Molecular manipulation and application of thermophilic cyanobacteria

嗜热蓝藻的分子操控及应用

• 批准号: 11554035
• 负责人: IKEUCHI Masahiko
• 金额: $ 9.09万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11554035/
• 关键词: cyanobacteria; blue-green algae; photosynthetic production; thermophile; gene manipulation; gene expression; photosystem II; crystallization; シャトルベクター

A transformation-competent clone of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1 was established. In collaboration with S. Tabata (Kazusa DNA Res. Inst.), we determined the complete nucleotide sequence.A gene for a novel flavin-binding protein was cloned from T. elongatus and expressed in E. coli.Phycocyanin was isolated from T. elongatus and crystalled. X-ray diffraction data at 2.0 A resolution was obtained.Gene disruption mutants were created for psbI, psbT, psbV and psbK in T. elongatus. Photosystem II complexes were isolated from these mutants.A number of site-directed mutants were constructed for the extrinsic 33 kDa protein of T. elongatus and were analyzed functionally.O_2-evolving Photosystem II complex was isolated from wild type of T. vulcanus and crystallized. The 3D

建立了嗜热蓝藻细长嗜热菌BP-1的转化能力克隆。我们与Tabata(Kazusa DNA Res.Inst.)合作，测定了该基因的全序列，克隆了一个新的黄素结合蛋白基因，并在大肠杆菌中进行了表达，分离得到了藻蓝蛋白，并进行了结晶。获得了2.0A分辨率的X射线衍射谱数据，并对长穗拟青霉中的pSBI、psbT、psbV和psbK基因进行了突变。从这些突变体中分离出光系统II复合体，构建了一系列针对长穗金龟子外源33 kDa蛋白的定点突变体，并对其进行了功能分析；从野生株中分离出放氧光系统II复合体并结晶。3D

项目成果

Katoh, H., S. Itoh, J. R. Shen, and M. Ikeuchi: "Functional analysis of psbV and a Novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus elongatus Strain BP-1"Plant Cell Physiol. 42. 599-607 (2001)

Katoh, H.、S. Itoh、J. R. Shen 和 M. Ikeuchi：“嗜热蓝细菌 Thermosynechococcus elongatus 菌株 BP-1 的 psbV 和新型 c 型细胞色素基因 psbV2 的功能分析”植物细胞生理学。

Yoshihara, S., X. Geng, and M. Ikeuchi: "pilG gene cluster and split pilL genes involved in pilus biogenesis, motility and genetic transformation in the cyanobacterium Synechocystis sp. PCC 6803"Plant Cell Physiol. 43. 513-521 (2002)

Yoshihara, S.、X. Geng 和 M. Ikeuchi：“pilG 基因簇和分裂的 pilL 基因参与蓝藻集胞藻 PCC 6803 菌毛生物发生、运动和遗传转化”植物细胞生理学。

Ikeuchi, M., Tabata, S.: "Synechocystis sp. PCC 6803 -a useful tool in the study of the genetics of cyanobacteria"Photosynthesis Research. 70. 73-83 (2001)

Ikeuchi, M., Tabata, S.：“集胞藻属 sp. PCC 6803 - 蓝藻遗传学研究的有用工具”光合作用研究。

大森正之, 池内昌彦: "タンパク質・核酸・酵素(分担)"シアノバクテリアの光合成遺伝子・タンパク質システムの解明. 2492 (2001)

Masayuki Omori、Masahiko Ikeuchi：“蛋白质、核酸和酶（共享）”蓝藻光合基因和蛋白质系统的阐明 2492 (2001)。

Umeda et al.: "Cloning of cellulose synthase genes from Acetobacter・・・"DNA Research. 6. 109-115 (1999)

Umeda 等人：“从醋杆菌中克隆纤维素合酶基因……”DNA Research 6. 109-115 (1999)。