Transfer dynamics of microbial genes among various environments : to know destiny of genes responsible for drug resistance and pathogenicity in environments.

微生物基因在不同环境中的转移动态：了解环境中负责耐药性和致病性的基因的命运。

• 批准号: 14208063
• 负责人: SUZUKI Satoru
• 金额: $ 34.28万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14208063/
• 关键词: drug resistance gene; Vero toxin gene; sediment; Pseudomonas aeruginosa; gene transfer; virus like particle; tetracycline; 微生物; 遺伝子; 伝達; 薬剤耐性; 病原性; 海洋細菌; オキシテトラサイクリン; ベロ毒素; 伝達粒子

This project aims to know distribution of hazard genes such as drug resistance and pathogenicity genes in various environment and mechanisms of the gene transfer among microbes in natural ecosystem. Main research field is marine environment.The results in this project are summarized as follows.1)Tetracycline resistance genes, tet(M) and tet(S), were found in marine environmental bacteria. These genes have been reported from only terrestrial and clinical environments. This study is the first case of discovery of ribosomal protection protein genes such as tet(M) and tet(S) in marine environments.2)Novel tetracycline resistance gene tet(34) was found to be conveyed on chromosome of specific species of genus Vibrio. The tet(34) seems to be not transferred to other species.3)Vero toxin gene was detected in urban river sediment. Gene expression of the Vero toxin gene was active in sediment rather than river water, which suggests that river sediment is the place of transfer and activation of the Vero toxin gene.4)Pseudomonas aeruginosa was found to abundantly distribute in marine environment. This bacterium should be the most abundant organism in this planet and should play important role as gene transfer vector among various environments.5)Fluorescence gene lux cluster was classified some genotypes, which are suggested to be transferred and evolved in different host fish.6)Non-specific gene transfer virus-like particles were found in many bacterial species, which are vectors among natural environments.7)New method, DDCS, was developed, which should be useful to fractionate cells having different physical states.

本项目旨在了解耐药、致病性等危险基因在各种环境中的分布以及自然生态系统中微生物间基因转移的机制。主要研究领域为海洋环境。本项目的结果总结如下：1)在海洋环境细菌中发现tet(M)和tet(S)耐药基因。据报道，这些基因仅来自陆地和临床环境。本研究是首次在海洋环境中发现tet(M)和tet(S)等核糖体保护蛋白基因。2)在特定弧菌属的染色体上发现了新的四环素耐药基因tet（34）。tet（34）似乎没有转移到其他物种。3)城市河流沉积物中检测到Vero毒素基因。Vero毒素基因在沉积物中表达较活跃，而在河水中表达较活跃，说明河流沉积物是Vero毒素基因转移和激活的场所。4)铜绿假单胞菌在海洋环境中大量分布。这种细菌应该是这个星球上最丰富的生物，应该在各种环境中发挥重要的基因转移载体作用。5)荧光基因簇划分出若干基因型，这些基因型可能在不同的宿主鱼中转移和进化。6)非特异性基因转移病毒样颗粒存在于多种细菌中，是自然环境中的载体。7)开发了一种新的方法——DDCS，该方法可用于分离具有不同物理状态的细胞。

项目成果

Bacterial population dynamics and community structure in a pharmaceutical manufacturing water supply system by PCR and PCR-denaturing gradient gel electrophoresis.

通过 PCR 和 PCR 变性梯度凝胶电泳研究制药供水系统中的细菌种群动态和群落结构。

• 发表时间: 2004
• 期刊: J.Appl.Microbiol. 97
• 作者: M.Kawai;J.Yamagishi;N.Yamaguchi;K.Tani;M.Nasu
• 通讯作者: M.Nasu

Distribution of oxytetracycline resistance determinant tet34 among bacteria isolated from diseased fish.

从病鱼中分离出的细菌中土霉素抗性决定簇 tet34 的分布。

• 发表时间: 2002
• 期刊: Microb.Environ. 17
• 作者: Nonaka;L.;Isshiki;T.;Suzuki;S.
• 通讯作者: S.

Kim, S-R., Nonaka, L., Oh, M-J, Pitogo, CR, Suzuki, S: "Distribution of oxytetracycline resistance determinant tet(34) among marine bacterial isolates of a Vibrio species."Microbes and Environment. 18. 74-81 (2003)

Kim, S-R.、Nonaka, L.、Oh, M-J、Pitogo, CR、Suzuki, S：“弧菌海洋细菌分离株中土霉素抗性决定簇 tet (34) 的分布。”微生物与环境。

Lisa Nonaka, Satoru Suzuki: "New Mg^<2+>-dependent oxytetracycline resistant determinant Tet34 in Vibrio isolated from marine fish intestinal contents"Antimicrob. Agents Chemother.. 46. 1550-1552 (2002)

Lisa Nonaka，Satoru Suzuki：“从海洋鱼肠道内容物中分离出的弧菌中新的Mg^2依赖的土霉素抗性决定簇Tet34”抗菌剂。

Occurrence of tetracycline resistance gene tet(M) and tet(S) in bacteria from marine aquaculture sites.

海水养殖场细菌中四环素抗性基因 tet(M) 和 tet(S) 的出现。

• 发表时间: 2004
• 期刊: FEMS Microbiology Letters 237
• 作者: Kim SR;Nonaka L;Suzuki S
• 通讯作者: Suzuki S