Molecular biology of light-responsive genes in cyanobacteria

蓝藻光响应基因的分子生物学

• 批准号: 10640626
• 负责人: IKEUCHI Masahiko
• 金额: $ 2.11万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640626/
• 关键词: cyanobacteria; blue-greem algae; photosynthesis; high light acclimation; respiration; reactive oxygen species

Phototrophic organisms respond to various light environments for the purpose of optimization of photosynthesis directly or indirectly. However, the molecular mechanism of light acclimation has not yet been elucidated in detail. We took advantage of transformable cyanobacterium Synechocystis sp.PCC 6803 for molecular analysis of light-responsive mechanism. Genes we have identified in this study are slr2031 (putative Ser/Thr protein phosphatase), spkA (Ser/Thr protein kinase), spkB (Ser/Thr protein kinase), pisJl operon (phytochrome-like photoreceptor and signal transduction pathway genes). We also analyzed high light-responsive genes by using DNA microarray. Through our works, outline of the whole image was given for molecular mechanism with which cyanobacteria respond to light environments.

光营养生物对各种光环境做出反应，目的是直接或间接优化光合作用。然而，光适应的分子机制尚未详细阐明。我们利用了可转化的蓝细菌合成藻SP.PCC 6803进行光反应机制的分子分析。我们在这项研究中鉴定的基因是SLR2031（推定的Ser/Thr蛋白磷酸酶），SPKA（Ser/Thr蛋白激酶），SPKB（Ser/Thr蛋白激酶），PISJL操纵子（植物色素样光疗法和信号转移传递途径）。我们还使用DNA微阵列分析了高光反应基因。通过我们的作品，给出了整个图像的轮廓，用于分子机制，蓝细菌对光环境有反应。

项目成果

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