Study of Haemophilus parainfluenzae antigen and antibody in the etiology of IgA nephropathy

副流感嗜血杆菌抗原抗体在IgA肾病病因学中的研究

• 批准号: 06671135
• 负责人: SUZUKI Satoru
• 金额: $ 1.28万
• 依托单位: Fukui Medical School (1995)Niigata University (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671135/
• 关键词: IgA nephropathy; Haemophilus parainfluenzae; Haemophilus influenzae; Amino acid sequence; Outer membrane; amino acid sequence; outer membrane; IgA腎症; polyclonal activation; IgA class switch

We previously demonstrated a close relationship between the outer membranes of Haemophilus parainfluenzae (HP) antigens (OMHP) and IgA nephropathy. Our objective was to clarify the relationship among IgA-, IgG-, and IgM-class antibody (Ab) against OMHP in the sera of 44 patients with IgA nephropathy and 62 patients with other glomerular diseases (OGD) by enzyme-linked immunosorbent assay (ELISA). Patients with IgA nephropathy showed a significantly higher level of IgA-Ab (P<0.0005) and IgG-Ab (P<0.001) against OMHP,than did patients with OGD.Positive correlations were observed between IgA-and IgG-Ab, between IgA-and IgM-Ab, and between IgG-and IgM-Ab against OMHP in the sera of patients with IgA nephropathy. Immunoblotting showed that IgA-, IgG-, or IgM-Ab against OMHP in the sere of all patients with IgA nephropathy bound to the components of OMHP.Amino acid sequences of three components of OMHP recognized by the sera from patients with IgA nephropathy revealed the homology with those reported for outer membrane protein (OMP) P6 precursor, OMP P5, and P2 porin protein of Haemophilus influenzae.Results suggest that patients with IgA nephropathy have glomerular deposits of OMP P6 precursor, OMP P5, or P2 porin protein of HP,and a specific increase in the production of IgA-Ab against OMHP via polyclonal activation against these, with switching of production from one isotype to another, eg, from IgM to IgA.

我们之前证明了副流感嗜血杆菌（HP）抗原（OMHP）外膜与 IgA 肾病之间的密切关系。我们的目的是通过酶联免疫吸附测定 (ELISA) 阐明 44 例 IgA 肾病患者和 62 例其他肾小球疾病 (OGD) 患者血清中针对 OMHP 的 IgA、IgG 和 IgM 类抗体 (Ab) 之间的关系。 IgA肾病患者抗OMHP的IgA-Ab（P<0.0005）和IgG-Ab（P<0.001）水平显着高于OGD患者。IgA患者血清中针对OMHP的IgA-与IgG-Ab、IgA-与IgM-Ab、IgG-与IgM-Ab之间均呈正相关。 肾病。免疫印迹显示，所有 IgA 肾病患者血清中针对 OMHP 的 IgA-、IgG- 或 IgM-Ab 均与 OMHP 成分结合。IgA 肾病患者血清识别的 OMHP 三种成分的氨基酸序列与报道的外膜蛋白 (OMP) P6 前体、OMP P5、 结果表明，IgA 肾病患者的肾小球中存在 OMP P6 前体、OMP P5 或 HP P2 孔蛋白的沉积，并且通过针对这些物质的多克隆激活，针对 OMHP 的 IgA-Ab 的产生特异性增加，同时将产生从一种同种型转换为另一种同种型，例如从 IgM 转换为 IgA。

项目成果

Suzuki Satoru: "Circulating antibodies against Haemophilus parainfluenzae antigens in IgA nephropathy" XIIIth International Congress of Nephrology. (Abstracts). 318 (1995)

铃木悟：“IgA 肾病中针对副流感嗜血杆菌抗原的循环抗体”第十三届国际肾病学大会。

鈴木亨: "IgA腎症におけるHaemophilus parainfluenzaeb抗原に対する抗体の検討" 日本腎臓学会誌. 36(Suppl.). 41 (1994)

Toru Suzuki：“IgA 肾病中抗副流感嗜血杆菌抗原的抗体检查”，日本肾病学会杂志 36（增刊）。

Suzuki Satoru: "Circulating antibodies against Haemophilus parainfluenzae antigens in IgA nephropathy(IgAN)" XIII th International Congress of Nephrology. Abstracts. 318 (1995)

Suzuki Satoru：“IgA 肾病 (IgAN) 中针对副流感嗜血杆菌抗原的循环抗体”第十三届国际肾脏病学大会。

鈴木 亨: "IgA腎症におけるHaemophilus parainfluenzae抗原に対する抗体の検討" 日本腎臓学会誌. 36(Suppl.). 41 (1994)

Toru Suzuki：“IgA 肾病中抗副流感嗜血杆菌抗原的抗体检查”，日本肾病学会杂志 36（增刊）。

鈴木 亨: "IgA腎症における抗Haemophilus parainfluenzae(HP)抗体価と腎病変との関係およびHP抗原のアミノ酸分析の検討" 日本腎臓学会誌. 37(Suppl.). 29 (1995)

Toru Suzuki：“抗副流感嗜血杆菌（HP）抗体滴度与 IgA 肾病中肾脏病变的关系以及 HP 抗原的氨基酸分析检查”日本肾病学会杂志 37（增刊）。