Studies of phytochromes and signal transductbn pathways in cyanobacterial phototaxis

蓝藻趋光性光敏色素和信号转导途径的研究

• 批准号: 13440235
• 负责人: IKEUCHI Masahiko
• 金额: $ 9.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440235/
• 关键词: cyanobacteria; blue-green algae; phototaxis; flavin sensor; photoconversion; phytochrome; photoreceptor; phycocyanin; 運動性; 形質転換能; 分割遺伝子

A gene for a novel flavin-binding protein that is involved in positive phototaxis was cloned from T. elongatus and expressed in E. coli, and expressed. The protein was crystallized and resolved at 2.0 A.Novel phytochrome-like gene pixA was identified as a putative photoreceptor for positive phototaxis. Regulator proteins encoded by nixB and nixC are hypothesized by combinatorial mutagenesis.Regulator genes essential for assembly of motility pili were identified as follows : pilG, pilH, pill, pilJ, pilL-N and pilL-C. The latter two genes were separately expressed on distinct loci on the chromosome.Positive phototaxis photoreceptor PixJ1 protein was isolated from Synechocystis sp. PCC 6803. The protein thus isolated showed novel absorption spectra and difference spectrum in blue and green light regions. The chromophore of PixJ1 was concluded to be a derivative of phycocyanobilin.Four novel flavin-binding photoreceptor proteins were expressed heterologously and their photocycles were partially characterized.The PAS domain proteins of a filamentous cyanobacterium Anabaena sp. PCC 7120 were systematically studied by bioinformatics and biochemical analyses.Genes for the pili subunits were systematically disrupted and identified as pil9,pi110, and pil11.

本研究克隆了一种新的与正趋光性有关的黄素结合蛋白基因，并在大肠杆菌中表达。该蛋白在2.0A结晶和分解。新的光敏素样基因PixA被鉴定为正趋光性的光感受器。用组合突变的方法推测了nixB和nixC编码的调控蛋白，确定了与运动菌毛组装有关的调控基因：PilG、PilH、Pill、PilJ、Pill-N和Pill-C。后两个基因分别在染色体上的不同位点表达。从集胞藻中分离到阳性趋光性感光蛋白PixJ1。PCC 6803。分离得到的蛋白质在蓝绿光区表现出新的吸收光谱和差光谱。PixJ1的发色团是藻蓝胆蛋白的衍生物。异源表达了4种新的黄素结合光受体蛋白，并对它们的光周期进行了部分鉴定。通过生物信息学和生化分析对PCC7120进行了系统研究，对菌毛亚基基因进行了系统打乱，鉴定为Pil9、Pi110和Pil11。

项目成果

大森正之, 池内昌彦: "タンパク質・核酸・酵素(分担)"シアノバクテリアの光合成遺伝子・タンパク質システムの解明. 2492 (2001)

Masayuki Omori、Masahiko Ikeuchi：“蛋白质、核酸和酶（共享）”蓝藻光合基因和蛋白质系统的阐明 2492 (2001)。

Molecular evolution of PAS domain-containing proteins of filamentous cyanobacteria through the domain shuffling and domain duplication

通过结构域改组和结构域复制实现丝状蓝细菌 PAS 结构域蛋白的分子进化

• 发表时间: 2004
• 期刊: DNA Research 11
• 作者: Iwai et al.;Kobayashi et al.;Sato et al.;Narikawa et al.
• 通讯作者: Narikawa et al.

Kobayashi et al.: "Response to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis sp. PCC 6803"Plant Cell Physiology. 45(In press). (2004)

Kobayashi 等人：“对氧化应激的反应涉及单细胞蓝藻集胞藻属 PCC 6803 中的一种新型过氧化还原蛋白基因”植物细胞生理学。

Use of segment-based microarray in the analysis of global gene expression in response to various environmental stresses in the cyanobacterium Anabaena sp PCC 7120

• DOI: 10.2323/jgam.50.1
• 发表时间: 2004-02-01
• 期刊: JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
• 影响因子: 1.2
• 作者: Sato, N;Ohmori, M;Kuhara, S
• 通讯作者: Kuhara, S

Biochemical and functional characterization of a eukaryotic-type protein kinase, SpkB, in the cyanobacterium, Synechocystis sp.

蓝藻集胞藻中真核型蛋白激酶 SpkB 的生化和功能特征。

• 发表时间: 2003
• 期刊: PCC 6803. Curr Microbiol 46
• 作者: Kamei A;Yoshihara S;Yuasa T;Geng X;Ikeuchi M
• 通讯作者: Ikeuchi M