Integrated analysis of cyanobacteriochromes in diversity and molecular regulation of cyanobacterial photoresponses

蓝藻色素多样性和蓝藻光反应分子调控的综合分析

• 批准号: 23370014
• 负责人: IKEUCHI Masahiko
• 金额: $ 11.9万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370014/
• 关键词: 受容体; 結晶構造; シグナル伝達; c-di-GMP; フィトクロム; シアノバクテリオクロム; 光受容体; シアノバクテリア; 走光性; 補色順化; 細胞凝集

We found that cyanobacteria possess diverse photoreceptors and cyanobacteriochromes are unique in perception of wide range of light color. We focused on biochemical, structural and physiological aspects of cyanobacteriochromes in order to integrate the light signaling system at molecular level. We determined the crystal structures of two cyanobacteriochromes and revealed the atomic basis for the color tuning and intramolecular signaling. We determined enzymatic regulation of the cyanobacteriochromes to produce or degrade a second messenger molecule, cyclic diguanylic acid, which induces cellulose production and cellular aggregation in thermophilic cyanobacteria. We also elucidated the molecular contribution of the cyanobacteriochromes to the cellular aggregation by combinatorial mutagenesis studies. Thus, a set of cyanobacteriochromes integrates various light signals into the cellulose production and cellular aggregation by direct regulation of cyclic diguanylic acid.

我们发现蓝细菌具有多样的光感受器，蓝细菌色素在感知大范围的光颜色方面是独特的。本文从蓝藻色素的生物化学、结构和生理学等方面对蓝藻色素进行了研究，以期在分子水平上整合光信号系统。我们确定了两个蓝藻色素的晶体结构，并揭示了颜色调谐和分子内信号的原子基础。我们确定的蓝藻色素的酶促调节产生或降解的第二信使分子，环二鸟苷酸，诱导纤维素生产和细胞聚集在嗜热蓝藻。我们还阐明了蓝藻色素的分子贡献的细胞聚集的组合诱变研究。因此，一组蓝藻色素通过直接调节环状二鸟苷酸将各种光信号整合到纤维素生产和细胞聚集中。

项目成果

CugP Is a Novel Ubiquitous Non-GalU-Type Bacterial UDP-Glucose Pyrophosphorylase Found in Cyanobacteria

• DOI: 10.1128/jb.01591-14
• 发表时间: 2014-04
• 期刊: Journal of Bacteriology
• 影响因子: 3.2
• 作者: Kaisei Maeda;R. Narikawa;M. Ikeuchi

Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation.

• DOI: 10.1038/ncomms4978
• 发表时间: 2014-05-28
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Hori K;Maruyama F;Fujisawa T;Togashi T;Yamamoto N;Seo M;Sato S;Yamada T;Mori H;Tajima N;Moriyama T;Ikeuchi M;Watanabe M;Wada H;Kobayashi K;Saito M;Masuda T;Sasaki-Sekimoto Y;Mashiguchi K;Awai K;Shimojima M;Masuda S;Iwai M;Nobusawa T;Narise T;Kondo S;Saito H;Sato R;Murakawa M;Ihara Y;Oshima-Yamada Y;Ohtaka K;Satoh M;Sonobe K;Ishii M;Ohtani R;Kanamori-Sato M;Honoki R;Miyazaki D;Mochizuki H;Umetsu J;Higashi K;Shibata D;Kamiya Y;Sato N;Nakamura Y;Tabata S;Ida S;Kurokawa K;Ohta H
• 通讯作者: Ohta H

The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp. strain HK-01 in NaCl tolerance

• DOI: 10.1007/s10811-011-9672-5
• 发表时间: 2011-04
• 期刊: Journal of Applied Phycology
• 影响因子: 3.3
• 作者: Hidehisa Yoshimura;Toshihisa Kotake;Tsutomu Aohara;Y. Tsumuraya;M. Ikeuchi;M. Ohmori

A deletion mutation in the spacing within the psaA core promoter enhances transcription in a cyanobacterium Synechocystis sp. PCC 6803.

• DOI: 10.1093/pcp/pcr159
• 发表时间: 2012
• 期刊: Plant & cell physiology
• 影响因子: 4.9
• 作者: T. Midorikawa;R. Narikawa;M. Ikeuchi

Genetic engineering of the group 2 sigma factor SigE widely activates the expressions of sugar catabolic genes in Synechocystis sp. PCC 6803

2组σ因子SigE的基因工程广泛激活集胞藻中糖分解代谢基因的表达。

• 发表时间: 2011
• 期刊: J Biol Chem
• 影响因子: 4.8
• 作者: Osanai;T.;Oikawa;A.;Azuma;M.;Tanaka;K.;Saito;K.;Hirai;M. Y.;and Ikeuchi;M
• 通讯作者: M