR3) gene eliminated as the locus responsible for bovine chondrodysplastic dwarfism based on mutation analysis and chromosomal mapping"Anim.Genet. (印刷中).

Takami, M. 等人：“基于突变分析和染色体定位，牛成纤维细胞生长因子受体 3 (FGFR3) 基因被消除，作为导致牛软骨发育不良性侏儒症的基因座”（Anim.Genet）。

Oikawa,T. and Sato, K.: "Variance of actual inbreeding derived from the number and variance of chromosome segment."J.Anim.Breed.Genet. 116. 467-473 (1999)

及川，T.

Sasazaki, S., Lee, E.J., Mannen, H., Kunieda, T., Sakurai, T., Yamauchi, K., Yeo, J.S., Tsuji, S.: "Phylogenic analysis of five beef cattle breeds using AFLP marker."Anim. Sci. J.. 72. 172 (2001)

Sasazaki, S.、Lee, E.J.、Mannen, H.、Kunieda, T.、Sakurai, T.、Yamauchi, K.、Yeo, J.S.、Tsuji, S.：“使用 AFLP 标记对五个肉牛品种进行系统发育分析。

Noguchi, J. 他: "A locus responsible for arrest of spermatogenesis is located on rat"Mamm. Genome. 10. 189-190 (2000)

Noguchi, J. 等人：“负责精子发生停滞的基因座位于大鼠”Mamm Genome。