Studies of phytochromes and signal transductbn pathways in cyanobacterial phototaxis

蓝藻趋光性光敏色素和信号转导途径的研究

• 批准号: 13440235
• 负责人: IKEUCHI Masahiko
• 金额: $ 9.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440235/
• 关键词: cyanobacteria; blue-green algae; phototaxis; flavin sensor; photoconversion; phytochrome; photoreceptor; phycocyanin; 運動性; 形質転換能; 分割遺伝子

A gene for a novel flavin-binding protein that is involved in positive phototaxis was cloned from T. elongatus and expressed in E. coli, and expressed. The protein was crystallized and resolved at 2.0 A.Novel phytochrome-like gene pixA was identified as a putative photoreceptor for positive phototaxis. Regulator proteins encoded by nixB and nixC are hypothesized by combinatorial mutagenesis.Regulator genes essential for assembly of motility pili were identified as follows : pilG, pilH, pill, pilJ, pilL-N and pilL-C. The latter two genes were separately expressed on distinct loci on the chromosome.Positive phototaxis photoreceptor PixJ1 protein was isolated from Synechocystis sp. PCC 6803. The protein thus isolated showed novel absorption spectra and difference spectrum in blue and green light regions. The chromophore of PixJ1 was concluded to be a derivative of phycocyanobilin.Four novel flavin-binding photoreceptor proteins were expressed heterologously and their photocycles were partially characterized.The PAS domain proteins of a filamentous cyanobacterium Anabaena sp. PCC 7120 were systematically studied by bioinformatics and biochemical analyses.Genes for the pili subunits were systematically disrupted and identified as pil9,pi110, and pil11.

与阳性光的新型黄素结合蛋白的一种基因是从T. elongatus克隆并在大肠杆菌中表达并表达的。将蛋白质结晶并在2.0 A. Novel植物色素样基因PixA鉴定为阳性光阳性的假定光感受器。由NIXB和NIXC编码的调节蛋白通过组合诱变来假设。调节基因在运动pili的组装中必不可少的基因如下：PILG，PILH，PILH，PILH，PILL，PILJ，PILJ，PILJ，PILL-N和PIRL-C。后两个基因在染色体上的不同基因座上分别表达。阳性光感应光感受器PIXJ1蛋白是从Synechocystis sp。分离的。 PCC 6803。这样分离的蛋白质显示出新的吸收光谱和蓝色和绿光区域的差异光谱。 PIXJ1的发色团得出结论是植物苯胺蛋白的导数。 PCC 7120通过生物信息学和生化分析系统地研究了PILI亚基的基因，被系统破坏并确定为PIL9，PI110和PIL11。

项目成果

Molecular evolution of PAS domain-containing proteins of filamentous cyanobacteria through the domain shuffling and domain duplication

通过结构域改组和结构域复制实现丝状蓝细菌 PAS 结构域蛋白的分子进化

• 发表时间: 2004
• 期刊: DNA Research 11
• 作者: Iwai et al.;Kobayashi et al.;Sato et al.;Narikawa et al.
• 通讯作者: Narikawa et al.

Kobayashi et al.: "Response to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis sp. PCC 6803"Plant Cell Physiology. 45(In press). (2004)

Kobayashi 等人：“对氧化应激的反应涉及单细胞蓝藻集胞藻属 PCC 6803 中的一种新型过氧化还原蛋白基因”植物细胞生理学。

Kamei, A. et al.: "Biochemical and functional characterization of a eukaryotic-type protein kinase, SpkB, in the cyanobacterium, Synechocystis sp. PCC 6803"Current Microbiology. (in press). (2003)

Kamei, A. 等人：“蓝藻集胞藻 PCC 6803 中真核型蛋白激酶 SpkB 的生化和功能特征”当前微生物学。

Biochemical and functional characterization of a eukaryotic-type protein kinase, SpkB, in the cyanobacterium, Synechocystis sp.

蓝藻集胞藻中真核型蛋白激酶 SpkB 的生化和功能特征。

• 发表时间: 2003
• 期刊: PCC 6803. Curr Microbiol 46
• 作者: Kamei A;Yoshihara S;Yuasa T;Geng X;Ikeuchi M
• 通讯作者: Ikeuchi M

Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1

• DOI: 10.1093/pcp/pch015
• 发表时间: 2004-02-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Iwai, M;Katoh, H;Ikeuchi, M
• 通讯作者: Ikeuchi, M