Ecological diamics of marine birnavirus in marine organisms and the environments in the Uwa Sea

乌瓦海海洋生物和环境中海洋双病毒的生态学特征

• 批准号: 11490025
• 负责人: SUZUKI Satoru
• 金额: $ 7.42万
• 依托单位: Ehime University (2000)Kochi University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11490025/
• 关键词: Birnavirus; Japanese Pearl Oyster; Uwa Sea; Persistant infection; Ecology; PCR; Birnavirus; 海水; キャリア; プランクトン

This project has aimed to clarify the ecology of marine birnavirus (MABV) in Japanese Pearl Oyster and natural hosts, and the seasonal occurrence of MABV in the Uwa Sea.The infection status of MABV in Japanese Pearl Oyster was found to change between summer and winter. The virus infected in haemocytes in summer with small amount of genome and protein, and then spread in winter into the parenchymal cells. Virus detection rate by PCR in the cultured oyster appeared to be increased in winter. Also the virus detection rate in seawater increased in winter season.The phytoplankton in the Uwa Sea contained much amount of Nitzchia spp., which could not be digested by the oyster. This suggests that the feed plankton of the oyster might be deficient.MABV was firstly detected in Ayu, fresh water fish, whereas Amago salmon in close area possessed another birnavirus, IPNV.This suggests seawater is a carrier of MABV in Ayu.This project revealed ecology of MABV, infective state in the pearl oyster, mutation in various hosts.

该项目旨在阐明日本珍珠牡蛎中海洋双RNA病毒（MABV）的生态学和自然宿主，以及MABV在宇和海的季节性发生。发现日本珍珠牡蛎中MABV的感染状况在夏季和冬季之间发生变化。该病毒在夏季以少量基因组和蛋白质感染血细胞，然后在冬季传播到实质细胞中。冬季养殖牡蛎中 PCR 病毒检出率似乎有所增加。冬季海水中病毒检出率也有所增加。乌瓦海的浮游植物中含有大量菱形藻，牡蛎无法消化。这表明牡蛎的饲料浮游生物可能存在缺陷。MABV首先在淡水鱼香鱼中检测到，而附近的Amago鲑鱼则拥有另一种双RNA病毒IPNV。这表明海水是香鱼MABV的载体。该项目揭示了MABV的生态学、珍珠贝中的感染状态以及各种宿主的突变。

项目成果

Imajoh,M.,Suzuki,S.: "Apoptosis induced by a marine birnavirus in established cell lines from fish."Fish Pathology. 34. 73-79 (1999)

Imajoh，M.，Suzuki，S.：“海洋双RNA病毒在已建立的鱼类细胞系中诱导细胞凋亡。”鱼类病理学。

Kitamura, S-I.and Suzuki, S.: "Occurrence of marine birnavirus through the year in coastal seawater in the Uwa Sea."Marine Biotechnology. 2. 188-194 (2000)

Kitamura, S-I. 和 Suzuki, S.：“宇和海沿岸海水中全年海洋双RNA病毒的出现。”海洋生物技术。

Imajoh, M.and Suzuki, S.: "Apoptosis induced by a marine birnavirus in established cell lines from fish."Fish Pathology. 34. 73-79 (1999)

Imajoh, M. 和 Suzuki, S.：“海洋双病毒在已建立的鱼类细胞系中诱导细胞凋亡。”鱼类病理学。

Suzuki, S.and Nojima, M.: "Detection of a marine birnavirus in wild molluscan shellfish species from Japan."Fish Pathology. 34. 121-125 (1999)

Suzuki, S. 和 Nojima, M.：“在日本野生软体动物贝类中检测海洋双RNA病毒。”鱼类病理学。

Jung,S-J.,Kitamura,S-I.,Kawai,K.,Suzuki,S.: "Isolation of different types of birnavirus from ayu Plecoglossus altivelis and amago salmon Oncorhynchus rhodurus cultured in the same geographic area."Diseases of Aquatic Organism. 38. 87-91 (1999)

Jung，S-J.，Kitamura，S-I.，Kawai，K.，Suzuki，S.：“从同一地理区域养殖的香鱼 Plecoglossus altivelis 和阿马戈鲑鱼 Oncorhynchus rhodurus 中分离出不同类型的双RNA病毒。”水生生物疾病。