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Molecular manipulation and application of thermophilic cyanobacteria

Molecular manipulation and application of thermophilic cyanobacteria
嗜热蓝藻的分子操控及应用
批准号:
11554035
负责人:
IKEUCHI Masahiko
金额:
$9.09万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1999
资助国家:
日本
项目状态:
已结题
起止时间:
1999 至 2001

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中文摘要
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英文摘要
A transformation-competent clone of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1 was established. In collaboration with S. Tabata (Kazusa DNA Res. Inst.), we determined the complete nucleotide sequence.A gene for a novel flavin-binding protein was cloned from T. elongatus and expressed in E. coli.Phycocyanin was isolated from T. elongatus and crystalled. X-ray diffraction data at 2.0 A resolution was obtained.Gene disruption mutants were created for psbI, psbT, psbV and psbK in T. elongatus. Photosystem II complexes were isolated from these mutants.A number of site-directed mutants were constructed for the extrinsic 33 kDa protein of T. elongatus and were analyzed functionally.O_2-evolving Photosystem II complex was isolated from wild type of T. vulcanus and crystallized. The 3D
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Katoh, H., S. Itoh, J. R. Shen, and M. Ikeuchi: "Functional analysis of psbV and a Novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus elongatus Strain BP-1"Plant Cell Physiol. 42. 599-607 (2001)
Katoh, H.、S. Itoh、J. R. Shen 和 M. Ikeuchi:“嗜热蓝细菌 Thermosynechococcus elongatus 菌株 BP-1 的 psbV 和新型 c 型细胞色素基因 psbV2 的功能分析”植物细胞生理学。
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通讯作者:
Yoshihara, S., X. Geng, and M. Ikeuchi: "pilG gene cluster and split pilL genes involved in pilus biogenesis, motility and genetic transformation in the cyanobacterium Synechocystis sp. PCC 6803"Plant Cell Physiol. 43. 513-521 (2002)
Yoshihara, S.、X. Geng 和 M. Ikeuchi:“pilG 基因簇和分裂的 pilL 基因参与蓝藻集胞藻 PCC 6803 菌毛生物发生、运动和遗传转化”植物细胞生理学。
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通讯作者:
Ikeuchi, M., Tabata, S.: "Synechocystis sp. PCC 6803 -a useful tool in the study of the genetics of cyanobacteria"Photosynthesis Research. 70. 73-83 (2001)
Ikeuchi, M., Tabata, S.:“集胞藻属 sp. PCC 6803 - 蓝藻遗传学研究的有用工具”光合作用研究。
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Umeda et al.: "Cloning of cellulose synthase genes from Acetobacter・・・"DNA Research. 6. 109-115 (1999)
Umeda 等人:“从醋杆菌中克隆纤维素合酶基因……”DNA Research 6. 109-115 (1999)。
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29
    Acidosis induced pain and mechanisms of joint destruction
    • 批准号:
      26460698
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.33万
    • 财政年份:
      2014
    • 负责人:
      IKEUCHI Masahiko
    • 依托单位:
    Integrated analysis of cyanobacteriochromes in diversity and molecular regulation of cyanobacterial photoresponses
    • 批准号:
      23370014
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.9万
    • 财政年份:
      2011
    • 负责人:
      IKEUCHI Masahiko
    • 依托单位:
    Role of synovial fluid acidity and acid sensing ion channel in osteoarthritic pain
    • 批准号:
      21791401
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $1.16万
    • 财政年份:
      2009
    • 负责人:
      IKEUCHI Masahiko
    • 依托单位:
    Study of photoperception mechanism of a novel photoreceptor, cyanobacteriochrome family
    • 批准号:
      20370018
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $10.32万
    • 财政年份:
      2008
    • 负责人:
      IKEUCHI Masahiko
    • 依托单位:
    海外基金